Immuno-chromatography detection device and detection method thereof

A technology of immunochromatographic detection and detection band, which is applied in the field of medical detection, can solve the problems of low sensitivity, poor accuracy, and high technical requirements of immune detection equipment, and achieve outstanding social and economic benefits, increased sensitivity, and improved stability Effect

Inactive Publication Date: 2010-07-07
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a high-sensitivity, high-specificity immunochromatography device for the problems of low sensitivity, poor accuracy, and high equipment and technical requirements in the prior art.
[0005]

Method used

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  • Immuno-chromatography detection device and detection method thereof
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  • Immuno-chromatography detection device and detection method thereof

Examples

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Embodiment 1

[0021] Example 1, the immunochromatographic detection method is as follows: the sample swims on the membrane made of fibers, the analyte in the sample first reacts with the marker on the membrane, and then swims to the detection zone and the reactive substance under capillary action. Reaction, color development; the marker is a multifunctional polymer formed by coupling reactive substances and indicator substances to the polymer backbone, and the detection zone is coated or chemically cross-linked with reactive substances coupled to the polymer A reactive polymer formed on the backbone.

[0022] 1. Preparation of multifunctional polymers:

[0023] Such as figure 1 Shown is a flow chart of the preparation process of the multifunctional polymer of the embodiment of the present invention, with antibodies as reactive substances and horseradish peroxidase (HRP) as an example for illustration.

[0024] 1. Preparation of HRP-dextran: firstly, under neutral conditions, the dextran a...

Embodiment 2

[0042] Example 2, Immunochromatographic device: The hepatitis C and HCV infection detection kit is taken as an example below.

[0043] Such as figure 2 As shown, the hepatitis C and HCV infection detection kit includes a membrane base, the membrane base is composed of a lower polyester film 10 and an upper chromatographic membrane 11, and the sample pad 1, A marker pad 2 carrying a marker composed of a reactive substance and an indicator substance, a detection strip 3 coated or chemically cross-linked with a reactive substance, and a water-absorbing pad 4 .

[0044] The marker is a multifunctional polymer formed by coupling HCV antigen fragments or HCV core antibody as a reactive substance and horseradish peroxidase as an indicator substance coupled to the polymer backbone-dextran. There are multiple detection bands coated with different fragments of HCV antigen or HCV core antibody.

[0045] Then, according to the results displayed by the detection band, it can be conclude...

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Abstract

The invention discloses an immuno-chromatography detection device and a detection method thereof, which comprise a membrane matrix. The membrane matrix is provided with a sample pad, a marker pad carrying a marker which consists of reactive substance and indicative substance, a detection belt coated or chemically linked crosswise with the reactive substance and an absorbent pad in turn. The marker is multi-functional polymer formed by the coupling of the reactive substance and the indicative substance on a polymer framework. The detection belt is the reactive substance coated or chemically linked crosswise with the membrane matrix or reactive polymer formed by the coupling of the reactive substance on the polymer framework. The detection method is that the sample swims on a membrane and the object to be tested reacts with the marker on the marker pad through the marker pad, then swims to the detection belt to react with the reactive substance under the capillary action, and is developed or checked out through the indicative substance. The invention provides the immuno-chromatography device with high sensitivity and specificity, and the immuno-chromatography detection method with high sensitivity, quickness and reliability.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and relates to an immunochromatographic detection device and a detection method thereof. Background technique [0002] Immunochromatography (IC) is a rapid immunoassay technique developed in the early 1980s. The principle is that with the help of capillary action, the sample swims on the strip fiber membrane, so that the substance to be tested can be combined with the corresponding ligand preset on the membrane, and the color reaction (or fluorescence or direct use of colored markers) can be performed in a short time. (20min) to get intuitive results. Immunochromatography has different names due to the use of different markers, such as enzyme immunochromatography, latex particle immunochromatography, colloidal gold immunochromatography, colloidal selenium immunochromatography, colloidal iron immunochromatography and so on. Colloidal gold immunochromatography is the most widely used an...

Claims

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Application Information

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IPC IPC(8): G01N33/533G01N33/53
Inventor 买制刚林枫李凌云陈少娟卢海蓉易俊波雷明军王晓红黄德新姜丽华
Owner SHENZHEN UNIV
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