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Method for separating and purifying phellodendrine monomer

A technology for separation and purification of phellodendine, which is applied in the field of separation and purification of phellodendine monomer, can solve the problems of lack of high-purity phellodine monomer compound and cannot control the quality of phellodendine and phellodendron medicinal materials, so as to achieve easy quality control and good reproducibility , highly targeted effect

Inactive Publication Date: 2012-08-08
CHENGDU PUSH BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention is exactly to lack high-purity Phellodendriline monomer compound in the above-mentioned prior art, can't strengthen the quality control to Phellodendron medicinal material by the content determination of Phellodendron, provide a kind of separation and purification method of Phellodendron monomer; The method is easy to operate, short in production cycle, high in separation efficiency, stable in process and low in cost, can realize the high-purity separation and preparation of a large amount of cortex base monomers, and obtain cortex base monomers with a purity greater than 98%

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  • Method for separating and purifying phellodendrine monomer
  • Method for separating and purifying phellodendrine monomer
  • Method for separating and purifying phellodendrine monomer

Examples

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Embodiment 1

[0047] The separation and purification method of phellodine monomer of the present embodiment comprises the following main steps:

[0048] A. Extraction of total alkaloids:

[0049] Get 2Kg of Cortex Phellodendri, cut into filaments with a width of about 3mm, put it into a percolation device, add 10L of hydrochloric acid aqueous solution with a concentration of 10% by volume, soak for 36h, control the flow rate to 4L / h, carry out percolation extraction, and collect the percolation The filtrate was 9.6 L in total, the dregs were discarded, and the collected percolation liquid was concentrated under reduced pressure in a water bath at 65°C until a light yellow powder precipitated to obtain 6 L of the concentrated total alkaloid extract, followed by the following step B.

[0050] In this step, soaking in aqueous hydrochloric acid solution, extracting by percolation, and concentrating under reduced pressure, the obtained total alkaloid extract mainly contains components such as Ph...

Embodiment 2

[0068] The separation and purification method of phellodine monomer of the present embodiment comprises the following main steps:

[0069] A. Extraction of total alkaloids:

[0070] Get 0.5Kg of Cortex Phellodendri, cut into filaments with a width of about 1mm, put it into a percolation device, add 12.5L of hydrochloric acid aqueous solution with a concentration of 1% by volume, soak for 5h, control the flow rate to 1L / h, and carry out percolation extraction. Collect a total of 12 L of percolate, discard the dregs, concentrate the collected percolate in a water bath at 45°C under reduced pressure until a light yellow powder precipitates, and obtain 8.5 L of concentrated total alkaloid extract, and proceed to the following step B.

[0071] In this step, soaking in aqueous hydrochloric acid solution, extracting by percolation, and concentrating under reduced pressure, the obtained total alkaloid extract mainly contains components such as Phellodendron and Berberine;

[0072] B....

Embodiment 3

[0089] The separation and purification method of phellodine monomer of the present embodiment comprises the following main steps:

[0090] A. Extraction of total alkaloids:

[0091] Get 4Kg of Cortex Phellodendri, cut into filaments with a width of about 5mm, put it into a percolation device, add 60L of hydrochloric acid aqueous solution with a concentration of 6% by volume, soak for 24h, control the flow rate to 10L / h, carry out percolation extraction, and collect the percolation The filtrate was 57 L in total, the dregs were discarded, and the collected percolation liquid was concentrated under reduced pressure in a water bath at 65°C until a pale yellow powder was precipitated to obtain 45 L of concentrated total alkaloid extract, and the following step B was carried out.

[0092] In this step, soaking in aqueous hydrochloric acid solution, extracting by percolation, and concentrating under reduced pressure, the obtained total alkaloid extract mainly contains components suc...

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Abstract

The invention discloses a method for separating and purifying phellodendrine monomer, which comprises the following steps of: extracting total alkaloid; removing berberine by means of acid deposition; filtering and further removing; separating phellodendrine monomer through a preparative high-efficiency liquid chromatogram; extracting dichloromethane; and drying to prepare the phellodendrine monomer. The method has simple operation, short preparation period, high separation efficiency, stable technology and low cost, and can separate the large number of phellodendrine monomer with high purityto obtain the phellodendrine monomer, the purity of which is more than 98%.

Description

technical field [0001] The invention relates to the technical field of isolating compound monomers from plants, in particular to a method for separating and purifying phellodine monomers. Background technique [0002] Cortex Phellodendri Chinensis is the dry bark of Phellodendron chinensis Schneid. and P.amurense Rupr. of the Rutaceae plant. Produced in Liaoning, Jilin and Hebei. "Chinese Pharmacopoeia" 2005 edition one contains both of them, which have the effects of clearing away heat and dampness, purging fire and removing steam, detoxifying and treating sores. For damp-heat diarrhea, jaundice, leukorrhea, hot stranguria, athlete's foot, bone steaming labor fever, night sweats, spermatorrhea, sores and swollen toxins. Cortex Phellodendron mainly contains alkaloids such as berberine, jatrorrhizine, cortex cortex, palmatine, and magnolialine; sterols such as 7-dehydrostigmasterol and β-sitosterol; hyperin, dihydrokaempferol Flavonoids and a small amount of volatile oil a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D455/03
Inventor 文焕松郭建华刘丁夏柯
Owner CHENGDU PUSH BIOLOGICAL TECH
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