A kind of leaf disc direct regeneration method of Jatropha curcas

A technology of jatropha and leaves, applied in the field of direct regeneration of leaf discs of Jatropha curcas, can solve the problems of narrow distribution and low yield, restricting large-scale cultivation and industrialization, and low yield of Jatropha curcas, to alleviate the energy and energy crisis, The effect of easy promotion and fast regeneration
CN101658139BActive Publication Date: 2011-11-30CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
Publication Date
2011-11-30

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Abstract

The invention discloses a method for directly regenerating leaf discs of Jatropha curcas. It selects annual Jatropha curcas leaves in the greenhouse as explants and inoculates them in the medium to directly induce adventitious buds; the medium is MS basic medium with 30g·L-1 sucrose, 4-8g·L-1 agar, 0.2 -2.0mg·L-1TDZ, 0.1-1.5mg·L-1IBA, 0.05-3mg·L-1BA, 0.5-8mg·L-1 Sodium Nitroprusside; culture method: first cultivate in dark for 10-14d, then switch to light Cultivate under conditions for 14-16 days; finally, the induced adventitious buds are cultured for elongation, and then transplanted after rooting culture. Adventitious bud induction rate is as high as 88%. The invention aims at providing a method for efficiently regenerating Jatropha leaf discs for gene improvement and tissue culture of Jatropha curcas. In order to realize the genetic transformation of Jatropha curcas, lay the foundation for rapid propagation, and promote the industrial development of Jatropha curcas.
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Description

technical field

[0001] The invention relates to the technical field of plant tissue culture, in particular to a method for directly regenerating leaf disks of Jatropha curcas. Background technique

[0002] Jatropha curcas (Jatropha curcas.L.) is a deciduous shrub or small tree growing in the tropics and subtropics. The oil content of Jatropha curcas seeds is high, up to 40%-60%, surpassing common oil crops such as rape and soybean, and Jatropha curcas oil can be used in various diesel engines as biodiesel, and is an important energy plant. But at present, Jatropha curcas planting area is narrow, only distributed in tropical and subtropical regions of Asia, Africa and America, and the natural distribution in my country is about 11,000 hm 2 , mainly concentrated in Sichuan, Yunnan, and Guizhou. Moreover, the yield of Jatropha curcas is relatively low. At present, the artificially planted Jatropha curcas forest can only produce 100-300kg fruit per mu, and can process 30-100kg...

Claims

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