Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting gypenosides

A technology of total Gynostemma saponins and an extraction method is applied in the extraction field of Gynostemma Gynostemma saponins, can solve the problems of loss of effective components, large solvent consumption, low content and the like, and achieves the effects of reducing water cost, saving cost and high extraction rate

Inactive Publication Date: 2010-06-16
陕西太极华青科技有限公司
View PDF0 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, although the research on Gynostemma pentaphyllum is blooming everywhere, water extraction and alcohol extraction are mainly used to prepare Gynostemma pentaphylla total saponins extract with low purity, and there are also very few manufacturers who use macroporous resins for separation and purification of Gynostemma pentaphylla total saponins
Among them, the water extraction method and alcohol extraction method have the disadvantages of large loss of gypenoside activity, long time consumption, large solvent consumption, easy pollution of the environment, high separation cost and serious loss of active ingredients during the extraction process, and the yield is generally around 0.3%.
And when adopting macroporous adsorption resin to process, because the polarity of the contained saponin of Gynostemma pentaphyllum is big, and be difficult for separating with pigment, make the separation effect of used resin column not good, the total saponin extraction rate of gained is low, and color and luster is not good, and content is not good. High, brings many problems to industrialization to utilize macroporous resin column to separate and purify total saponins of gypenoside

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting gypenosides
  • Method for extracting gypenosides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Take the dried Gynostemma pentaphyllum and grind it into a powder with a particle diameter of 2-4 mm. The powder under this particle size is beneficial to the extraction and filtration separation of total saponins.

[0025] (2) Weigh 100Kg of Gynostemma pentaphyllum powder, put it in an extraction tank, add NaOH solution with pH=9-11, boil and stir for extraction 3 times, 2 hours each time, filter, and combine the filtrates.

[0026] Alkaline water extraction saves the alcohol extraction and concentration process, which not only saves time but also saves the power loss in the concentration process and the reagent loss in the reagent recovery process. At the same time, the extraction rate is as high as 93.2%, and the cost is low. It is easy to realize low-cost large-scale Production. Moreover, the long-term production tracking test shows that the activity of the total gypenosides in the whole extraction process is stable, and the activity of the total gypenosides is...

Embodiment 2

[0039] (1) Take the dried Gynostemma pentaphyllum and grind it into a powder with a particle diameter of 2-4 mm. The powder under this particle size is beneficial to the extraction and filtration separation of total saponins.

[0040] (2) Weigh 100Kg of Gynostemma pentaphyllum powder, put it in an extraction tank, add NaOH solution with pH=9-11, boil and stir for extraction 3 times, 2 hours each time, filter, and combine the filtrates.

[0041] Alkaline water extraction saves the alcohol extraction and concentration process, which not only saves time but also saves the power loss in the concentration process and the reagent loss in the reagent recovery process. At the same time, the extraction rate is as high as 93.2%, and the cost is low. It is easy to realize low-cost large-scale Production. Moreover, the activity of the total gypenosides is stable throughout the extraction process, and the activity of the total gypenosides is not affected by the alkaline environment and th...

Embodiment 3

[0048] (1) Take the dried Gynostemma pentaphyllum and grind it into a powder with a particle diameter of 2-4 mm. The powder under this particle size is beneficial to the extraction and filtration separation of total saponins.

[0049] (2) Weigh 100Kg of Gynostemma pentaphyllum powder, put it in an extraction tank, add NaOH solution with pH=9-11, boil and stir for extraction 3 times, 2 hours each time, filter, and combine the filtrates.

[0050] Alkaline water extraction saves the alcohol extraction and concentration process, which not only saves time but also saves the power loss in the concentration process and the reagent loss in the reagent recovery process. At the same time, the extraction rate is as high as 93.2%, and the cost is low. It is easy to realize low-cost large-scale Production. Moreover, the activity of the total gypenosides is stable throughout the extraction process, and the activity of the total gypenosides is not affected by the alkaline environment and th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for extracting gypenosides, which comprises the following steps: (1) pulverizing fiveleaf gynostemma herb to obtain fiveleaf gynostemma herb powders; (2) adding the fiveleaf gynostemma herb powders obtained in the step (1) into aqueous solution of NaOH for boiling, filtering, and taking filtrate; (3) adsorbing the filtrate obtained in the step (2) by macroporous absorption resin, eluting the macroporous absorption resin by the aqueous solution of NaOH till colorlessness, collecting NaOH eluant, washing the NaOH eluant by purified water till neutrality, finally desorbing the NaOH eluant by ethanol, and collecting ethanol stripping liquid; (4) adsorbing the NaOH eluant by active carbon, and circulating the NaOH eluant in the step (2) after pigment removal; and (5) distilling ethanol liquid obtained in the step (4) under reduced pressure, reclaiming the ethanol, and drying solid to obtain the gypenosides. The method has the advantages of low cost, high efficiency, no pollution and high product purity.

Description

technical field [0001] The invention relates to a method for extracting total gypenosides. Background technique [0002] Gynostemma pentaphyllum is the precious wealth of Chinese traditional medicine, known as "Southern Ginseng" and "Gospel Grass" in Japan. Chinese medicine believes that Gynostemma pentaphyllum has the effects of clearing heat and detoxifying, eliminating phlegm and relieving cough, calming the nerves, nourishing qi and strengthening the body. Gynostemma pentaphyllum contains more than 80 kinds of gypenosides, and 4 kinds of saponins have the same structure as ginsenosides Rb1, Rb3, Rd, and F2 respectively. The content of ginsenosides is 8 times that of ginseng, and 11 kinds of saponins are 3 times that of Korean ginseng. The saponin content is three times that of ginseng, and it is also rich in gynostemma polysaccharides, 18 kinds of amino acids, various trace elements, rich vitamins, fruit acids, etc. Gypenoside has similar effects to ginsenoside, and ha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/424A61P3/06A61P9/10
Inventor 张向军李培帮
Owner 陕西太极华青科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com