Ultrahigh-concentration beer brewing strain and culture medium for screening same
An ultra-high concentration, medium technology, applied in beer brewing, beer fermentation methods, fungi, etc., can solve problems such as difficult flavor, yeast autolysis, and cell morphology and cell activity reduction.
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Examples
Embodiment 1
[0024] Example 1 Breeding of ultra-high concentration yeast.
[0025] Randomly select three beer yeast strains from the brewery as the starting yeast strains to carry out mutagenesis through the above method. These three yeast strains are respectively: YJ, F, 36, and undergo the following selection steps:
[0026] 1. Activation of the starting strain
[0027] The bacteria were inoculated into the wort with the addition amount of 3%, and cultured on a shaker at 25°C for 24 hours until the logarithmic growth phase of the yeast, at which time the bacteria had the best vigor and were suitable for mutagenesis.
[0028] 2 Cell Suspension Preparation
[0029] Centrifuge the activated bacterial liquid at 3500r / min for 10min, collect the bacterial cells, wash once with sterilized normal saline, shake with a vortex mixer for 10min, count the number of bacterial cells with a hemocytometer, and then adjust the cell concentration with normal saline to make it become 10 6 cells / ml of bac...
Embodiment 2
[0052] The fermentation of embodiment 2 ultra-high-strength beer
[0053] Use the above strains 1, 2, A, B to ferment through the following fermentation steps:
[0054] 1) Saccharification, using 60-70% malt and 30-40% rice to make the original wort with a concentration of 16°P, adding syrup to the boiling pot, and adjusting the concentration of the wort to 20°P;
[0055] 2) Fermentation, after the wort is cooled, the oxygenation amount is 10-12ppm, and the inoculum is inoculated, and the inoculation amount is controlled at 1.5-1.8*10 7 per ml, the fermentation temperature is 14°C, and the sugar content of the booster is controlled at 6-7°P;
[0056] 3) Dilution, the dilution ratio of the high-concentration dilution process is 80-100%.
[0057] Fermentation performance index
[0058]
Embodiment 3
[0059] Embodiment 3 brewery implements
[0060] For a 200,000-ton brewery (all producing 12°P beer), if the ultra-high-concentration fermentation technology is adopted and the original wort concentration is 20°P, it can be compared with the traditional 12°P fermentation process without increasing equipment investment. Compared with that, the production capacity is increased by (20-12) / 12×100=75%, which greatly improves the equipment utilization rate and reduces the production cost.
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Description
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Application Information
- IPC
- C12N1/18; C12C11/00; C12C12/00; C12R1/865
- Inventors
- 王德良; 宋绪磊