Method for preparing medicine targeted liposome

A technology targeting liposomes and liposomes, applied in liposome delivery, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the limitations of industrial preparation and application of liposomes, liposomes are easy to polymerize and fusion, unsatisfactory targeting distribution of liposomes, etc., to achieve uniform product particle size distribution, easy industrial automation, and good targeting

Inactive Publication Date: 2003-10-29
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Other preparation methods, such as injection method, vaporization method, freezing method, spray method, etc., also have the disadvantages of unsatisfactory target distribution of drug carrier liposomes, easy polymerization and fusion of liposomes, and poor stability. Limiting the industrial preparation and application of liposomes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Prepare 0.3% SOD solution: 3g SOD is dissolved in 1000mlpH7.2 phosphate buffer or pure water, and stir evenly;

[0020] (2) Preparation of magnetic SOD solution: 0.5g Fe 3 o 4 Add 3ml of distilled water to the fine powder and stir to form a suspension, pour it into the SOD solution prepared above, and stir to form a uniform solution;

[0021] (3) Mixing of magnetic SOD and lecithin: take 20mg of lecithin and 5mg of cholesterol, add 2ml of n-butanol to dissolve them, then add the above-mentioned magnetic SOD solution, and treat with an ultrasonic field at a current intensity of 0.5A to make it Become a stable W / O emulsion;

[0022] (4) Supercritical swelling and precipitation dehydration of magnetic SOD liposomes: move the above-mentioned W / O emulsion into the high-pressure container of the supercritical device, feed supercritical carbon dioxide, boost the pressure to 7.4MPa, and after 20 minutes at room temperature Release, the organic solvent is taken away by su...

Embodiment 2

[0025] (1) Prepare 2% SOD solution: 20g SOD is dissolved in 1000mlpH7.2 phosphate buffer or pure water, and stir evenly;

[0026] (2) Preparation of magnetic SOD solution: 2g Fe 3 o 4 Add 10ml of distilled water to the fine powder and stir to form a suspension, pour it into the SOD solution prepared above, and stir to form a uniform solution;

[0027] (3) Mixing of magnetic SOD and lecithin: Take 400mg of lecithin and 200mg of cholesterol, add 3ml of n-butanol to dissolve them, then add the above-mentioned magnetic SOD solution, and treat it with an ultrasonic field at a current intensity of 0.5A to make it Become a stable W / O emulsion;

[0028] (4) Supercritical swelling and precipitation dehydration of magnetic SOD liposomes: move the above-mentioned W / O emulsion into a high-pressure container of a supercritical device, feed supercritical carbon dioxide, boost the pressure to 10MPa, and release it after 60 minutes at room temperature , the organic solvent is taken away by...

Embodiment 3

[0031] (1) Prepare 0.5% SOD solution: 5g SOD is dissolved in 1000mlpH7.2 phosphate buffer or pure water, and stir evenly;

[0032] (2) Preparation of magnetic SOD solution: 1g Fe 3 o 4 Add 5ml of distilled water to the fine powder and stir to form a suspension, pour it into the SOD solution prepared above, and stir to form a uniform solution;

[0033] (3) Mixing of magnetic SOD and lecithin: get 20 mg of lecithin and 6.7 mg of cholesterol, add 2 ml of n-butanol to dissolve it, then add the above-mentioned magnetic SOD solution, and process it in an ultrasonic field with a current intensity of 0.5 A. It becomes a stable W / O emulsion;

[0034] (4) Supercritical swelling precipitation dehydration of magnetic SOD liposome: move the above-mentioned W / O type emulsion into the high-pressure container of the supercritical device, feed supercritical carbon dioxide, boost the pressure to 8.5MPa, and after 30 minutes at room temperature Release, the organic solvent is taken away by su...

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Abstract

A process for preparing the medical target lipoid includes providing SOD, dissolving SOD to obtain solution, magnetizing, preparing water-in-oil emulsion, supercritical fluid swelling precipitation, and freeze drying. Its advantages are high coating rate up to 95%, long semi-life of SOD and good target nature.

Description

technical field [0001] The invention relates to a preparation method of drug targeting liposome, which belongs to the technical field of drug preparation. Background technique [0002] Liposomes are ultra-fine particles with a diameter of several microns to several millimeters formed by the directional arrangement of phospholipid bimolecules, and fat-soluble and water-soluble drugs are encapsulated inside and outside the bimolecular layer. Liposomes can make drugs targeted, improve and prolong the curative effect, alleviate toxicity, avoid drug resistance and change the route of administration, etc. In recent years, in order to improve its targeting and stability, some new lipids such as temperature-sensitive liposomes, pH-sensitive liposomes, immunoliposomes, polymeric membrane liposomes, and proliposomes have been used. body. The existing liposome preparation method has ultrasonic method, and this method is to dissolve water-soluble medicine in the phosphate buffer solut...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K38/44
Inventor 孙君社张中义鲁绯马林苏东海
Owner CHINA AGRI UNIV
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