Method for detecting clonorchis sinensis eggs in gallstone

Abstract

The invention relates to a method for detecting clonorchis sinensis eggs, in particular to a method for detecting clonorchis sinensis eggs in gallstone, comprising the following steps: 1) firstly grinding stones and filtering the ground stones to prepare platelets; 2) observing whether the platelets contain clonorchis sinensis eggs through an optical microscope; 3) observing the platelets or the sectioned stones through a scanning electron microscope if the clonorchis sinensis eggs are not observed; and 4) detecting the clonorchis sinensis eggs by polymerase chain reaction if the clonorchis sinensis eggs are not observed. By detecting the clonorchis sinensis eggs in gallstone, the invention obviously increases the detection rate of infection caused by clonorchis sinensis, provides a new method for clinically preventing and treating infection caused by clonorchis sinensis and exploring the correlation between the gallstone and infection caused by clonorchis sinensis so as to reduce the incidence of the gallstone, improves the precision of diagnosis and achieves the aims of early diagnosis, early treatment and early prevention.

Description

technical field [0001] The invention relates to a method for detecting clonorchis sinensis eggs, in particular to a method for detecting clonorchis sinensis eggs in gallstones. Background technique [0002] Cholelithiasis is one of the common diseases and frequently-occurring diseases of general surgery in the world at present, and the incidence rate in the population is about 10%. Clonorchis sinensis is very widespread in my country, affecting at least 870 million people in China, and the estimated number of infected people is 15 million. It is the most important food-borne parasite endemic to my country. Microscopic examination of Clonorchis sinensis eggs is the main method for the diagnosis of Clonorchis sinensis infection. At present, feces and endoscopy are mainly used to obtain bile-containing intestinal fluid from the duodenum, which is then made into thin slices for ordinary light microscope examination. For Clonorchis sinensis eggs, the results show that the detect...

AI Technical Summary

Problems solved by technology

Microscopic examination of Clonorchis sinensis eggs is the main method for the diagnosis of Clonorchis sinensis infection. At present, feces and endoscopy are mainly used to obtain bile-containing intestinal fluid from the duodenum, which is then made into thin slices for ordinary light microscope examination. For Clonorchis sinensis eggs, the results show that the detection rate of direct stool thin section method is the lowest, and the detection rate of bile sediment microscopy is about 42.6%. The high incidence of stones delays the timing of clinical prophylaxis and treatment of Clonorchis sinensis infection