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Method for extracting effective components from natural products by adopting triple liquid phases

A technology of natural products and active ingredients, applied in the field of bioengineering, can solve problems such as high cost, large amount of solvent used, and low extraction efficiency, and achieve the effects of easy recovery, cheap recovery, and improved extraction and separation efficiency

Inactive Publication Date: 2010-02-03
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a hydrophobic organic matter / hydrophilic low molecular organic matter / inorganic salt / water three A method for simultaneously extracting and separating two or more active ingredients from a natural product or its crude extract by a liquid phase system

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Embodiment 1: the extraction and separation of capsaicin and capsanthin

[0011] Step 1: Pulverize the dried chili and pass through a 60-mesh sieve to obtain dried chili powder.

[0012] Step 2: Get 3.6g of anhydrous dipotassium hydrogen phosphate and 10.8g of water to make dipotassium hydrogen phosphate solution.

[0013] Step 3 Add the above-mentioned inorganic salt solution, 3.6g of absolute ethanol and 2.0g of n-hexane into 1.0g of dried chili powder, stir evenly to form a three-phase system, and stand at room temperature for 2.0h of phase separation. 91% of capsaicin is distributed in the upper phase (n-hexane phase), 93% of capsaicin is distributed in the middle phase (ethanol phase), and the capsicum residue is between the middle phase and the lower phase. Other components, especially polar The impurities remain in the lower phase (salt phase).

Embodiment 2

[0014] Embodiment 2: the extraction and separation of capsaicin and capsanthin

[0015] Step 1: Pulverize the dried chili and pass through a 60-mesh sieve to obtain dried chili powder.

[0016] Step 2 Take 1.0 g of chili powder, extract with 33.0 g of dioxane under reflux at 50° C. for 1.0 h, three times in total, filter, and combine the filtrates to obtain the dioxane extract of dried chili.

[0017] Step 3 prepares a dipotassium hydrogen phosphate solution with a mass fraction of 33.3%.

[0018] Step 4 Mix 6.4 g of the above inorganic salt solution, 9.6 g of dioxane extract and 4.0 g of n-hexane, stir evenly to form a three-phase system, and stand at room temperature for 2.0 h of phase separation. 100% capsaicin is distributed in the upper phase (n-hexane phase), and 100% capsaicin is distributed in the middle phase (dioxane phase). There is no residue in this system, and other components, especially highly polar impurities, are Stay in the lower phase (salt phase). Capsa...

Embodiment 3

[0019] Embodiment 3: salvianolic acid B and tanshinone II A separation

[0020] Step 1: crush Salvia miltiorrhiza through a 60-mesh sieve to obtain Salvia miltiorrhiza powder, use 82% ethanol solution with a pH of 2.0 to reflux extract at 65°C for 1.5 h, the ratio of solid to liquid is 1:10, filter the residue, and then use water with a pH of 2.0 Reflux extraction at 95°C for 0.5h, the ratio of solid to liquid is 1:20, filter, and combine the two extracts to obtain the crude extract of Salvia miltiorrhiza.

[0021] In step 2, the mass fraction of ethanol in the crude extract of Salvia miltiorrhiza was measured to be 28%. Take 6.24 g of the crude extract, add 1.76 g of solid ammonium sulfate, and mix to dissolve it.

[0022] Step 3: Add 2.0 g of n-hexane to the above mixture, stir evenly to form a three-phase system, and stand at room temperature for phase separation for 1.5 h. 92.7% Tanshinone II A Be distributed in upper phase (n-hexane phase), the salvianolic acid B of 93...

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Abstract

A method for extracting effective components from natural products by adopting triple liquid phases belongs to the technical field of bioengineering. The method is characterized by directly adding inorganic salt solutions, hydrophilic low-molecular organics and hydrophobic organics into ground natural products in proportion, or adding solid inorganic salts and hydrophobic organics into the extractof the hydrophilic low-molecular organics of the natural products in proportion, evenly stirring the mixture, carrying out standing or centrifuging on the mixture to form triple liquid phases; the effective components are enriched on the top phase, middle phase and bottom phase respectively based on different polarity and distributing partial protein, and polysaccharides and crude drug residue among phase interfaces to achieve the purpose of simultaneously extracting the effective components with different polarity from the natural products. The invention has the effects and benefits of developing an easily industrialized method for extracting and separating the effective components from the natural products, increasing the extraction efficiency, effectively combining the technologies ofextraction, separation and purification and being beneficial to reducing the production cost of the effective components from the natural products.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a method for extracting active ingredients from natural products, in particular to a method for three-liquid phase extraction of active ingredients in natural products. Background technique [0002] Active ingredients of natural products refer to substances that are biologically active and can be used as medicinal or nutritional health value, generally based on plants, and active ingredients include polysaccharides, proteins, alkaloids, flavonoids, anthraquinones and tea quinones, Terpenes and steroids, saponins, coumarins and lipids, lignans, cardiac glycosides, phenolic acids, glycosides, volatile oils, pigments, etc. [0003] The extraction method of active ingredients of natural products is mainly solvent extraction, and its separation principle is mainly based on the difference in the distribution characteristics of substances in two immiscible liquid phases. For activ...

Claims

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Application Information

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IPC IPC(8): B01D11/04
Inventor 修志龙张华刘琳梁志霞董悦生
Owner DALIAN UNIV OF TECH
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