Probiotics preparation for conditioning infant intestinal tract and preparation method thereof
A technology for probiotic preparations and infants, which is applied in the fields of probiotic drops and its preparation, and probiotic preparations for regulating the intestines of infants and young children, and can solve the problems of destroying the biological activity of probiotics, short shelf life of probiotics, and Reduce the number of bacteria and other problems, achieve the effect of restoring the function of the micro-ecological environment, increasing the blood supply of the intestine, and promoting the baby's development
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Embodiment 1
[0040] Embodiment 1 A kind of probiotic preparation and its preparation method for conditioning the intestinal tract of infants and young children
[0041] This embodiment is a preparation method of a probiotic preparation for regulating the intestinal tract of infants, which is carried out in sequence according to the following steps:
[0042] (11) After cleaning the aseptic liquid preparation tank, remove the water, add 20kg of walnut oil to it {the ratio of linoleic acid to linolenic acid is (5-6): 1}, 0.002kg of isomaltooligosaccharide, and mix The liquid tank is cooled to below 10°C by low-temperature cooling water, and the liquid in the liquid distribution tank is recorded as A1;
[0043] (12), add 10kg lactobacillus probiotic powder and medium-chain triglyceride 0.01kg to A1 according to the speed of 40g / s, after stirring evenly, deaeration stirring under 0.01MPa negative pressure, deaeration stirring speed is 200rpm, to get whole oil anaerobic embedding suspension flo...
Embodiment 2-5
[0047] Example 2-5 Probiotic preparation for regulating the intestinal tract of infants and young children and its preparation method
[0048] The following examples are respectively a probiotic preparation for regulating the intestinal tract of infants and its preparation method. The preparation steps are similar to those in Example 1, except that the corresponding technical parameters in the preparation process are different, as follows.
[0049]
[0050]
[0051] The preparations prepared in the above examples are made into drops, each drop is 0.2-0.25ml.
[0052] The products prepared in the above examples have stable performance, uniform composition, long-term and stable storage time, light taste, natural safety, high survival rate of probiotics, simple preparation method, easy control of the process, low-temperature ingredients and deoxygenation under negative pressure to make the probiotic package Buried in the carrier, it ensures that the activity of the probioti...
Embodiment 6
[0053] Example 6 Environment simulation experiment of probiotic preparations for regulating the intestinal tract of infants and young children
[0054] This embodiment simulates the colonization of the drops prepared by the present invention in the gastrointestinal tract, constipation and diarrhea, at room temperature, and in the feces of healthy subjects, and fully evaluates the preservation status of the number of viable bacteria in the entire shelf life of the test product And the human body colonization situation, the drop provided by embodiment 1 has been carried out following experiment.
[0055] (1) Stress resistance experiment
[0056] 1. Experiments in gastrointestinal environment
[0057] The drops prepared in Example 1 are used to simulate the death of bacterial strains in the gastric environment. The specific steps are as follows. Take 4 Erlenmeyer flasks in the laboratory, respectively put them into and mark the simulated artificial gastric juice with a pH value ...
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