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Method for preparing high-elasticity support promoting cartilage regeneration in situ

An in-situ regeneration and high elasticity technology, applied in the field of bio-regenerative medicine, can solve the problems that there is no research report on the high-elastic cartilage-promoting in-situ regeneration scaffold, and it is difficult to popularize on a large scale, and achieves excellent mechanical properties and promotes regeneration.

Inactive Publication Date: 2010-07-14
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Since the 1960s, allograft bone and joint transplantation, periosteal cartilage transplantation, pedicled fascia flap transplantation, chondrocyte transplantation and artificial prosthesis transplantation have been carried out to repair and reconstruct articular cartilage. However, the limitations of its source of transplants and multiple surgeries make it difficult to promote it on a large scale.
[0005] In summary, in the reports of the existing literature, there is no relevant research report on the high-elastic cartilage in situ regeneration-promoting scaffold described in the present invention.

Method used

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  • Method for preparing high-elasticity support promoting cartilage regeneration in situ
  • Method for preparing high-elasticity support promoting cartilage regeneration in situ

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Gelatin (Gelatin) and modified chitosan (chitosan) were dissolved in dimethyl sulfoxide (DMSO) at a ratio of 1:1 (wt:wt), and 0.5% (wt) 2- Methyl-2-(4-morpholinyl)-1-[4-(methylthio)phenyl]-1-propanone as photocuring initiator, at 365nm, 10w / cm 2 Irradiate under ultraviolet light for 20 minutes to obtain a gel material. The obtained gel is washed and soaked under the action of deionized water, and the DMSO therein is removed to obtain a porous three-dimensional matrix scaffold. Then the stent is immersed in polyethyleneimine (PEI, 1-5 mg / ml) solution for 5-10 minutes and then washed with deionized water with pH=7.0. BMP-2 and HGF are made into a solution with a concentration of 0.1-10 μg / ml, the scaffold is soaked in the solution for 10-30 minutes for adsorption, and then washed with deionized water with pH=7.0. Then immerse in polyallylamine (PAH, 3mg / ml) and polyacrylic acid (PAA, 3mg / ml) solution in turn, cycle 3-4 times, all use deionized water to elute after each ...

Embodiment 2

[0040] Gelatin (Gelatin) and modified chitosan (chitosan) were dissolved in N-N-dimethylacetamide (DMA) in a ratio of 1:3 (wt:wt), and 1.5% (wt )1-[4-(2-hydroxyethoxy)-phenylene]-2-hydroxyl-2',2'-dimethylethanone as photocuring initiator, at 200nm, 5w / cm 2 Irradiate under ultraviolet light for 15 minutes to obtain a gel material. The obtained gel is washed and soaked under the action of deionized water, and the DMA therein is removed to obtain a porous three-dimensional matrix scaffold. Then the stent is immersed in polyethyleneimine (PEI, 1-5 mg / ml) solution for 5-10 minutes and then washed with deionized water with pH=7.0. Prepare BMP-2 and IGF-I into a solution with a concentration of 0.1-10 μg / ml, soak the scaffold in the solution for 10-20 minutes for adsorption, and then wash with deionized water with pH=7.0. Then immerse in polyallylamine (PAH, 3mg / ml) and polyacrylic acid (PAA, 3mg / ml) solution in turn, cycle 3-4 times, all use deionized water to elute after each dep...

Embodiment 3

[0043] Gelatin (Gelatin) and modified chitosan (chitosan) were dissolved in dimethylformamide (DMF) at a ratio of 5:1 (wt:wt), and 1.0% (wt) 2 -Dimethylamino-2-benzyl-1-[4-(4-morpholinyl)phenyl]-1-butanone as photocuring initiator, at 400nm, 10w / cm 2Irradiate under ultraviolet light for 30 minutes to obtain a gel material. The obtained gel is washed and soaked under the action of deionized water, and the DMF therein is removed to obtain a porous three-dimensional matrix scaffold. Then the stent is immersed in polyethyleneimine (PEI, 1-5 mg / ml) solution for 5-10 minutes and then washed with deionized water with pH=7.0. Prepare BMP-2, IGF-I and IGFBP-3 into a solution with a concentration of 0.1-10 μg / ml, soak the scaffold in the solution for 10-20 minutes for adsorption, and then wash with deionized water with pH=7.0. Then immerse in polyallylamine (PAH, 3mg / ml) and polyacrylic acid (PAA, 3mg / ml) solution in turn, cycle 3-4 times, all use deionized water to elute after each d...

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Abstract

The invention belongs to the field of biological regeneration medicine, in particular to a method for preparing a high-elasticity support promoting cartilage regeneration in situ. The method uses gelatin and modified chitosan as main raw materials to prepare a high-elasticity porous three-dimensional substrate material by photocuring and phase separation technologies, and adopts supermolecule layer-by-layer nanometer self-assembly technology to accurately control the structure of a self-assembled membrane on nanometer scale, and loads HGF, BMP-2, IGF-I, IGFBP-3 and other growth factors in thesubstrate material. By controlled release on the growth factors, activation on endogenous bone synovium stem cells (SM-MSCs) on the periphery of a cartilage defect position is realized, the stem cells are promoted to migrate, proliferate and differentiate towards the defect position, and finally the induced self assembly in situ for the cartilage defect position can be realized in vivo.

Description

technical field [0001] The invention belongs to the field of bioregenerative medicine, and in particular relates to a preparation method of a high-elastic cartilage-promoting in-situ regeneration scaffold based on gelatin and modified chitosan as raw materials. Background technique [0002] Cartilage defects or lesions caused by trauma or disease are common diseases in clinical orthopedics. Especially articular cartilage, due to the extremely limited self-repair ability of articular cartilage in the body, once it is damaged or diseased, it is difficult to heal itself, and even lead to joint damage, it often requires exogenous repair or endogenous factors to repair [1] . With the development of life science and engineering, tissue engineering emerged as the times require. People use engineering methods and means to construct and regenerate various tissues and organs to replace damaged tissues, and have made great achievements. Constructing tissue-engineered cartilage with b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/22A61L27/20A61L27/56A61L27/54A61L27/50
Inventor 赵鹏文学军时东陆
Owner TONGJI UNIV