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Application of breast milk-derived lactobacillus reuteri in reducing fat and regulating fat metabolism rhythm

A Lactobacillus reuteri, lipid metabolism technology, applied in the field of microbial technology and food science, can solve problems such as side effects, achieve the effect of maintaining interaction, promoting phosphorylation of tight junction proteins, and enhancing mucosal barrier

Active Publication Date: 2019-09-06
FINE HUNAN BIOTECHNOLOGY CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, people often treat dyslipidemia by taking statins. However, this method has certain side effects. When the dose is high, it may lead to liver damage characterized by elevated transaminases and elevated creatine kinase. Therefore, it is urgent to find a new drug or method for the treatment of lipid metabolic rhythm disorders to avoid these problems

Method used

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  • Application of breast milk-derived lactobacillus reuteri in reducing fat and regulating fat metabolism rhythm
  • Application of breast milk-derived lactobacillus reuteri in reducing fat and regulating fat metabolism rhythm
  • Application of breast milk-derived lactobacillus reuteri in reducing fat and regulating fat metabolism rhythm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: Breast milk sample collection

[0056] Specific steps are as follows:

[0057] Select breastfeeding mothers who have been proved to be healthy by physical examination and sign survey, and start to collect breast milk after receiving the informed consent for sample collection; wash hands before collecting breast milk with a breast pump, use a breast shield of appropriate size, and place the nipple on the The central part of the breast shield should be kept airtight, and an appropriate pressure (170-60mmHg) should be selected; the breast milk should be stored in a breast storage bag, and transported to the analysis site within 2 hours on ice.

Embodiment 2

[0058] Example 2: Collection of human milk flora and analysis of Lactobacillus reuteri

[0059] Specific steps are as follows:

[0060] Centrifuge the breast milk at 8000-12000rpm for 15min (4°C), remove the liquid, and collect the solids at the bottom of the centrifuge tube; take part of the solids to extract DNA, and use Lactobacillus reuteri-specific PCR to analyze the presence of Lactobacillus reuteri Condition.

Embodiment 3

[0061] Example 3: IgA-binding bacteria enrichment

[0062] Specific steps are as follows:

[0063] Such as figure 1 , the solid matter obtained by centrifuging breast milk was suspended in peptone buffer, bovine serum albumin was added to block non-specific binding (final concentrations were 10%, 0.05-0.5%), biotin-labeled rabbit anti-human IgA serum was added, and incubated for 15 ~30min, then add streptavidin-modified magnetic beads (addition amount: 0.1-1.0mg / mL), absorb the bacteria with a magnet, wash twice with peptone buffer, and obtain the IgA-binding bacterial population.

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Abstract

The invention discloses an application of breast milk-derived lactobacillus reuteri in reducing fat and regulating fat metabolism rhythm, and belongs to the fields of microbial technology and food science. The lactobacillus reuteri FN041 can prevent and / or treat lipid metabolism rhythm disorder and has the functions particularly embodied in: (1) significantly reducing weight gain under the influence of high fat; (2) significantly reducing abnormal increase of peritesticular adipose tissue indexes under the influence of high fat; (3) significantly reducing hepatic fatty infiltration and abnormal increase of testicular fat cell area under the influence of high fat; (4) significantly decreasing abnormal increase of the content of TG, TC, LDL-C and HDL-C in serum under the influence of high fat; (5) significantly decreasing abnormal increase of the plasma FD4 content and the content of LPS, LBP and TNF-alpha in serum under the influence of high fat; and (6) restoring the content of triglyceride in serum and the rhythm of the expression of living clock genes Clock, Bmal1 and Per2 in liver under the influence of high fat.

Description

technical field [0001] The invention relates to an application of breast milk-derived Lactobacillus reuteri for lipid-lowering and regulation of lipid metabolism rhythm, which belongs to the field of microbial technology and food science. Background technique [0002] Studies have shown that the abundance of more than 15% of the bacteria in the intestines of humans and mammals will fluctuate periodically during the day and night, and these rhythmically changing bacteria mainly belong to Clostridium, Lactobacillus and Bacteroides accounts for about 60% of the total number of gut bacteria. Studies have also shown that the rhythmic changes in bacteria in the intestinal tract of humans and mammals will expose the intestinal epithelial cells to different numbers and types of bacteria at different time periods, and these rhythmically changing bacteria will transmit their bacteria through the intestinal epithelial cells. The metabolites are sent to distant tissues such as the live...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04A23L33/135A61K35/747A61P3/06C12R1/225
CPCC12N1/20C12N1/04A23L33/135A61K35/747A61P3/06A23V2002/00C12R2001/225C12N1/205A23V2400/173A23V2200/30
Inventor 孙进李双祁齐策
Owner FINE HUNAN BIOTECHNOLOGY CO LTD
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