A kind of leaf disc direct regeneration method of Jatropha curcas

A technology of jatropha and leaves, applied in the field of direct regeneration of leaf discs of Jatropha curcas, can solve the problems of narrow distribution and low yield, restricting large-scale cultivation and industrialization, and low yield of Jatropha curcas, to alleviate the energy and energy crisis, The effect of easy promotion and fast regeneration

Active Publication Date: 2011-11-30
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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Problems solved by technology

And the yield of Jatropha curcas is low. At present, the artificially planted Jatropha curcas can only produce 100-300kg fruit per mu, and can process 30-100kg biodiesel.
Narrow distribution and low yield limit its large-scale cultivation and industrialization as an energy plant

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  • A kind of leaf disc direct regeneration method of Jatropha curcas
  • A kind of leaf disc direct regeneration method of Jatropha curcas

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Embodiment Construction

[0019] The selection of explants in the following examples and comparative examples, the preparation of the culture medium and the culture method etc. are carried out according to Table 2.

[0020] 1. Jatropha curcas explant selection and surface disinfection treatment:

[0021] Select the annual Jatropha curcas leaves in the greenhouse as explants, sterilize them with 20% NaClO for 20 minutes, and cut them into about 9 mm in size with a sterilized blade 2 .

[0022] 2. Place the explants in the culture medium to directly induce adventitious buds. The steps include:

[0023] A. Preparation of culture medium: the culture medium is MS basic medium, add ingredients according to Table 2, sterilize at 121°C for 15 minutes, add sodium nitroprusside when the medium is cooled to 50°C;

[0024] B. Cultivation method: Inoculate the leaf disc in the culture medium with the proximal end facing upwards, culture in the dark first, and then switch to culture under light conditions, the tem...

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Abstract

The invention discloses a method for directly regenerating leaf discs of Jatropha curcas. It selects annual Jatropha curcas leaves in the greenhouse as explants and inoculates them in the medium to directly induce adventitious buds; the medium is MS basic medium with 30g·L-1 sucrose, 4-8g·L-1 agar, 0.2 -2.0mg·L-1TDZ, 0.1-1.5mg·L-1IBA, 0.05-3mg·L-1BA, 0.5-8mg·L-1 Sodium Nitroprusside; culture method: first cultivate in dark for 10-14d, then switch to light Cultivate under conditions for 14-16 days; finally, the induced adventitious buds are cultured for elongation, and then transplanted after rooting culture. Adventitious bud induction rate is as high as 88%. The invention aims at providing a method for efficiently regenerating Jatropha leaf discs for gene improvement and tissue culture of Jatropha curcas. In order to realize the genetic transformation of Jatropha curcas, lay the foundation for rapid propagation, and promote the industrial development of Jatropha curcas.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for directly regenerating leaf disks of Jatropha curcas. Background technique [0002] Jatropha curcas (Jatropha curcas.L.) is a deciduous shrub or small tree growing in the tropics and subtropics. The oil content of Jatropha curcas seeds is high, up to 40%-60%, surpassing common oil crops such as rape and soybean, and Jatropha curcas oil can be used in various diesel engines as biodiesel, and is an important energy plant. But at present, Jatropha curcas planting area is narrow, only distributed in tropical and subtropical regions of Asia, Africa and America, and the natural distribution in my country is about 11,000 hm 2 , mainly concentrated in Sichuan, Yunnan, and Guizhou. Moreover, the yield of Jatropha curcas is relatively low. At present, the artificially planted Jatropha curcas forest can only produce 100-300kg fruit per mu, and can process 30-100kg...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 陈介南卢孟柱李玲刘伯斌
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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