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Production method of casein by lipase method

A production method, lipase technology, applied in lipase to hydrolyze the residual fat in casein to achieve the field of casein purification, which can solve the problems of lack of competitiveness and achieve the effect of strong operability and simple process

Inactive Publication Date: 2010-07-14
青海青海湖生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the different levels of fat content in raw materials, this kind of production process produces products with different levels of fat content, which makes the products lack of competitiveness in the market

Method used

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  • Production method of casein by lipase method
  • Production method of casein by lipase method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1. Hydrolysis

[0043] Accurately weigh 1 part of crude casein and add it to a container with a certain volume, then add 10 parts of water in turn and raise the temperature to 35°C while stirring, then add 1 part of 50% NaOH solution to adjust the pH value to 9.0, install a condenser, electric stirring, Electric heating mantle, heat up while stirring, when the temperature reaches 50°C, the solution will continue to stir at a constant temperature for 50 minutes until the crude casein is completely dissolved, then add 1KLU / g of fat Lipozyme TL 100L lipase (KLU: kilogram lipase unit), and continue to maintain a constant temperature Stir and hydrolyze for 1.5hr, add 10% CaCl after the fat is completely hydrolyzed 2 10 parts of the solution; after stirring evenly, let it stand for 10 minutes and prepare to filter.

[0044] 2. Filter

[0045] Filter the casein solution hydrolyzed in the first step with an 80-mesh nylon mesh to recover the residue.

[0046] 3. Centrifugal s...

Embodiment 2

[0057] 1. Hydrolysis

[0058] Accurately weigh 1 part of crude casein and add it to a container with a certain volume, then add 15 parts of water successively and raise the temperature to 45°C while stirring, then add 10 parts of 50% NaOH solution to adjust the pH value to 11.0, install a condenser, electric stirring, Electric heating mantle, heat up while stirring, when the temperature reaches 50°C, the solution will continue to stir at a constant temperature for 30 minutes until the crude casein is completely dissolved, then add Lipozyme TL 100L lipase (KLU: kilogram lipase unit) at 5KLU / g fat, and continue to maintain a constant temperature Stir and hydrolyze for 0.5hr, add 50% CaCl after the fat is completely hydrolyzed 2 1 part of the solution; stir well and let it stand for 15 minutes, ready to filter.

[0059] 2. Filter

[0060] Filter the casein solution hydrolyzed in the first step with a 120-mesh nylon mesh to recover the residue.

[0061] 3. Centrifugal separatio...

Embodiment 3

[0072] 1. Hydrolysis

[0073] Accurately weigh 1 part of crude casein and add it to a container with a certain volume, then add 10 parts of water in turn and raise the temperature to 35°C while stirring, then add 1 part of 50% NaOH solution to adjust the pH value to 9.0, install a condenser, electric stirring, Electric heating mantle, heat up while stirring, when the temperature reaches 50°C, the solution will continue to stir at a constant temperature for 50 minutes until the crude casein is completely dissolved, then add 1KLU / g of fat Lipozyme TL 100L lipase (KLU: kilogram lipase unit), and continue to maintain a constant temperature Stir and hydrolyze for 1.5hr, after the fat is completely hydrolyzed, add 10% MgSO 4 10 parts of the solution; after stirring evenly, let it stand for 10 minutes and prepare to filter.

[0074] 2. Filter

[0075] Filter the casein solution hydrolyzed in the first step with an 80-mesh nylon mesh to recover the residue.

[0076] 3. Centrifugal ...

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Abstract

The invention discloses a production method of casein by a lipase method, which mainly uses crude casein (Qula) as raw materials. The method comprises the following steps: adopting lipase and adding 100 L of Lipozyme TL (100 L of Novozymes lipase) with the enzyme content of 2 to 5 KLU / g fat into a solution under the conditions of the casein content between 10 and 15 percent, the temperature between 20 and 50 DEG C and the pH value between 6 and 11; hydrolyzing the fat for 0.5 to 1.5 hours; then, adding calcium chloride (CaCl2) or magnesium sulfate (MgSO4) for precipitation; next, using a slag discharge type separating machine for separation; and obtaining casein products with the fat content lower than or equal to 0.5 percent after acidification, dewatering, pelleting and drying. The invention solves the problem of high fat content in the casein products produced by a mechanical separation method, the process is simple and has strong maneuverability, the fat content can be reduced to lower than 0.5 percent, the scale production in a factory can be realized, and the invention is superior to the traditional process in all aspects such as the dissolving time, the energy consumption, the product quality and the production cost.

Description

technical field [0001] The invention relates to the field of biochemical enzymatic hydrolysis, in particular to a method for purifying casein by using lipase to hydrolyze the residual fat in casein by using milk crude protein (called "Qula" by Tibetan herdsmen) after separating ghee as raw material production method. Background technique [0002] At present, domestic casein series product research fields or manufacturers all use mechanical separation method (dish type separator: using the principle of specific gravity difference) to degrease, separate and purify casein (trade name: casein) to produce casein (trade name: casein). The fat content of produced casein is generally between 2.0 and 2.5%. Due to the limitation of factors such as high fat in raw materials, it is difficult to reduce the fat content of casein products to less than 1.0% by mechanical separation. The level of fat content directly affects the quality grade of casein series products, and the specific proc...

Claims

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Application Information

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IPC IPC(8): C12P21/00C07K1/30C07K1/14
Inventor 赵礼赵德
Owner 青海青海湖生物科技发展有限公司
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