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Method for extracting and separating high-purity wild baicalin from breviscapinun

A technology of baicalin and breviscapine, which is applied in the field of extraction and separation of active components in plants, can solve problems such as resin residue, and achieve the effects of easy control, short time consumption and good reproducibility

Inactive Publication Date: 2010-09-15
CHENGDU PUSH BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method adopts adsorption resin separation, but there is a problem of resin residue in the preparation of injection raw materials by resin method

Method used

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  • Method for extracting and separating high-purity wild baicalin from breviscapinun
  • Method for extracting and separating high-purity wild baicalin from breviscapinun
  • Method for extracting and separating high-purity wild baicalin from breviscapinun

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Embodiment 1

[0031] The method for extracting and separating scutellarin from scutellarin of the present embodiment is carried out according to the following process steps:

[0032] A high-efficiency separation method of high-purity scutellarin is characterized in that it is carried out according to the following process steps:

[0033] (1) Raw material dissolution: take 80% (UV method detection) scutellarin 100g, add 12 times the amount of DMF according to the mass ratio to dissolve, and obtain 1L scutellarin solution;

[0034] (2), high-efficiency preparation liquid phase separation: filter the scutellarin solution obtained in step (1), and obtain the scutellarin preparation solution through the high-efficiency preparation liquid chromatography column separation, and the high-efficiency preparation liquid chromatography column filler is 18 Alkylsilane bonded silica (C 18 ), the column specification is 50cm×10cm, the chromatographic conditions: the detection wavelength is 350nm, the mobi...

Embodiment 2

[0039] The method for extracting and separating scutellarin from scutellarin of the present embodiment is carried out according to the following process steps:

[0040] A high-efficiency separation method of high-purity scutellarin is characterized in that it is carried out according to the following process steps:

[0041] (1) Dissolving raw materials: Take 50 g of 90% (detected by UV method) scutellarin, add 10 times the amount of DMF according to the mass ratio to dissolve, and obtain 500 mL of scutellarin solution;

[0042] (2), high-efficiency preparation liquid phase separation: filter the scutellarin solution obtained in step (1), and obtain the scutellarin preparation solution through the high-efficiency preparation liquid chromatography column separation, and the high-efficiency preparation liquid chromatography column filler is 18 Alkylsilane bonded silica (C 18 ), the column specification is 50cm×8cm, the chromatographic conditions are: the detection wavelength is ...

Embodiment 3

[0047] The method for extracting and separating scutellarin from scutellarin of the present embodiment is carried out according to the following process steps:

[0048] A high-efficiency separation method of high-purity scutellarin is characterized in that it is carried out according to the following process steps:

[0049] (1) Raw material dissolution: take 95% (UV method detection) scutellarin 20g, add 8 times the amount of DMF according to the mass ratio to dissolve, and obtain 1L scutellarin solution;

[0050] (2), high-efficiency preparation liquid phase separation: filter the scutellarin solution obtained in step (1), and obtain the scutellarin preparation solution through the separation of the high-efficiency preparation liquid chromatography column, and the high-efficiency preparation liquid chromatography column filler is 18 Alkylsilane bonded silica (C 18 ), the column specification is 50cm×5cm, the chromatographic conditions are: detection wavelength 350nm, mobile ...

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Abstract

The invention discloses a high-efficiency separation method of high-purity wild baicalin, comprising the following steps of: (1) dissolving raw materials, wherein DMF (Dimethyl Formamide) is used as solvent dissolution-assistant raw materials; and (2) using methanol-DMF-water as a mobile phase, separating by adopting a high-efficiency preparation liquid-phase chromatographic column, recovering the solvents and drying to obtain a baicalin product with the purity greater than 99%. The method has the advantages of simplicity and rapidity, less solvent consumption, high yield, economy and environmental protection, can be used for preparing a great deal of products once and has higher economic value and academic value.

Description

technical field [0001] The invention discloses a method for quickly separating a large amount of high-purity scutellarin from scutellarin, and belongs to the technical field of extraction and separation of effective components in plants. Background technique [0002] Erigeron breviscapus (Vant.) Hand.-Mazz., also known as Erigeron breviscapus (Vant.) Hand.-Mazz. Scutellarin, etc., wherein the content of scutellarin (Scutellarin) is about 80% (HPLC method detection) is its main active ingredient, also known as scutellarin, scutellarin, chemical name 3', 5, 6-tri Hydroxy-7-O-glucuronide, molecular formula C 21 h 18 o 12 , the structural formula is shown in formula I. [0003] Formula I [0004] Breviscapine has a significant clinical effect in the treatment of cardiovascular and cerebrovascular diseases, and has a large demand. It has been developed into various dosage forms such as injections, tablets, and capsules. Because scutellarin has poor water solubility and fa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 夏柯郭建华肖莹莹文焕松刘丁
Owner CHENGDU PUSH BIOLOGICAL TECH
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