Method for quickening colonization of bag-cultured Lypphyllum decastes strain
A technology of lotus leaf pleura and bag planting, applied in botany equipment and methods, fertilizer mixture, horticulture, etc., can solve the problems such as the vitality of bacteria susceptible to infection by miscellaneous bacteria and mites
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Embodiment 1
[0023] A method for accelerating the colonization of bag-grown lotus leaf phyllomycetes strains, comprising reducing the temperature of the inoculation environment to 20°C, avoiding the decline in the vitality of the strains caused by high-temperature burning and baking, and accelerating the colonization of the strains.
[0024] 1. Preparation of cultivating seed culture substrate: use the following raw materials by weight percentage to prepare the original seed culture substrate:
[0026] Brewer's grains 12%
[0027] Rice Bran 13%
[0028] Cornmeal 5%
[0029] Adjust moisture content to 62%
[0030] The pH of the cultivation medium was adjusted to 6.
[0031] 2. When bagging to make bacteria bags, each bag should contain 1Kg of wet material.
[0032] 3. Inoculation: The inoculation process is carried out under a laminar flow hood equipped with high-efficiency filtration. The inoculation ambient temperature is 20°C, and the inoculation amount is ...
Embodiment 2
[0039] A method for accelerating the colonization of bag-grown lotus leaf phyllomycetes strains, comprising reducing the temperature of the inoculation environment to 20°C, avoiding the decline in the vitality of the strains caused by high-temperature burning and baking, and accelerating the colonization of the strains.
[0040] 1. Preparation of cultivating seed culture substrate: use the following raw materials by weight percentage to prepare the original seed culture substrate:
[0042] Brewer's grains 12%
[0043] Rice Bran 13%
[0044] Cornmeal 5%
[0045] Adjust moisture content to 64%
[0046] The pH of the cultivar culture medium was adjusted to 6.5.
[0047] 2. When bagging to make bacteria bags, each bag should contain 1Kg of wet material.
[0048] 3. Inoculation: The inoculation process is carried out under a laminar flow hood equipped with high-efficiency filtration. The inoculation ambient temperature is 20°C, and the inoculation amo...
Embodiment 3
[0055] The difference between this embodiment and Embodiment 1 is that the inoculation process is always carried out in a sterile environment created by the outer flame of the alcohol lamp. All the other aspects are the same as in Example 1.
[0056] In the present example, 7 groups of lotus-leaf phymejis were co-cultivated, with 100 packs in each group, and the colonization period of the bacteria was 11 days on average.
[0057] The invention is suitable for factory cultivation of the lotus leaf pleura, and is also suitable for factory cultivation of other edible fungi.
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