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Stable enzymatic peracid generating systems

A technology of peracid and sodium percarbonate, applied in the directions of enzymes, hydrolase, biochemical equipment and methods, etc., can solve problems such as aggravating corrosion of stainless steel containers

Inactive Publication Date: 2010-09-01
DANISCO US INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Additionally, the high ratio of acetic acid to PAA in these commercial solutions exacerbates the problems associated with corrosion of stainless steel vessels during the cleaning process

Method used

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  • Stable enzymatic peracid generating systems
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  • Stable enzymatic peracid generating systems

Examples

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preparation example Construction

[0140] The use of the stable compositions of the present invention for the preparation of aqueous solutions is straightforward: simply add the composition to an appropriate amount of water and stir. Typically, only purified (eg MilliQ water), distilled, sterilized and deionized water is used. Accordingly, the present invention also provides a method of preparing a peracid solution comprising adding the stabilized composition to water and mixing for at least about 30 minutes, 20 minutes, 15 minutes, 10 minutes, 5 minutes or even less minute. In some embodiments, the method of preparation comprises: (a) providing a stable composition comprising: an enzyme having perhydrolase activity (wherein the activity comprises a ratio of perhydrolysis to hydrolysis of at least 2:1); a source of hydrogen oxide and an ester substrate; and (b) adding the composition to water and mixing for at least 20 minutes, thereby producing an aqueous solution of at least about 0.16% by weight peracetic a...

Embodiment 1

[0186] In this example, an experiment was performed to determine peracetic acid (PAA) and PAA combination detergents (commercially available [Dial] used in this example) to the bactericidal curve of Bacillus subtilis (B.subtilis) spores. In these experiments, B. subtilis spores were prepared as known in the art (see, eg, Siccardi et al., J. Bacterid., 121:13-19 [1975]). Assays were performed in duplicate with peracetic acid (32 wt% in acetic acid; Aldrich) in 96-well round bottom microtiter plates (Costar). in 50mM KPO 4 PAA was serially diluted in buffer (pH 7.1) (“buffer”) or in a 1:500 dilution of Purex (stock; Dial) in the same buffer (“buffer+Det”) ( The total volume is 50 μl). The amount of PAA added to the assay was 0, 0.4, 4 or 40 mM. Then a volume of 5 μl of spore suspension (containing 10 9 -10 10 spores) were added to each well and the assay was incubated for 15 minutes at RT. Ice-cold LB (150 μl) (see, for example, Sambrook et al., "Molecular Cloning: A Lab...

Embodiment 2

[0186] In this example, an experiment was performed to determine peracetic acid (PAA) and PAA combination detergents (commercially available [Dial] used in this example) to the bactericidal curve of Bacillus subtilis (B.subtilis) spores. In these experiments, B. subtilis spores were prepared as known in the art (see, eg, Siccardi et al., J. Bacterid., 121:13-19 [1975]). Assays were performed in duplicate with peracetic acid (32 wt% in acetic acid; Aldrich) in 96-well round bottom microtiter plates (Costar). in 50mM KPO 4 PAA was serially diluted in buffer (pH 7.1) (“buffer”) or in a 1:500 dilution of Purex (stock; Dial) in the same buffer (“buffer+Det”) ( The total volume is 50 μl). The amount of PAA added to the assay was 0, 0.4, 4 or 40 mM. Then a volume of 5 μl of spore suspension (containing 10 9 -10 10 spores) were added to each well and the assay was incubated for 15 minutes at RT. Ice-cold LB (150 μl) (see, for example, Sambrook et al., "Molecular Cloning: A Lab...

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Abstract

The present invention provides stable compositions comprising a perhydrolase enzyme, a hydrogen peroxide source, and an ester substrate that efficiently generate aqueous peracid solutions. The generated peracid solutions are suitable for decontaminating and / or sanitizing a wide range of materials and equipment contaminated with pathogens or toxic chemicals. In one preferred embodiment, the stable composition comprises an acyl transferase enzyme, sodium percarbonate, and propylene glycol diacetate, and is stable for 30 days or longer. Upon addition to water, the composition is activated and generates an aqueous solution with a high ratio of peracetic acid to acetic acid.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of US Provisional Application No. 60 / 917,252, filed May 10, 2007, which is hereby incorporated by reference in its entirety. field of invention [0003] The present invention relates to stable compositions comprising a perhydrolase, a source of hydrogen peroxide, and an ester substrate capable of efficiently producing aqueous peracid solutions suitable for decontamination involving a variety of chemical and biological warfare materials and Suitable for general surface cleaning and decontamination. Background of the invention [0004] Peracids are widely accepted as decontamination / disinfectants. Most particularly, peracetic acid (also known as "peracetic acid" or "PAA") is widely used, for example, for sterilizing items used in the food industry and hospitals and for decontaminating biological hazards. PAA can also be used as a bleaching chemical in cleaning compositions and indust...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/16C12N9/50C12N9/52C11D3/386D06L4/40
CPCC12P7/54C11D3/38654C11D17/042C12N9/14C12N9/0065C11D3/38636
Inventor C·C·巴耐特
Owner DANISCO US INC
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