Immuno-chromatography detection device and detection method thereof

A technology of immunochromatographic detection and detection band, which is applied in the field of medical detection, can solve the problems of low sensitivity, poor accuracy, and high technical requirements of immune detection equipment, and achieve outstanding social and economic benefits, increased sensitivity, and improved stability Effect
CN101769919AInactive Publication Date: 2010-07-07SHENZHEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHENZHEN UNIV
Publication Date
2010-07-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an immuno-chromatography detection device and a detection method thereof, which comprise a membrane matrix. The membrane matrix is provided with a sample pad, a marker pad carrying a marker which consists of reactive substance and indicative substance, a detection belt coated or chemically linked crosswise with the reactive substance and an absorbent pad in turn. The marker is multi-functional polymer formed by the coupling of the reactive substance and the indicative substance on a polymer framework. The detection belt is the reactive substance coated or chemically linked crosswise with the membrane matrix or reactive polymer formed by the coupling of the reactive substance on the polymer framework. The detection method is that the sample swims on a membrane and the object to be tested reacts with the marker on the marker pad through the marker pad, then swims to the detection belt to react with the reactive substance under the capillary action, and is developed or checked out through the indicative substance. The invention provides the immuno-chromatography device with high sensitivity and specificity, and the immuno-chromatography detection method with high sensitivity, quickness and reliability.
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Description

technical field

[0001] The invention belongs to the technical field of medical detection, and relates to an immunochromatographic detection device and a detection method thereof. Background technique

[0002] Immunochromatography (IC) is a rapid immunoassay technique developed in the early 1980s. The principle is that with the help of capillary action, the sample swims on the strip fiber membrane, so that the substance to be tested can be combined with the corresponding ligand preset on the membrane, and the color reaction (or fluorescence or direct use of colored markers) can be performed in a short time. (20min) to get intuitive results. Immunochromatography has different names due to the use of different markers, such as enzyme immunochromatography, latex particle immunochromatography, colloidal gold immunochromatography, colloidal selenium immunochromatography, colloidal iron immunochromatography and so on. Colloidal gold immunochromatography is the most widely used an...

Claims

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