Medicine carrying system based on nano graphene oxide

A technology of nano-graphite oxide and fossils, which is applied in the direction of drug combination, antineoplastic drugs, and pharmaceutical formulations. Effects of Drug Administration and Targeted Cytotoxicity

Inactive Publication Date: 2010-03-17
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, some factors limit the application of single-walled carbon nanotubes: first, compared with graphene, high-purity single-walled carbon nanotubes are difficult to achieve low-cost large-scale preparation; secondly, the hydrophobicity between single-walled carbon nanotubes The action force is very strong, and it often exists in an aggregated state, so it is difficult to disperse single-walled carbon nanotubes in aqueous solution. In addition, using single-walled carbon nanotubes as drug carriers also involves some complicated chemical modification and purification processes

Method used

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  • Medicine carrying system based on nano graphene oxide
  • Medicine carrying system based on nano graphene oxide
  • Medicine carrying system based on nano graphene oxide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Cell Targeting Experiment

[0020] In this example, the cell experiment was carried out with nano-graphene oxide adsorbed with rhodamine B. Human lung cancer cells (A549) do not express folate receptors, while human breast cancer cells (MCF-7) highly express folate receptors. Both cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum. During the experiment, A549 cells and MCF-7 cells were first seeded in six-well plates, cultured for 24 hours, and then 5-10 μg / ml of nanographene oxide adsorbed with fluorescent dyes was added to each well, and incubated at 37°C for 0.5 -3h. After the incubation, the cells were washed with phosphate buffer to wash away the graphene oxide that did not interact with the cells. Finally, the fluorescence of rhodamine B was observed with a fluorescence microscope. The experimental results showed that the fluorescent signal of MCF-7 cells was significantly stronger than that of A549 cells, indicating that the n...

Embodiment 2

[0021] Example 2: Cell Targeted Toxicity Experiment

[0022] This example takes the targeted cytotoxicity of doxorubicin-loaded nano-graphene oxide as an example. Human lung cancer cells A549 and human breast cancer cells MCF-7 were seeded in 96-well plates at 5,000-10,000 cells / well. After 24 hours of culture, different concentrations of nano-graphene oxide loaded with doxorubicin were added and incubated at 37°C for 3 hours. , and then remove the nano-graphene oxide, add fresh medium and continue to nurture for 24-48h. After the incubation, the cell viability was detected by WST-1 method. The results showed that the nano-graphene oxide linked with folic acid had obvious on-target cytotoxicity (as shown in Figure 4a and Figure 4b).

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Abstract

The invention discloses a medicine carrying system based on nano graphene oxide, and is characterized in that the surface of the nano graphene oxide contains two hydrophilic potential groups of carboxyl and sulfonic acid group, carboxyl of the nano graphene oxide is subject to coupling with targeted molecules of folacin, polypeptide or antibody with the help of chemical cross-linker, and carbon atoms of nano graphene oxide absorb and load with one or more than one medicine molecules containing aromatic nucleus to form a targeted medicine carrying system. Proved by application in laboratories,the medicine carrying system can practically improve targeted medicine application and targeted cytotoxicity on each disease point where the medicine is applied, and provides a new thought for targeted therapy on tumor, cancer and other pathogeny.

Description

technical field [0001] The invention relates to a nano targeted drug carrier, in particular to a targeted drug loading system based on nano graphene oxide. Background technique [0002] Nano-targeted drug carrier is a form of carrier for targeted therapy. The particle size is between a few nanometers and hundreds of nanometers. It uses physical (magnetic field) or biological (ligand receptor recognition, antibody antigen interaction, etc.) The drug carrier gathers at the target site and releases it stably, increasing the drug concentration at the target site and enhancing the therapeutic effect; at the same time reducing the drug distribution at other sites and reducing the side effects of the drug. Targeted drug-loading systems based on nanomaterials generally must be achieved through the following steps: first, surface modification is required to avoid agglomeration of nanomaterials. The most widely used method is to modify nanomaterials with polyethylene glycol (PEG). T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/04A61K47/42A61K47/22A61K45/00A61P35/00
Inventor 张智军夏景光张立明
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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