Method for preparing high-sensitivity immunity quantitative latex testing reagent

An immunoquantitative and sensitive technology, applied in biological tests, measuring devices, material testing products, etc., can solve the problems of insufficient sensitivity, inability to form immune binding networks, and inability of protein molecules to improve sensitivity and increase secondary immune responses. speed, and the effect of improving the sensitivity of the reaction

Inactive Publication Date: 2010-01-13
JIANGSU FLON BIOTECH
View PDF0 Cites 24 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its disadvantage is that it is not as sensitive as enzyme labeling and fluorescent labeling methods. It cannot detect protein molecules with very low content, and cannot form an effective immune binding network for sm

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing high-sensitivity immunity quantitative latex testing reagent

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0015] (1) Preparation of Carrier Immunoquantitative Latex Microparticles Pure water is heated to boiling to remove most of the oxygen in the water. After cooling, take 750ml and add 1.0g sodium dodecylsulfonate SDS to keep the temperature at 65°C for 10 minutes. After the SDS is in a suspended solution state, add 30ml of styrene monomer with a content greater than 98%, then raise the temperature to 75°C, and add 5.2ml of acrylic acid (pH adjusted to 5.5-6.5) after equilibrating for 15 minutes, and then add potassium persulfate after equilibrating. Start timing and gradually raise the temperature to 84°C. The reaction time is 30 minutes. Add neutralized acrylic acid in two times according to 4 times the amount of carboxyl groups on the surface of the microparticle size until the surface of the microspheres is fully carboxylated. Aging at 84°C for two hours , natural cooling. Calculate the concentration of the reaction based on the effective amount added to the reaction and the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for preparing a high-sensitivity immunity quantitative latex testing reagent. The reagent comprises the following components: microsphere particles of which the concentration is between 0.2 and 0.4 percent (w/w), 0.12 M of glycine buffer solution of which the pH is 7.2, 30 percent of ethylene glycol (v/v), 2mM EDTA.2Na disodium EDTA, and 0.1 percent of NaN3. The preparation method comprises the following steps: preparing vector immunity quantitative latex micro-particles; preparing an antibody and an antigen; adding EDC at normal temperature; activating carboxyl under a condition that the pH is less than 6.5; and after certain period of time, raising the pH value and slowing down the reaction. Compared with the prior art, the method for preparing the high-sensitivity immunity quantitative latex testing reagent improves the uniformity of the diameters of the vector micro-particles and the uniformity of the antigen or the antibody distributed on the surfaces of the particles, and simultaneously improves the degree of freedom of the coated antigen or the antibody under a homogeneous phase condition, reduces the spatial combination steric hindrance, improves the secondary immunological reaction rate and improves the reaction sensitivity, and the detection sensitivity is improved by 100 times compared with that reported by the literatures in the past.

Description

Technical field: [0001] The invention relates to a high-sensitivity immune latex quantitative turbidimetric assay reagent, in particular to a preparation method of the reagent. Background technique: [0002] Since the development of particles with a diameter of less than 200 nanometers in the 1980s can be used as a homogeneous reagent for immune carriers, many immunoassay projects have been developed. The advantages are as follows: First, because the particle diameter is less than 2 / 5 of the wavelength of light, both It can be used as a carrier of immune substances, and it can be regarded as a homogeneous reagent, which has obvious advantages in automatic quantitative operation; the second is the solid phase of immune substances, which reduces the free energy of the antigen or antibody of the loaded substance and significantly prolongs the immunity of the substance to the antigen or antibody. Antibody activity, due to the above advantages, many immunoassay projects have succ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/68G01N33/546G01N21/17
Inventor 李惠福
Owner JIANGSU FLON BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap