Method for preparing high-sensitivity immunity quantitative latex testing reagent

Abstract

The invention relates to a method for preparing a high-sensitivity immunity quantitative latex testing reagent. The reagent comprises the following components: microsphere particles of which the concentration is between 0.2 and 0.4 percent (w / w), 0.12 M of glycine buffer solution of which the pH is 7.2, 30 percent of ethylene glycol (v / v), 2mM EDTA.2Na disodium EDTA, and 0.1 percent of NaN3. The preparation method comprises the following steps: preparing vector immunity quantitative latex micro-particles; preparing an antibody and an antigen; adding EDC at normal temperature; activating carboxyl under a condition that the pH is less than 6.5; and after certain period of time, raising the pH value and slowing down the reaction. Compared with the prior art, the method for preparing the high-sensitivity immunity quantitative latex testing reagent improves the uniformity of the diameters of the vector micro-particles and the uniformity of the antigen or the antibody distributed on the surfaces of the particles, and simultaneously improves the degree of freedom of the coated antigen or the antibody under a homogeneous phase condition, reduces the spatial combination steric hindrance, improves the secondary immunological reaction rate and improves the reaction sensitivity, and the detection sensitivity is improved by 100 times compared with that reported by the literatures in the past.

Description

Technical field: [0001] The invention relates to a high-sensitivity immune latex quantitative turbidimetric assay reagent, in particular to a preparation method of the reagent. Background technique: [0002] Since the development of particles with a diameter of less than 200 nanometers in the 1980s can be used as a homogeneous reagent for immune carriers, many immunoassay projects have been developed. The advantages are as follows: First, because the particle diameter is less than 2 / 5 of the wavelength of light, both It can be used as a carrier of immune substances, and it can be regarded as a homogeneous reagent, which has obvious advantages in automatic quantitative operation; the second is the solid phase of immune substances, which reduces the free energy of the antigen or antibody of the loaded substance and significantly prolongs the immunity of the substance to the antigen or antibody. Antibody activity, due to the above advantages, many immunoassay projects have succ...

AI Technical Summary

Problems solved by technology

However, its disadvantage is that it is not as sensitive as enzyme labeling and fluorescent labeling methods. It cannot detect protein molecules with very low content, and cannot form an effective immune binding network for small molecules of hormones and drugs, so it cannot be used. The method detects

Similarly, there are certain difficulties in the use of monoclonal antibodies, which require multiple monoclonal antibodies to be used in combination

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