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Preparation of dry powder water soluble extract of ground beetle and application thereof in medicine

A technology of water extraction and soil turtle, which is applied in the field of biomedicine, can solve the problems of weakening the therapeutic effect, reducing the amount of medicine taken, and large dosage, and achieves the effect of safe polypeptide components and safe clinical application

Active Publication Date: 2011-11-16
ZHEJIANG MEDICINE CO LTD XINCHANG PHAMACEUTICAL FACTORY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention is based on the in-hospital preparation of dry ground beetle powder in Jiangxi Provincial Dermatology Specialist Hospital, which shows good curative effect, quick effect, short treatment time and small side effects in the treatment of viral infectious skin diseases such as herpes zoster, chickenpox, herpes simplex and genital herpes. Advantages, aiming at the disadvantage of inconvenience caused by large dosage, the inventor speculates that the wood turtle and wood turtle are based on the protein properties of wood turtle and wood turtle dry powder, the property that protein can be degraded into polypeptides by pepsin after oral administration, and the good water solubility of polypeptides. Direct aqueous extracts and enzymatic hydrolyzates of dry powder have the potential to reduce the dosage without weakening the therapeutic effect

Method used

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  • Preparation of dry powder water soluble extract of ground beetle and application thereof in medicine
  • Preparation of dry powder water soluble extract of ground beetle and application thereof in medicine
  • Preparation of dry powder water soluble extract of ground beetle and application thereof in medicine

Examples

Experimental program
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Effect test

Embodiment 1

[0030] The preparation of embodiment 1 soil beetle water extraction polypeptide coarse powder

[0031] Suspend 15 g of dry wood beetle powder (obtained from grinding dried wood beetle) in 500 ml of distilled water. The resulting suspension was stirred at a constant temperature of 37.5°C for 40 minutes. The suspension was filtered naturally under normal pressure, and the filtrate was freeze-dried to obtain 2.25g (15%) of the ground beetle water-extracted polypeptide coarse powder (W 0 ), stored at 4°C until use.

Embodiment 2

[0032] Example 2 Separation and Purification of Water-extracted Polypeptide Coarse Powder of Wood Beetle

[0033] Sephadex G25 was processed and packed into a column (2.6×60cm), and 0.005mol / L ammonium acetate was filtered through a 0.45nm microporous membrane as a buffer balance. Dissolve 2.25g of ground beetle water-extracted polypeptide coarse powder in 1ml of distilled water, and centrifuge at 10,000 rpm for 10 minutes. The supernatant was applied to a Sephadex G25 chromatography column, eluted with 0.005mol / L ammonium acetate buffer solution, the flow rate was 0.5mL / min, and one tube was collected in 8 minutes. Each tube was measured by a UV spectrophotometer with a wavelength of 280nm, and the data was recorded. Curves were made with Microsoft Excel, fractions were pooled according to peak shape, and freeze-dried. yielded 0.24 g (10.5%) of W I , 0.54g (24%)W II , 0.74g (33%)W III and 0.19g (8.5%) W IV Four kinds of water-extracted peptide components.

[0034] Examp...

Embodiment 5

[0038] Example 5W IV The sequence of the polypeptide in

[0039] Measure W according to the method of Example 4 IV After the HPLC-ESI-MS, select suitable peak to measure ESI-MS, analyze collection of graphs according to complementary principle ( Figure 4 ), the amino acid sequence is as follows: H-Ser-Cys-Leu(Ile)-Ser-Pro-Val-Ser-Leu(Ile)-Thr-Arg-Pro-Trp-OH.

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Abstract

The invention discloses a process for extracting effective parts for treating herpes zoster from ground beetle dry powder, and a series of polypeptide components obtained from the extracting process. The invention also discloses the finger-print, the amino acid sequence, and the application of the polypeptide components (HPLC, Maldi-TOF and HPLC-ESI-MS) in treating herpes zoster. The extracting process in the invention has the steps of isolating water-extract solution of the ground beetle dry powder by Sephadex G25 column chromatography to obtain a series of polypeptide components, and then preparing the isolated polypeptide components by freeze drying to get frozen powder. Observation of the clinical effects proves that the polypeptide components all have good curative effects on the herpes zoster. From acute toxicity and subacute toxicity tests on animals, the fact that the polypeptide components of the invention have no toxicity, and safe components and clinical practice can be proven.

Description

[0001] The application of the present invention is a divisional application of the invention application with the application number "2006101401601", the application date is September 30, 2006, and the invention name is "Preparation and application of the water extract and enzymatic hydrolyzate of dry wood turtle powder" . technical field [0002] The present invention relates to the preparation process of the effective part of Chinese medicinal materials, especially to the preparation process of the effective part of the dry ground turtle powder. The present invention also relates to the related polypeptide isolated from the effective part, its amino acid sequence, its fingerprint and their use in the treatment of herpes zoster The application belongs to the field of biomedicine. Background technique [0003] Shingles is a common skin disorder that not only affects large numbers of people, but can also be fatal in some severe cases. In the early 1980s, Jiangxi Provincial D...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/10A61K35/64
Inventor 彭师奇赵明魏蕾曹连甲邵丹耿承芳叶发舜廖银根
Owner ZHEJIANG MEDICINE CO LTD XINCHANG PHAMACEUTICAL FACTORY