Solid fermentation substrate
A technology of solid fermentation and solid cultivation, which is applied in the direction of organic fertilizers, preparation of organic fertilizers, treatment of biological organic parts, etc., and can solve problems such as low microbial activity, long production cycle, and short duration of high temperature
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[0005] As shown in Figure 1, several bacterial agents of the described Erlenmeyer flask liquid culture and solid culture were dried in a sterile ventilated place. According to the ratio of bacterial species, (w / w) 32% of Bacillus subtilis, 22% of Bacillus licheniformis, 25% of Bacillus megaterium, 7% of Aspergillus oryzae and 14% of Amanita amanitae are mixed to form a solid fermentation base.
[0006] As shown in Figure 1, its production process is: ① Erlenmeyer flask liquid culture: put 200mL beef extract peptone liquid medium in a 500mL Erlenmeyer flask, after sterilization, inoculate Bacillus subtilis and Bacillus licheniformis in 2-ring inclined test tubes respectively , Bacillus megaterium, cultured on a shaker at 35°C for 48 hours to amplify; put 200mL potato sucrose liquid culture medium in a 500mL Erlenmeyer flask, after sterilization, inoculate Aspergillus oryzae and Pyreum arborii in 2-ring inclined test tubes respectively, Cultivate on a shaker at 28°C for 4 days t...
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