Method for fast identifying quality of raw milk
A raw milk, high-quality technology, applied in the biological field, can solve problems such as time-consuming, difficult to detect, and inconsistency between turbidity and microbial growth and reproduction
Inactive Publication Date: 2010-06-16
CHINESE ACAD OF INSPECTION & QUARANTINE +1
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Problems solved by technology
However, since the currently used raw milk testing is mainly sensory, specific gravity and acidity measurement, although illegally added, falsified, and antibiotic residues in raw milk contain various nutrients such as protein, fat, and harmful components such as antibiotics, the content of harmful components such as antibiotics is different from that of high-quality raw milk. Far, but it is difficult to detect with these common detection methods
After the melamine incident, high-performance liquid chromatography and other means were used for detection, but due to the need for high-value detection instruments, the detection cost is high, and it cannot be realized in the detection of raw milk
[0004] At present, the growth and reproduction of microorganisms are mainly measured by flat plate coating and turbidity method. Both of them need to be sampled from food, and the former method takes a long time. In the application of food microbiological testing, there are often inconsistencies between turbidity and microbial growth and reproduction
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Embodiment 1
[0031] Put the raw milk into the triangular flask, seal it with 2 layers of kraft paper, boil it on the electric stove, take it out, cool it in water, boil it again, boil it after cooling, and remove it for later use. In the ultra-clean workbench, take 40-60 μl of raw milk samples from the Erlenmeyer flask to the microwells of a sterile, transparent, flat-bottomed, cell culture microplate with 210-230 μl of brain heart infusion, and then add 10 μl of color-developing Substrate (red tetrazolium, TTC), 20 μl of Streptococcus thermophilic lactis suspension. The microbial suspension is frozen in a small tube in an ultra-low temperature refrigerator in advance. When it is to be used, take out one tube and return it to room temperature before use. Chromogenic substrate, configured as a storage solution, sterilized, stored in the refrigerator at 4°C, and diluted ten times with sterilized distilled water before use. In addition to the raw milk sample to be tested, positive control, n...
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Abstract
The invention relates to a determination method for fast identifying the quality of raw milk, which comprises the following steps of killing microorganisms in the raw milk when keeping the conventional quality thereof by carrying out sterilization on the raw milk; respectively adding chromogenic substrate solution and microorganism suspension to a sample to be tested and a reference substance; carrying out real-time scanning determination and determining by using a dynamic determination mode under a certain culture temperature and wavelength and utilizing an enzyme mark instrument; and identifying the quality: making a microorganism growth curve by taking the determination time as an X axis and an absorption value as a Y axis and identifying the quality of a raw milk sample according to the growth curve. By using the identifying method, the quality of milk can be simply and fast identified with high efficiency.
Description
technical field [0001] The invention belongs to the field of biotechnology and relates to a method for identifying the quality of raw milk. Background technique [0002] The problems of illegal addition, falsification, and antibiotic residues in raw milk in my country are very serious. The root cause of melamine poisoning in milk powder is the illegal addition of melamine in raw milk. However, since the currently used raw milk testing is mainly sensory, specific gravity and acidity measurement, although illegally added, falsified, and antibiotic residues in raw milk contain various nutrients such as protein, fat, and harmful components such as antibiotics, the content of harmful components such as antibiotics is different from that of high-quality raw milk. Very far, but it is difficult to detect it with the current common detection methods. After the melamine incident, high-performance liquid chromatography and other means were used for detection, but due to the need for h...
Claims
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IPC IPC(8): C12Q1/02C12R1/46
Inventor 袁飞陈颖徐宝梁赵贵明张菲菲杨海荣赵勇胜
Owner CHINESE ACAD OF INSPECTION & QUARANTINE