Method for producing bottles for testing total number of bacteria in drinking water
A technology for the total number of bacteria and production methods is applied in the direction of microorganism-based methods, biochemical equipment and methods, and specific-purpose bioreactors/fermenters, which can solve problems such as time-consuming, large error in measurement results, and tediousness, and achieve The result is direct and accurate, the production process is simple, and the effect of strong reliability
Inactive Publication Date: 2010-09-29
YANGTZE UNIVERSITY
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Problems solved by technology
At present, the plate colony counting method is generally used to determine the total number of bacteria in drinking water at home and abroad, but the measurement results of this method have large errors, and it is cumbersome and time-consuming.
Therefore, there are obvious defects in the traditional detection technology, and it is urgent to research and develop new methods and new tools for the detection of the total number of bacteria to meet the requirements of actual production
Method used
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[0011] At room temperature (take the preparation of 1L culture medium as an example, and so on), weigh 5.0g of sodium chloride, 5.0g of beef extract, and 10.0g of peptone, dissolve them in distilled water in turn, and add them to a 1L volumetric flask Mix, then use distilled water to adjust the volume to the 1L mark, shake well, and adjust the pH value of the solution to 7.2-7.4 with acid solution (10% HCl) or lye (10% NaOH), that is, to prepare bacteria in drinking water Total culture medium required for test bottles. Clean and dry the 12ml molded cillin bottle, put the prepared culture solution into the bottle with 9.0ml culture solution per bottle with a liquid dispenser, then cover it with A-13 medicinal rubber stopper, and use common antibiotics The aluminum cap of the glass bottle is sealed by a special sealer, and finally it is sterilized in a high-temperature steam sterilizer at 121°C and 1.05Kg / cm 2 Under certain conditions, the test bottle sealed with a cap was subj...
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The invention relates to a method for producing bottles for testing the total number of bacteria in drinking water, and belongs to the technical field of microbiological testing methods. The method is characterized by comprising the following steps of: dissolving 0.5 percent of sodium chloride, 0.5 percent of beef extract and 0.1 percent of peptone in 98.0 percent of distilled water in turn at the normal temperature; adding the solution of the sodium chloride, the solution of the beef extract and the solution of the peptone into a container for mixing; shaking mixed solution uniformly and sufficiently; regulating the pH value of the solution to be between 7.2 and 7.4 by using acid liquor (10 percent HCl) or alkali liquor (10 percent NaOH) so as to prepare required culture solution; filling 9ml of the prepared culture solution into each bottle; capping, sealing and sterilizing bottles at a high temperature; and cooling to obtain the bottles for testing the total number of the bacteria in the drinking water. The bottles for testing the total number of the bacteria in the drinking water have the characteristics of simple production process, high reliability, and economy and practicability. The total number of the bacteria in a to-be-tested water sample of the drinking water can be tested accurately by using the testing bottles; operation and use are simple; and work efficiency is improved.
Description
Technical field: [0001] The invention relates to a production method of a test bottle for the total number of bacteria in drinking water, and belongs to the technical field of applied microorganism detection methods. Background technique: [0002] In the actual work of scientific research and production, it is often necessary to measure the number of microorganisms. The determination of microorganisms in water, air, soil and other environments can monitor and evaluate the degree of pollution; the determination of microorganisms in drinking water can evaluate whether it is up to standard; the determination of the number of microorganisms in oil field sewage injection water is helpful to evaluate the quality of sewage injection water Wait. Therefore, the determination of the number of microorganisms has a very important guiding role and practical significance for scientific research and practical production. [0003] Commonly used methods for microbial determination include ...
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Login to View More IPC IPC(8): C12M1/24C12Q1/06C12R1/01
Inventor 易绍金
Owner YANGTZE UNIVERSITY