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Method for producing biologic surfactant by fermenting desized wastewater

A technology for biological surface and desizing wastewater, applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve problems such as toxicity, growth requirements, and non-compliance with the development trend of printing and dyeing auxiliaries, and achieve low cost, Excellent effect

Inactive Publication Date: 2013-04-03
DONGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Almost every process in the dyeing and finishing process requires the use of surfactants, but the surfactants used at this stage are chemically synthesized and have certain toxicity, which is increasingly inconsistent with the current development of printing and dyeing auxiliaries in the direction of green environmental protection trends, and the performance of surfactants cannot meet the growing requirements of people

Method used

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  • Method for producing biologic surfactant by fermenting desized wastewater
  • Method for producing biologic surfactant by fermenting desized wastewater
  • Method for producing biologic surfactant by fermenting desized wastewater

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Production of lipopeptide biosurfactants by fermentation of desizing wastewater:

[0031] (1) Strain: In this example, the microbial strain used for wastewater fermentation is Bacillus subtilis HSO121. It is a strain producing lipopeptide biosurfactants.

[0032] (2) Culture medium: optimize the medium of Bacillus subtilis HSO121 by simulating the composition of wastewater, and obtain the best medium formula (1L): starch 20g KH 2 PO 4 2.7g Na 2 HPO 4 12H 2 O 17g NH 4 Cl 2gMgSO 4 0.2g NaCl 10g CaCl 2 0.00077g MnSO 4 h 2 O 0.0017g Na 2 EDTA 0.0015g.

[0033] (3) Wastewater pretreatment: take the alkali desizing wastewater of a printing and dyeing factory, the main component of the size is starch. Use HCl to adjust the pH value of the wastewater to neutral, and measure the content of sugar and starch in the wastewater, then heat and concentrate to the concentration required by the medium formula, and filter to remove solid impurities. After pretreatment, o...

Embodiment 2

[0037] Lipopeptide biosurfactant as soaping agent for fabrics dyed with reactive yellow 3RS:

[0038] In this example, the lipopeptide biosurfactant produced in Example 1 was introduced as a soaping aid.

[0039] (1) Preparation of fully hydrolyzed dye: take reactive dye reactive yellow 3RS, prepare 2g / L reactive dye solution with tap water, then adjust pH value to 10-11 with NaOH, heat to 100°C, under boiling conditions Insulate and hydrolyze for 120 minutes, cool down to 60°C, and then adjust the pH value to 7-8 with hydrochloric acid for later use.

[0040] (2) Dyeing process:

[0041] Dyeing prescription: fully hydrolyzed reactive yellow 3RS dye 5% (to fabric weight), NaCl 40g / l, NaCl 2 CO 3 10g / l, liquor ratio 1:50.

[0042] Dye at 60°C, add sodium chloride after 15 minutes, continue dyeing at 60°C for 15 minutes, add soda ash after heating up to 90°C, fix the color at 90°C for 30 minutes, cool down after dyeing, take out the sample, wash and dry Dry.

[0043] (3) So...

Embodiment 3

[0059] Soaping of fabrics dyed with reactive blue BRF using lipopeptide biosurfactants:

[0060] In this example, the lipopeptide biosurfactant produced in Example 1 was introduced as a soaping aid.

[0061] (1) Preparation of fully hydrolyzed dye: take reactive dye reactive blue BRF, prepare 2g / L reactive dye solution with tap water, then use NaOH to adjust the pH value to 10-11, heat to 100°C, under boiling conditions Insulate and hydrolyze for 120 minutes, cool down to 60°C, and then adjust the pH value to 7-8 with hydrochloric acid for later use.

[0062] (2) Dyeing process:

[0063] Dyeing prescription: fully hydrolyzed reactive blue BRF dye 5% (to fabric weight), NaCl 40g / l, NaCl 2 CO 3 10g / l, liquor ratio 1:50.

[0064] Dye at 60°C, add sodium chloride after 15 minutes, continue dyeing at 60°C for 15 minutes, add soda ash after heating up to 90°C, fix the color at 90°C for 30 minutes, cool down after dyeing, take out the sample, wash and dry Dry.

[0065] (3) Soa...

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Abstract

The invention discloses a method for producing a biologic surfactant by fermenting desized wastewater. The method comprises the following steps: taking a large amount of starch-contained desized wastewater generated in printing and dyeing mills as a carbon source for the growth of microorganisms, fermenting and producing the biologic surfactant; taking the desized wastewater with the mass percentof starch of 30-100% of pulp in the printing and dyeing mills after filtering, concentrating and neutralizing as the carbon source of a culture medium, then adding other nutrient substances required by the growth of the microorganisms, inoculating with seed liquid of microbial strains which can generate surface active substances by metabolism after sterilizing, and obtaining fermentation liquid containing the biologic surfactant after fermenting by an optimized fermentation process; and after processing by simple methods, such as centrifugal sterilization, acid precipitation, or decoloration and the like, applying to dyeing and finishing processes, such as processes of boiling off, soaping, soft finishing, antistatic processing and the like. The invention has simple preparation method andlow cost, reduces the environment pollution and is suitable for industrialized production.

Description

technical field [0001] The invention belongs to the field of fermentative production of lipopeptide biosurfactants, in particular to a method for fermenting and producing biosurfactants using desizing wastewater as a medium component. Background technique [0002] Wastewater treatment in dyeing and finishing plants has always been a key and difficult point in environmental protection. At present, wastewater is basically treated through some physical, chemical or biological methods to meet discharge standards. Among them, there are also relevant literatures on the existing desizing wastewater of printing and dyeing factories being recycled, but there is no literature report to use it as a culture medium for microorganisms to produce biosurfactants. [0003] Surfactants occupy a special position in many industrial fields, especially in the textile and printing and dyeing industries. Surfactants may need to be added in various processes. However, surfactants have been synthesi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02C12P1/04B01F17/00D06M16/00C12R1/645C12R1/72C12R1/06C12R1/19C12R1/125C12R1/07C12R1/38C09K23/00
Inventor 陆大年李名健
Owner DONGHUA UNIV