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Benzazole and methylindol degradation strain LPC24

A technology of LPC24 and LPC24CCTCCNO, which is applied in the field of indole and skatole degradation microorganisms, can solve the problems of degradation rarely seen in reports, and achieve good removal and improved removal efficiency

Inactive Publication Date: 2010-03-10
CHINA UNIV OF GEOSCIENCES (WUHAN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although predecessors have done some research work on the degradation of mixed microorganisms, there are few reports on the degradation of indole or skatole by pure bacteria

Method used

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  • Benzazole and methylindol degradation strain LPC24
  • Benzazole and methylindol degradation strain LPC24
  • Benzazole and methylindol degradation strain LPC24

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] One, a screening method for indole and skatole degrading bacteria:

[0013] (1) Material preparation:

[0014] 1. The experimental wastewater (livestock and poultry breeding wastewater) was taken from the sewage outlet of the original pig farm in Ezhou City, Hubei Province, and the activated sludge was taken from the special wastewater treatment laboratory of the Environmental College of China University of Geosciences (Wuhan). In the anaerobic reactor of wastewater (the experimental wastewater in the anaerobic reactor was taken from the sewage outlet of the original pig farm in Hubei Province, Ezhou City, Hubei Province).

[0015] 2. Culture medium:

[0016] Liquid MSM medium (g / L): (NH 4 ) 2 SO 4 , 0.33; NaNO 3 , 0.85; K 2 HPO 4 , 0.79; KH 2 PO 4 , 0.20; CaCl 2 , 0.05; MgCl 2 , 0.50; FeCl 2 , 0.01, H 2 O 1000mL; pH 7.2; after autoclaving, filter-sterilized skatole and indole were used to make the concentration of skatole and indole in the liquid MSM mediu...

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Abstract

The invention relates to a benzazole and methylindol degradation microorganism, in particular to a benzazole and methylindol degradation strain LPC24, which is characterized by being pseudomonas.putida LPC24 CCTCC NO:M 209099. The colonial morphology is as follows: the bacterial colony is off-white, regularly circular, opaque, viscous, smooth in surface and convex in center as well as neat in edge, and is a sphere bulge; the physiological feature is as follows: the strain is in a straight or slightly-bent bar shape, (0.7-1.1)*(2.0-4.0) micrometers, is arranged in one or pairing way, and is Gram-negative; main biochemical characters are as follows: the strain integrates anaerobic, is chemoheterotrophic bacteria, utilizes nitrate, is positive in oxidase, produces arginine dihydrolase and urease and is movable without producing spores; and the optimum growth temperature is 25-37DEG C. The invention has better capacity of removing benzazole and methylindol in the poultry wastewater.

Description

technical field [0001] The invention relates to an indole and skatole degrading microorganism. Background technique [0002] Indole and skatole are the main heterocyclic aromatic hydrocarbons with malodorous odor in livestock and poultry farm wastewater. They are produced by the decomposition of tryptophan in animals. Both compounds are also found in wastewater from the production of medical supplies, pesticides, disinfectants, and agrochemicals. The large-scale production and wide application of these chemicals have resulted in the pollution of soil, surface water, and groundwater, posing serious threats to human and animal health. Indole and skatole can be absorbed into the blood by the human body and animals, and produce toxic intermediate products after biological activation, which will affect the production of serotonin in the blood, and even cause hemolysis. Studies have shown that the increase of estrone concentration in animals is directly related to the content of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/40
Inventor 李平王焰新王艳红童蕾刘珩刘琨
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)