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Method for separating and purifying magnolol and honokiol by HP-20 macroporous resin

A technology of HP-20 and honokiol, which is applied in the field of green chemical technology, can solve problems such as insufficient product quality and market competitiveness, low purity and yield, and inconspicuous efficacy, and achieve operational and product safety and environmental protection Friendly, theoretically novel effect

Inactive Publication Date: 2013-03-06
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously above-mentioned relevant magnolol and honokiol extraction, separation and purification method can prepare magnolol and honokiol extract with certain purity and yield, but due to being limited by technical means and using A large number of toxic organic solvents or chemical preparations make the product obviously have defects such as poor safety or low purity and yield, easy moisture absorption, ineffective efficacy, insufficient product quality and market competitiveness, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Select the cleaned HP-20 macroporous adsorption resin as the separation and purification resin of magnolol and honokiol, and dissolve 400 g of the above crude magnolia extract in 300 ml of edible ethanol with a volume concentration of 60%. Magnolia officinalis crude extract solution, after the solution is applied to the column, HP-20 macroporous resin is saturated and stabilized, first use 3BV of water at a flow rate of 2BV / hour to wash away polar impurities and a small amount of pigment, and then use 3BV volume concentration 65% (all the following ethanol concentrations are volume concentrations) Edible ethanol washes away most of the pigments and other impurities at a flow rate of 2BV / hour, and finally 3BV 78% edible ethanol at a flow rate of 2BV / hour and 3BV 87% edible ethanol is eluted in sequence at a flow rate of 2BV / hour, and collected in sections: ① Collect the 2.0~3.0BV part eluted with 78% edible ethanol, and concentrate and recover under reduced pressure at -0....

example 2

[0024] Select the cleaned HP-20 macroporous adsorption resin as the separation and purification resin of magnolol and honokiol, and dissolve 400 g of the above crude magnolia extract in 300 ml of edible ethanol with a volume concentration of 60%. Magnolia officinalis crude extract solution, after the solution is applied to the column, the HP-20 macroporous resin is saturated and stabilized, first use 3BV of water at a flow rate of 2BV / hour to wash away polar impurities and a small part of pigment, and then use 65% of 3BV Edible ethanol washes away most of the pigments and other impurities at a flow rate of 2BV / hour, and finally 3BV 76% edible ethanol at a flow rate of 2BV / hour and 3BV 85% edible ethanol at a flow rate of 2BV / hour for elution. , Partial collection: ① Collect the 2.0-3.0BV part eluted with 76% edible ethanol, and under the conditions of -0.09MPa and 42℃, the ethanol is recovered by concentration under reduced pressure, and then vacuum freeze-dried into the macropo...

example 3

[0026] The cleaned HP-20 macroporous adsorption resin was selected as the separation and purification resin of magnolol and honokiol, and 400 g of the crude magnolia extract was dissolved in 300 ml of edible ethanol with a volume concentration of 60%. Magnolia officinalis crude extract solution, after the solution is applied to the column, the HP-20 macroporous resin is saturated and stabilized, first use 3BV of water at a flow rate of 2BV / hour to wash away polar impurities and a small part of pigment, and then use 65% of 3BV Edible ethanol washes away most of the pigments and other impurities at a flow rate of 2BV / hour. Finally, 3BV 80% edible ethanol at a flow rate of 2BV / hour and 3BV 89% edible ethanol at a flow rate of 2BV / hour are used for elution. , Partial collection: ① Collect the 2.0-3.0BV part eluted with 80% edible ethanol, and under the conditions of -0.05MPa and 70°C, concentrated under reduced pressure to recover the ethanol, and freeze-dried under vacuum to form t...

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PUM

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Abstract

The invention provides a method for separating and purifying magnolol and honokiol by HP-20 macroporous resin. The method comprises a step of extracting coarse extracts of magnolia bark by 50 to 70 volume percent edible ethanol at the temperature of between 80 and 90 DEG C; and the method also comprises a step of separating and purifying the coarse extracts of magnolia bark by the HP-20 macroporous adsorptive resin, in which the coarse extracts of magnolia bark is dissolved in the edible ethanol, the HP-20 macroporous adsorptive resin is used for adsorption until saturated, the coarse extracts of magnolia bark are eluted by water, low-concentration edible ethanol and high-concentration edible ethanol respectively, the eluent is collected in order, the collected eluent is condensed by decompression to recover the ethanol, and then freeze-dried under a vacuum condition to obtain the purified magnolol and honokiol. By adopting the method, the magnolol and honokiol can be safely and effectively separated and purified by industrialized production.

Description

Technical field: [0001] The invention relates to a green chemical process technology for separating and purifying Chinese medicinal active ingredients of Chinese medicinal plants, in particular to a green separation of magnolol and honokiol in magnolia officinalis using HP-20 macroporous adsorption resin Purification method. Background technique: [0002] Magnolia officinalis is the dry bark, branch bark and root bark of Magnolia officinalis Rehd.et Wils. or M.officinalis Rehd.et Wils.var.biloba Rehd.et Wils. It is a commonly used Chinese medicinal material unique to our country and is known as the three medicinal materials. It is one of the four important Chinese medicinal materials planned and managed by the state: musk, licorice, eucommia and magnolia. It is widely distributed in my country's Yunnan, Sichuan, Guizhou, Yunnan, Zhejiang, Fujian, Hunan and other provinces. There are currently dozens of proprietary Chinese medicine formulas using Magnolia officinalis as raw mate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C39/21C07C37/82A61K36/575
Inventor 肖文军傅冬和李园莉
Owner HUNAN AGRICULTURAL UNIV