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Method for preparing erythromycin

A technology selected from erythromycin, which is applied in the field of antibiotic fermentation and preparation, and can solve problems such as high cost pressure

Active Publication Date: 2010-03-17
EAST CHINA UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cost of raw materials accounts for an important proportion of the cost of erythromycin fermentation. Soybean oil is an important source of precursor supply in erythromycin fermentation production. With the rapid rise of soybean oil prices, the pressure on the cost of antibiotic fermentation products that use soybean oil as a fermentation carbon source has increased. getting bigger

Method used

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  • Method for preparing erythromycin
  • Method for preparing erythromycin
  • Method for preparing erythromycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Erythromycin Biosynthesis I

[0070] 1 Materials and methods

[0071] 1.1 Materials As shown in Table 1:

[0072] Table 1

[0073] Material

source

Saccharopolyspora erythraea No.8

Yidu East Sunshine Biochemical Pharmaceutical Co., Ltd.

sodium acetate

sodium propionate

Ammonium acetate

sodium succinate

Sodium citrate

propionic acid

soybean oil

[0074] 1.2 Culture method

[0075] Cultivate eight 500mL shake flasks with a liquid volume of 50mL, a rotation speed of 220r / min, and a temperature of 32°C. The shake flasks are cultured for 7 days, and 0.4% of the low-carbon chain compounds in Table 1 and an equivalent amount of soybean oil are added to the basal medium of the shake flasks. for the control group.

[0076] 2 results

[0077] Shake flask screening test for low-carbon chain compounds to replace soybean oil, that is, to select a series of low-carbon ch...

Embodiment 2

[0080] Erythromycin Biosynthesis II

[0081] 1 Materials and methods

[0082] 1.1 Materials As shown in Table 1:

[0083] 1.2 Culture method

[0084] The 50L fermenter adopts a three-stage fermentation method. Pick fresh slant spores and transfer them to a 500mL shake flask containing 50mL seed medium at a temperature of 33°C and a rotation speed of 220r / min. After 44 hours of cultivation, transfer to a secondary 15L seed tank For cultivation, the inoculum size is 5%, and after 29 hours of cultivation under the same conditions, transfer to a 50L fermenter for cultivation, and the inoculum size is 15%. The FUS-50(A) fermentation tank of Shanghai Guoqiang Biochemical Engineering Equipment Co., Ltd. was used for the fermentation test. The fermentation tank can monitor the fermentation process (including parameters such as pH, DO, OUR, and CER) online.

[0085] 1.350L fermenter fed-batch process

[0086] Soybean oil feeding strategy is carried out at a rate of 0.1g / l.h for 6-3...

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Abstract

The invention discloses a method for preparing erythromycin. The method comprises a step of adding 1.8 to 3.6 liters of 5 to 10 weight / volume percent compound represented by a formula I in a fermentation system for culturing the erythromycin producing bacterium to make the compound in the formula I have the concentration of 0.2 to 0.8 weight / volume so as to obtain erythromycin; the formula I is R1-CH2-R2, wherein R1 may be hydrogen, C1-C4 alkyl, C1-C4 substituted alkyl, C1-C4 carboxylic acid or salt, or C1-C4 substituted alkyl carboxylic acid or salt; R2 may be hydrogen or COOM, and M may be hydrogen and / or metal; and the substituted alkyl and the substituted alkyl carboxylic acid comprise one to three substituents of hydroxyl, amino or halogen. The invention also discloses application ofthe compound represented by the formula I.

Description

technical field [0001] The invention relates to the field of antibiotic fermentation preparation, in particular to a fermentation preparation method of erythromycin. Background technique [0002] Erythromycin is an important raw material and intermediate of macrolide antibiotics. In recent years, with the expansion of the clinical application field of erythromycin and represented by azithromycin, roxithromycin and clarithromycin With the emergence of new semi-synthetic erythromycin, the production and sales of erythromycin have become increasingly active. [0003] Erythromycin fermented oil (such as soybean oil) as an auxiliary carbon source contributes to the synthesis of erythromycin A and erythromycin (see Mirjalili N, Zormpaidis V, Leadlay PF. The Effect of rapeseed oiluptake on the production of erythromycin and triketide lactone by Saccharopolyspora erythraea[J].Biotechnol Prog, 1999,15(5):911.), and the activity of methylmalonyl-CoA carboxyltransferase is higher than...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/08C07H1/06C12P19/62
Inventor 储炬邹祥李万钧段洪武庄英萍张嗣良
Owner EAST CHINA UNIV OF SCI & TECH