Method for detecting clonorchis sinensis eggs in gallstone

A technology for Clonorchis sinensis and gallstones, applied in the preparation of test samples, measuring devices, instruments, etc., can solve the problems of missed diagnosis, low detection rate, delaying the timing of Clonorchis sinensis infection, etc., to increase accuracy, The effect of improving the detection rate

Inactive Publication Date: 2010-08-11
广州市番禺区胆囊病研究所
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Problems solved by technology

Microscopic examination of Clonorchis sinensis eggs is the main method for the diagnosis of Clonorchis sinensis infection. At present, feces and endoscopy are mainly used to obtain bile-containing intestinal fluid from the duodenum, which is then made into thin slices for ordinary light microscope examination. For Clonorchis sinensis eggs, the results show that the detection rate of direct stool thin section method is the lowest, and the detection rate of bile sediment microscopy is about 42.6%. The high incidence of stones delays the timing of clinical prophylaxis and treatment of Clonorchis sinensis infection

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Embodiment Construction

[0015] The method for detecting Clonorchis sinensis eggs in gallstones according to the present invention, firstly take 1 to 3 gallbladder stones with a diameter of 0.2 to 2.2 cm, add an appropriate amount of normal saline, grind the stones with a mortar, and then add an appropriate amount of normal saline , greater than or equal to 260 mesh nylon yarn filter. Add a small amount of normal saline to paint a thin slice and mark it for microscopic examination. 2 to 3 stone slices per serving. Then use an optical microscope to scan the entire thin section at a low magnification (at least 100 times), and then use a high-power optical microscope at 400 times or more to observe the fine structure. If Clonorchis sinensis eggs are observed, the following steps are not performed.

[0016] If no Clonorchis sinensis eggs are observed, then observe with a scanning electron microscope. The scanning electron microscope is to observe the suspicious parts in the above-mentioned thin section w...

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Abstract

The invention relates to a method for detecting clonorchis sinensis eggs, in particular to a method for detecting clonorchis sinensis eggs in gallstone, comprising the following steps: 1) firstly grinding stones and filtering the ground stones to prepare platelets; 2) observing whether the platelets contain clonorchis sinensis eggs through an optical microscope; 3) observing the platelets or the sectioned stones through a scanning electron microscope if the clonorchis sinensis eggs are not observed; and 4) detecting the clonorchis sinensis eggs by polymerase chain reaction if the clonorchis sinensis eggs are not observed. By detecting the clonorchis sinensis eggs in gallstone, the invention obviously increases the detection rate of infection caused by clonorchis sinensis, provides a new method for clinically preventing and treating infection caused by clonorchis sinensis and exploring the correlation between the gallstone and infection caused by clonorchis sinensis so as to reduce the incidence of the gallstone, improves the precision of diagnosis and achieves the aims of early diagnosis, early treatment and early prevention.

Description

technical field [0001] The invention relates to a method for detecting clonorchis sinensis eggs, in particular to a method for detecting clonorchis sinensis eggs in gallstones. Background technique [0002] Cholelithiasis is one of the common diseases and frequently-occurring diseases of general surgery in the world at present, and the incidence rate in the population is about 10%. Clonorchis sinensis is very widespread in my country, affecting at least 870 million people in China, and the estimated number of infected people is 15 million. It is the most important food-borne parasite endemic to my country. Microscopic examination of Clonorchis sinensis eggs is the main method for the diagnosis of Clonorchis sinensis infection. At present, feces and endoscopy are mainly used to obtain bile-containing intestinal fluid from the duodenum, which is then made into thin slices for ordinary light microscope examination. For Clonorchis sinensis eggs, the results show that the detect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N23/225G01N1/28C12Q1/68
Inventor 乔铁谢景夏马瑞红蔡洪英
Owner 广州市番禺区胆囊病研究所
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