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Method for specifically and semiquantitatively detecting benzopyrene

A semi-quantitative detection, benzopyrene technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of unfavorable benzopyrene, complicated processing, expensive and other problems, and achieves short detection time, simple sample processing, and rapid detection. Effect

Inactive Publication Date: 2010-08-18
HUNAN UNIV
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  • Abstract
  • Description
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Problems solved by technology

These methods are time-consuming, expensive, and sample pretreatment is complicated, which is not conducive to the rapid and simple detection of benzopyrene

Method used

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  • Method for specifically and semiquantitatively detecting benzopyrene
  • Method for specifically and semiquantitatively detecting benzopyrene
  • Method for specifically and semiquantitatively detecting benzopyrene

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Embodiment Construction

[0020] The following embodiments are intended to further illustrate the present invention, rather than limit the present invention.

[0021] By means of the method of the present invention, the inventors conducted a semi-quantitative determination of benzopyrene in water at a sewer outlet in the Hunan University section of the Xiangjiang River Basin.

[0022] 1. Antigen synthesis

[0023] Use 2 mL of pre-cooled pH 1.5-2.0 hydrochloric acid to prepare a 1-aminopyrene solution with a mass concentration of 10.86 g / L; slowly add an excess of pre-cooled pH 2.0 sodium nitrite solution dropwise to the above solution, and Carry out the diazotization reaction for 1 hour; add the fully reacted 1-aminopyrene diazonium salt solution dropwise into the prepared phosphoric acid solution with a volume of 5 mL, a pH value of 7.5, and a concentration of 0.1 mol / L containing 68 mg of bovine serum albumin. In the buffer solution; use 1mol / L sodium hydroxide solution to adjust the pH value to 7.5...

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Abstract

The invention discloses a method for specifically and semiquantitatively detecting benzopyrene, which comprises the steps of antigen synthesis, antigen fixation, benzopyrene detection and detection signal acquisition. The key points are that a chemiluminescence substrate added with horseradish peroxidase reacts in a darkroom, a signal is developed and exposed by using a photosensitive film and the detection signal is analyzed. In addition, the invention also gropes a condition suitable for detection. Compared with the general method for detecting the benzopyrene, the method has the advantages of simple and convenient process, rapidness, practicability, no need of any instrument, cost saving and accurate result and provides a new path for detecting the benzopyrene in the environment.

Description

technical field [0001] The invention relates to a new method for the simple, rapid and accurate specific semi-quantitative detection of benzopyrene. Background technique [0002] Benzopyrene is a persistent organic pollutant that has long-term persistence, bioaccumulation, semi-volatility and high toxicity, and can migrate long distances through environmental media such as air, water, and organisms, and poses serious hazards to human health and the environment. thing. Realizing the simple, rapid and specific detection of benzopyrene is of great significance for monitoring the pollution degree of benzopyrene in the environment and studying the biological toxicity of benzopyrene in organisms. At present, high-end instruments such as high-performance liquid chromatography, gas chromatography, and fluorescence instrument are mainly used to detect benzopyrene. These methods are time-consuming, expensive, and sample pretreatment is complicated, which is not conducive to the rapi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N21/76
Inventor 罗胜联陈章刘玉堂何晔娟陈玉芳刘承斌蔡青云
Owner HUNAN UNIV
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