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Method for preparing low molecular chondroitin sulfate

A technology of chondroitin sulfate and chondroitin sulfate enzyme, which is applied in the biological field and can solve unsolved problems such as production efficiency

Inactive Publication Date: 2010-03-03
徐峥嵘 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the process uses ultrafiltration membranes for molecular weight cut-off, the low-molecular-weight chondroitin sulfate that passes through the membrane is still in an acidic reaction environment and is continuously degraded, which does not solve the problem of production efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take 10g of crude chondroitin sulfate with an average molecular weight of 30000Da, dissolve it in 1000ml of 50mM Tris / HCl and 50mM sodium acetate buffer solution, pH 8.0, add 0.1g of Streptomyces hyaluronidase, keep it warm at 30°C for 20min, then in The ultrafiltration system with a molecular weight cut-off of 3000Da was circulated for 2 hours, and the filtrate was collected for analysis. The average molecular weight of chondroitin sulfate was 3600Da, and the product yield between 3000-5000Da in molecular weight distribution was 8g.

Embodiment 2

[0017] Take 10 g of crude chondroitin sulfate with an average molecular weight of 30,000 Da, dissolve it in 1000 ml of 50 mM Tris / HCl and 50 mM sodium acetate buffer solution, pH 7.5, add 0.2 g of Proteus vulgaris chondroitin sulfate ABC, and incubate at 30°C for 20 min, then Circulate in an ultrafiltration system with a molecular weight cut-off of 3000Da for 2h, collect the filtrate and analyze the average molecular weight of chondroitin sulfate is 4200Da, and the product yield between 3000-5000Da in molecular weight distribution is 7g.

Embodiment 3

[0019] Take 10 g of crude chondroitin sulfate with an average molecular weight of 30,000 Da, dissolve it in 1,000 ml of 50 mM Tris / HCl and 50 mM sodium acetate buffer, pH 8.5, add 0.1 g of heparinase from Flavobacterium heparinus, and incubate at 30°C for 20 min, then in The ultrafiltration system with a molecular weight cut-off of 3000Da was circulated for 2 hours, and the filtrate was collected for analysis. The average molecular weight of chondroitin sulfate was 3800Da, and the product yield between 3000-5000Da in molecular weight distribution was 6g.

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Abstract

The invention relates to a method for producing low molecular weight chondroitin sulfate, which comprises the following steps of: (1) cracking high molecular weight chondrotn sulfate by using acid mucopolysaccharide hydrolase; (2) immediately filtering formed low molecular chondroitin sulfate by using an ultrafiltration membrane with a cut-off molecular weight of 5000 Da to obtain obtain the produced low molecular chondroitin sulfate. The invention has the advantages of high product recovery rate and high product quality.

Description

technical field [0001] The invention relates to a method in the field of biotechnology, in particular to a method for preparing low-molecular chondroitin sulfate. Background technique [0002] Chondroitin sulfate is an acidic mucopolysaccharide substance extracted from animal cartilage. It can increase the activity of lipase and accelerate the decomposition of high triglycerides in chylomicrons into fatty acids to clarify the blood. It also has anti-thrombosis, protects cartilage degradation, and prevents senile joint degeneration. disease, maintain joint health, anti-rheumatism and other effects. Low molecular weight chondroitin sulfate has a better oral absorption rate than natural molecular weight chondroitin sulfate. In the United States, it is not only used as a drug for disease treatment, but also as a high-end nutritional supplement for disease prevention. The consumer group is popular from patients to ordinary people. , the oral dose was increased from 0.8 grams to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14
Inventor 徐峥嵘徐佳珊
Owner 徐峥嵘
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