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Automatic tracking method for video microimage cells based on KHM algorithm

An automatic tracking and microscopic image technology, applied in image analysis, image data processing, calculation, etc., to achieve good tracking effect and good clustering performance

Inactive Publication Date: 2010-06-02
XIDIAN UNIV
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Problems solved by technology

[0005] The purpose of the present invention is to address the deficiencies of the existing cell tracking methods, and propose a method for automatic tracking of cells in video microscopic images based on the KHM algorithm, so as to realize automatic tracking of moving cells and improve the handling of special situations in cell movement. ability

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  • Automatic tracking method for video microimage cells based on KHM algorithm
  • Automatic tracking method for video microimage cells based on KHM algorithm
  • Automatic tracking method for video microimage cells based on KHM algorithm

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Embodiment Construction

[0022] refer to figure 1 , the specific implementation process of the present invention is as follows:

[0023] Step 1, preprocessing the cell motion image, using the watershed method to complete the binary segmentation between the cell target and the background and the cell target, and extracting the target cell from the obtained binary image.

[0024] 1a) Using the Otsu method to calculate the image threshold, and thresholding the input image to obtain the initial target and background binary segmentation results;

[0025] 1b) Use detected object contours as internal control markers for watersheds. The method of obtaining the internal control mark is, for the initial target contour detected by the thresholding method, the gray value of the pixel point where the contour is located is assigned as 1, and the gray value of the pixel point outside the contour is assigned as 0, and the obtained binary image is set to The binary image is used as an internal control mark for water...

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Abstract

The invention discloses an automatic tracking method for video microimage cells based on a KHM algorithm, which mainly overcomes the defects that the traditional tracking method has lower automation degree and the accurate tracking can not be carried out when special conditions occur. The automatic tracking method comprises the steps of: 1, completing binarization segmentation of a cell image by using a watershed method, and extracting target cells; 2, establishing a uniform motion power model for each target cell and initializing; 3, clustering a binary image of the next time by using the KHM algorithm to obtain center positions of all cells; 4, determining positions of the target cells at the next time through the association of the target cells, completing the tracking of motor cells; 5, updating state vectors of the target cell models and error covariance matrixes thereof in the step 2; and 6, circulating the step 3 to the step 5 until the last frame image is tracked. The invention has the advantages of accurate determining of center positions of the cells and good tracking effect under special condition, and can be used for medical image processing.

Description

technical field [0001] The invention belongs to the field of digital image processing, and relates to a method for automatic tracking of cells in video microscopic images, which can be used for medical image processing. Background technique [0002] Cell dynamics is an important aspect of many biomedical treatments, which directly leads to the direction of human health. Cell movement analysis is one of the important components in the study of cell dynamics. Analysis of cell movement, usually by tracking target cells, and then qualitatively or quantitatively analyze the attributes of cell movement speed, displacement, trajectory, shape, etc. Effective methods and necessary means of biopharmaceutical research have very important research significance and practical value in biology, pharmacology and pathology. [0003] At present, the domestic method of cell tracking is mainly manual timing detection and recording with the aid of microscopic equipment, which has a low degree ...

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Application Information

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IPC IPC(8): G06T7/20
Inventor 韩红王爽郑堃刘芳李阳阳马文萍尚荣华吴建设
Owner XIDIAN UNIV
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