A method for detecting the single nucleotide polymorphism of cattle prdm16 gene

A technique for PRDM16 and single nucleotide polymorphism, applied in the field of detecting the single nucleotide polymorphism at position 212237 of the PRDM16 gene of cattle

Inactive Publication Date: 2012-02-22
NORTHWEST A & F UNIV
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Problems solved by technology

There is no research on the genetic variation of PRDM16 gene in animals at home and abroad

Method used

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  • A method for detecting the single nucleotide polymorphism of cattle prdm16 gene
  • A method for detecting the single nucleotide polymorphism of cattle prdm16 gene
  • A method for detecting the single nucleotide polymorphism of cattle prdm16 gene

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Embodiment Construction

[0024] The present invention uses the PCR-RFLP method to detect the single nucleotide polymorphism that may result in a change in the conformation of the encoded protein due to the mutation of the missense codon at the 212237 site of the cattle PRDM16 gene, and the correlation analysis between the SNP and the traits shows that the site Point polymorphisms can become molecular breeding markers. The following will further describe the present invention in detail, which is an explanation of the present invention rather than a limitation.

[0025] a. Design of PCR primers for the region containing the ninth exon of the cattle PRDM16 gene

[0026] Taking the bovine (NC_007314.3) sequence published by NCBI as a reference, Primer 5.0 was used to design PCR primers capable of amplifying the region of the ninth exon of the cattle PRDM16 gene. The primer sequences are as follows:

[0027] Upstream primer: cctaccactc cgtgttccc 19;

[0028] Downstream primer: tcggctgcca atgctc 16;

[0...

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Abstract

The invention discloses a method for detecting the single nucleotide polymorphism of the cattle PRDM16 gene. The whole genome DNA of the cattle to be tested including the PRDM16 gene is used as a template, and the primer pair P is used as a primer to amplify the cattle PRDM16 gene by PCR; After digesting the PCR amplification product with the endonuclease MspI, polyacrylamide gel electrophoresis was performed on the amplified fragment after digestion; the single nucleotide polynucleotide at position 212237 of the cattle PRDM16 gene was identified according to the results of polyacrylamide gel electrophoresis. Morphology; since the PRDM16 gene function involves birth weight, daily weight gain, and body weight growth traits, the detection method provided by the present invention has laid a foundation for the establishment of the relationship between the SNP of the PRDM16 gene and the growth traits, so as to be used for the marker assistance of the growth traits of Chinese yellow cattle Selection (MAS) quickly establishes cattle populations with excellent genetic resources.

Description

technical field [0001] The invention belongs to the field of molecular genetics and relates to the detection of gene single nucleotide polymorphism (SNP), in particular to a method for detecting the 212237th SNP of cattle PRDM16 gene. Background technique [0002] Single nucleotide polymorphism (SNP) refers to the polymorphism caused by the substitution of a single nucleotide (A / T / C / G) in the genomic DNA sequence. Therefore, commonly referred to as SNPs include base substitutions, insertions, deletions, and changes in the copy number of repeated sequences. A SNP indicates that there is a nucleotide change at a certain position in the genome, which is mainly caused by the conversion or transversion of a single base; SNPs with conversion mutations account for about 2 / 3, and other SNPs are in similar level. The cytosine of the CpG dinucleotide is the most mutated site in the genome, most of which are methylated and can be spontaneously deaminated to form thymine. [0003] Va...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 陈宏王璟蓝贤勇淮永涛马亮赖新生胡沈荣雷初朝
Owner NORTHWEST A & F UNIV
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