Culture medium for producing fusidic acid by fermentation method

A fermentation medium and technology of fusidic acid, which is applied in the field of medium for producing fusidic acid by fermentation, can solve problems such as not given, achieve good utilization effect, increase conversion rate, and reduce cost

Inactive Publication Date: 2010-09-01
JIANGSU JIUYANG BIOLOGICAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And in this paper, although the method of fermentative production of fusidic acid is mentioned, no direct data is given to prove that this method is the optimal culture method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The spore suspension was prepared from the slant of Fusidium coccineum (ATCC 14700), and the inoculum size was 10 6 / mL, put into a 250mL shake flask filled with 50mL seed medium, and shake culture at 26°C for 48 hours to obtain shake flask seeds. The composition of shake flask seed medium is as follows (g / L):

[0018] Glucose 25, soybean powder 3, (NH 4 ) 2 SO 4 3. KH 2 PO 4 0.6, NaCl 0.3, pH 6.0;

[0019] Take 10mL shake flask seeds and insert them into 500mL shake flasks filled with 100mL fermentation medium. The composition of the fermentation medium is as follows (g / L):

[0020] Sucrose 30, glycerin 6.5, cottonseed cake powder 25, soybean powder 2, (NH 4 ) 2 SO 4 2.5, KH 2 PO 4 0.25, MgSO 4 ·7H 2 O 0.025, FeCl 3 ·6H 2 O 0.025, pH 6.8.

[0021] After shaking the fermentation shaker flask at 26°C for 96 hours, the conversion rate of fusidic acid reached 41.9%.

Embodiment 2

[0023] The spore suspension was prepared from the slant of Fusidium coccineum (ATCC 14700), and the inoculum size was 10 6 / mL, put into a 250mL shake flask filled with 50mL seed medium, and shake culture at 26°C for 48 hours to obtain shake flask seeds. The composition of shake flask seed medium is as follows (g / L):

[0024] Glucose 15, soybean powder 4.5, (NH 4 ) 2 SO 4 3.5, KH 2 PO 4 0.5, NaCl 0.2, pH 6.0;

[0025] Take 10mL shake flask seeds and insert them into 500mL shake flasks filled with 100mL fermentation medium. The composition of the fermentation medium is as follows (g / L):

[0026] Sucrose 25, glycerin 6, cottonseed cake powder 15, soybean powder 1.5, (NH 4 ) 2 SO4 2. KH 2 PO 4 0.15, MgSO 4 ·7H 2 O0.02, FeCl 3 ·6H 2 O 0.02, pH 7.2.

[0027] After shaking the fermentation shaker flask at 26°C for 96 hours, the conversion rate of fusidic acid reached 44.1%.

Embodiment 3

[0029] The spore suspension was prepared from the slant of Fusidium coccineum (ATCC 14700), and the inoculum size was 10 6 / mL, put into a 250mL shake flask filled with 50mL seed medium, and shake culture at 26°C for 48 hours to obtain shake flask seeds. The composition of shake flask seed medium is as follows (g / L):

[0030] Glucose 20, soybean powder 5, (NH 4 ) 2 SO 4 3. KH 2 PO 4 0.4, NaCl 0.1, pH 7.0;

[0031] Take 10mL shake flask seeds and insert them into 500mL shake flasks filled with 100mL fermentation medium. The composition of the fermentation medium is as follows (g / L):

[0032] Sucrose 20, glycerin 5, cottonseed cake powder 20, soybean powder 1, (NH 4 ) 2 SO 4 1, KH 2 PO 4 0.2, MgSO 4 ·7H 2 O0.01, FeCl 3 ·6H 2 O 0.01, pH 7.8.

[0033] After shaking the fermentation shaker flask at 26°C for 96 hours, the conversion rate of fusidic acid reached 47.3%.

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PUM

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Abstract

The invention belongs to the field of microbial fermentation engineering, and particularly relates to a culture medium for producing fusidic acid by a fermentation method. The culture medium comprises a seed culture medium and a fermentation culture medium. When cheap cottonseed cake powder and soybean meal are utilized by fusidium coccineum to perform organic nitrogen source fermentation, the fusidic acid fermentation can be stably performed, so the yield is improved, the conversion rate of a final product reaches about 50 percent, and the cost of the culture medium is controlled.

Description

technical field [0001] The invention belongs to the field of microbial fermentation engineering, and in particular relates to a culture medium for producing fusidic acid by fermentation. Background technique [0002] Fusidic acid (Fusidic acid, FA), also known as fusidic acid, is a typical high-efficiency antibiotic. In 1962, the Danish Leo Pharmaceutical Company first reported the discovery and extraction of fusidic acid from the fermentation broth of Fusidium coccineum. It is a narrow-spectrum antibiotic produced by Fusidia spp. It mainly has strong antibacterial activity against Gram-positive bacteria, especially Staphylococcus, including Staphylococcus aureus, coagulase-negative bacteria, Staphylococcus epiphysis, Clostridium Bacillus, coryneform bacteria, etc. have high sensitivity [Collignon P, Turndge J. International journal of antimicrobial agents, 1999, 12: 45-58]. Fusidic acid has been widely used abroad for more than 40 years, but it still maintains high antiba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/00C12R1/645
Inventor 胡燚从蕾蕾纪晓俊李军苏永明
Owner JIANGSU JIUYANG BIOLOGICAL PHARMA
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