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Protein sequence of GroEL in lactobacillus casei Zhang

A technology of Lactobacillus casei and heat shock protein, which is applied to the protein sequence field of GroEL in Lactobacillus casei Zhang, and can solve problems such as application scope limitation and the like

Inactive Publication Date: 2012-12-26
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it has good functionality, its application range is very limited due to its sensitivity to heat, humidity and other harsh conditions

Method used

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  • Protein sequence of GroEL in lactobacillus casei Zhang
  • Protein sequence of GroEL in lactobacillus casei Zhang
  • Protein sequence of GroEL in lactobacillus casei Zhang

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Cloning and sequencing of the gene encoding the heat shock protein GroEL in Lactobacillus casei Zhang.

[0051] The fragments obtained by the sequencing of the present invention are the complete sequence of the heat shock protein GroEL coding gene after similarity comparison.

[0052] 1. Experimental method

[0053] 1.1 Design and synthesis of PCR primers

[0054] The PCR primers were designed by themselves using Primer 5.0 based on the relevant sequences provided by GenBank (Accession No: FM177140; CP000423), and synthesized by Shanghai Sunny Biotechnology Co., Ltd. The nucleotide sequences of the primer pair are SEQ ID NO: 3 (forward ) and SEQ ID NO:4 (reverse), the sequence information is as follows:

[0055] SEQ ID NO:3 (forward): 5'-TGAAAAGCACACTATGTAA-3';

[0056] SEQ ID NO: 4 (reverse): 5'-GAAGTATGAAGGTCAAGAC-3';

[0057] 1.2 Extraction of Lactobacillus casei DNA.

[0058] Total DNA was extracted according to the QIAGEN bacterial genome extraction kit instru...

Embodiment 2

[0085] Example 2: Sequence information and bioinformatics analysis of the GroEL gene in Lactobacillus casei Zhang.

[0086] The length of the full-length CDS of the new Lactobacillus casei GroEL of the present invention is 1635 bp, and the detailed sequence is shown in SEQ ID NO:1. The amino acid sequence of GroEL in Lactobacillus casei Zhang was deduced according to the full-length CDS, with a total of 387 amino acid residues, see SEQ ID NO:2 for details, online prediction (http: / / www.expasy.ch / tools / pi_too1.html) The results showed that its molecular weight was 57365 Daltons, and its isoelectric point (pI) was 4.89.

[0087] The full-length CDS sequence related to Lactobacillus casei GroEL and its encoded protein were searched for nucleotide and protein similarity in the non-redundant database of GenBank with the BLAST program, and it was found that it was similar to the published sequence of Lactobacillus casei GroEL in the nucleus. Both the nucleotide level and the amino ...

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Abstract

The invention relates to a protein sequence of GroEL in lactobacillus casei Zhang and provides a new nucleotide sequence for coding a lactobacillus casei heat shock protein GroEL. The sequence is a gene correlated with the stress of lactobacillus casei Zhang and has99 percent of similarity with the known nucleotide sequence FM177140 of the lactobacillus casei BL23 and the nucleotide sequence CP000423 of the lactobacillus casei ATCC334. The invention also provides a nucleotide sequence of GroEL gene in the lactobacillus casei Zhang, which is SEQ ID NO:1. The invention also provides an amino acid sequence of the separated lactobacillus casei heat shock protein GroEL, which is SEQ ID NO:2.

Description

【Technical field】 [0001] The invention belongs to the field of genetic engineering, and in particular relates to a protein coding sequence, in particular to a protein sequence of GroEL in Lactobacillus casei Zhang. 【Background technique】 [0002] Probiotics refer to live bacteria that have beneficial effects on the host through ingestion of appropriate amounts, and Lactobacillus is also the main member of this large family. They can not only be processed into various foods to exert beneficial effects through the digestive tract, but also externally applied to the urinary system and skin where inherent flora also exist. Common functions mainly include maintaining normal intestinal flora, fighting infection, alleviating lactose intolerance, lowering cholesterol, suppressing cancer and stimulating the immune system. In recent years, clinical research hotspots have focused on inflammatory gastrointestinal diseases, allergies, surgical infections, antibiotic-associated diarrhea,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/31C12N15/10C07K14/195C12Q1/68
Inventor 张和平孟和张文羿孙志宏
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY