Oral biological agent for preventing and curing prawn leukopathia

A technology for prawn white spot disease and biological preparations, applied in the field of oral biological preparations, can solve the problems of insufficient protective dosage and unsatisfactory protective effect of recombinant protein, and achieve the effects of improving immunity and survival rate, low cost and simple production process

Inactive Publication Date: 2010-07-14
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the large-scale aquaculture water environment, the protective effect of recombinant protein is not satisfactory, especially through the oral route, the antigen is degraded in the digestive tract, and the absorption rate is low, resulting in insufficient protective dose.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1. An oral biological agent for preventing and treating white spot disease of prawns, which consists of 5% recombinant Bacillus subtilis expressing VP28, 25% chitosan and 70% cornstarch (through 80-200 mesh sieve). The bacterial content of recombinant Bacillus subtilis expressing VP28 was 10 9 pcs / g.

[0022] The preparation method is as follows: simply stir and mix the above three ingredients.

[0023] Experiment 1. Penaeus vannamei were cultured in cement ponds with temperature control at 25±1°C, and were randomly divided into 4 groups, with 3 replicates in each group, 20 in each replicate, and temporarily raised for one week. The daily feeding amount is 2% of the body weight of the prawns.

[0024] In conventional prawn feed, add the oral biological agent (gained in embodiment 1) that accounts for 0.5% of the total weight of prawn feed to prevent and treat prawn white spot disease to feed Penaeus vannamei, feed continuously for 30 days, and then feed 3 d...

Embodiment 2

[0030] Embodiment 2. An oral biological agent for preventing and treating white spot disease of prawns, which is composed of 10% recombinant Bacillus subtilis expressing VP28, 50% chitosan and 40% cornstarch (through 80-200 mesh sieve). The bacterial content of recombinant Bacillus subtilis expressing VP28 was 10 9 pcs / g.

[0031] Experiment 2,

[0032] In conventional prawn feed, add the oral biological agent (gained in embodiment 2) that accounts for 0.1% of the total weight of prawn feed to prevent and treat prawn white spot disease to feed Penaeus vannamei, feed continuously for 30 days, and then feed 3 days with conventional prawn feed; test group.

[0033] Add 0.06% recombinant Bacillus subtilis expressing VP28 (the effective amount is the same as the sum of the amount of recombinant Bacillus subtilis expressing VP28 and chitosan in the test group) in the conventional prawn feed to feed the prawns. Prawns were continuously fed for 30 days, and then fed with convention...

Embodiment 3

[0038] Embodiment 3. An oral biological preparation for preventing and treating white spot disease of prawns, which consists of 1% recombinant Bacillus subtilis expressing VP28, 5% chitosan and 94% cornstarch (passed through 80-200 mesh sieve). The bacterial content of recombinant Bacillus subtilis expressing VP28 was 10 9 pcs / g.

[0039] Experiment 3,

[0040] In conventional prawn feed, add the oral biological agent (gained in embodiment 3) that accounts for 1% of the total weight of prawn feed to prevent and treat prawn white spot disease to feed Penaeus vannamei, feed continuously for 30 days, and then feed 3 days with conventional prawn feed; test group.

[0041] Add 0.06% recombinant Bacillus subtilis expressing VP28 (the effective amount is the same as the sum of the amount of recombinant Bacillus subtilis expressing VP28 and chitosan in the test group) in the conventional prawn feed to feed the prawns. Prawns were continuously fed for 30 days, and then fed with conv...

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PUM

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Abstract

The invention discloses an oral biological agent for preventing and curing prawn leukopathia. The agent has the following components in percentage by weight: 1-10% of reformed Bacillus subtilis expressing VP28, 5-50% of chitosan and balance of corn starch used as a carrier. The agent has safe use, and can effectively prevent and cure prawn leukopathia syndromes.

Description

technical field [0001] The invention relates to an oral biological preparation, in particular to an oral biological preparation for preventing and treating white spot disease of prawns. Background technique [0002] Shrimp white spot syndrome virus (white spot syndrome virus, WSSV, white spot disease of prawns) causes acute and fatal infectious diseases of prawns worldwide, causing huge economic losses in the prawn farming industry. This situation is particularly serious in my country and has become a It is one of the main diseases threatening the shrimp farming industry in my country. In 1995, the International Office of Epizootics (OIE), the Food and Agriculture Organization of the United Nations (FAO) and the Asia-Pacific Aquaculture Development Network Center (NACA) listed it as one of the aquatic animal viral diseases that need to be reported. Vitiligo syndrome has no effective prevention and treatment measures so far, and it is one of the technical problems that the wo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61P31/20A61K31/722
Inventor 李卫芬傅玲琳林志伟周绪霞沈文英
Owner ZHEJIANG UNIV
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