Artificial seed

An artificial seed, the innermost technology, applied in the direction of gardening tools/equipment, gardening, botanical equipment and methods, etc., can solve the problems that cannot become practical artificial seeds, do not germinate, and paraffin wax is not easy to decompose

Inactive Publication Date: 2010-10-20
MORISHITA JINTAN CO LTD
View PDF6 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to this attempt, although the stability of preservation has indeed been improved, the problem is that paraffin or wax is so stable that it is not easy to decompose, so it does not germinate when sown in the soil, and it is very inconvenient to open the seeds one by one when sowing, and cannot be used as a practical method. strong artificial seeds

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Artificial seed
  • Artificial seed
  • Artificial seed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Embodiment 1: the fruit juice beverage containing live bifidobacterium capsule

[0071] Take 2 platinum circles of Bifidobacterium longum JCM70502 colonies, implant them into 100 mL of liquid skim milk medium containing 15% skim milk, 0.4% yeast extract, 3% glucose, and pH6.5, and place them in an anaerobic box at 37°C. (Mitsubishi Gas Chemical Co., Ltd.) placed in a container in an anoxic state for 15 hours, and cultured with slow shaking at 60 rpm. Meanwhile, because the pH value dropped, the pH value was kept at 5.5 with a 5M-NaOH aqueous solution using an automatic pH regulator. The number of bacteria after cultivating for 15 hours was carried out in BL agar medium (manufactured by Nisshui Pharmaceutical Co., Ltd.) for 24 hours anoxic culture, and the colonies were counted, and the obtained value was 2 × 10 9 pieces / ml. The bacterial solution was treated with 0.01% trypsin F (manufactured by Amano Pharmaceutical Co., Ltd.) at 45° C. for 15 hours and then used afte...

Embodiment 2

[0078] Embodiment 2: the vegetable juice that contains live lactic acid bacteria capsule

[0079] Take 1 platinum circle of Lactococcus lactis JCM7638 colony cultured by CMG agar medium (yeast extract 0.5%, polypeptone 0.5%, NaCl 0.5%, glucose 1%, agar 2%), implant skim milk 12%, yeast Extract 0.4%, glucose 3%, pH 6.5 liquid skimmed milk culture medium 20ml, at the temperature of 37 ℃, carry out static culture for 15 hours. The number of bacteria after 15 hours of cultivation is 2×10 9 pieces / ml. Dilute the bacterial solution 500 times with the same skimmed milk medium as the capsule content solution.

[0080] Next, in the same manner as in Example 1, a three-layer structure capsule (average particle diameter: 1.8 mm) containing the content liquid was prepared.

[0081] 100 g of the prepared capsules, 12% aqueous skim milk solution were treated with 0.01% protease P (manufactured by Amano Pharmaceutical Co., Ltd.) at 40° C. for 15 hours, and then put into a skim milk cultur...

Embodiment 3

[0083] Example 3: Healthy drinks containing Korean ginseng cultured cell capsules

[0084] 5 g (wet weight) of Korean ginseng (Panax ginseng C.A.Meyer) cells subcultured in Murashige-Skoog liquid medium (pH 5.6) containing sucrose 3% and indole-3-acetic acid (IAA) 1 mg / l , put it into a 500ml conical beaker, add 100ml of culture medium, shake and culture at 25°C and 120rpm for 2 weeks to obtain free cell populations. Then, the free cell mass was filtered with a nylon mesh of 80 μm mesh, and the filtered fine cell mass was filtered again with a nylon mesh of 20 μm mesh, and the cell mass remaining on the mesh was collected. 5 g (wet weight) of this cell mass was placed in 100 ml of Murashige-Skoog liquid medium having the same composition as above to form a suspension, which was used as the capsule contents.

[0085] When producing the seamless soft capsule, it was carried out in the same manner as in Example 1, except that the cell suspension as the content liquid was shaken ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an artificial seed, which consists of a seamless soft capsule, wherein the seamless soft capsule comprises a structure with three layers or more than three layers of an innermost layer, an inner skin layer and an outer layer, the inner skin layer covers the innermost layer, and the outer layer covers the inner skin layer, wherein the innermost layer contains plant cell tissues capable of being divided, the inner skin layer consists of an inner skin membrane with solidified oil as major ingredients, and in addition, the outer layer is an outer skin membrane with the biodegradation performance. The artificial seed of the invention has high storage stability, and the germination percentage can be maintained at a high level.

Description

[0001] This case is the filing date for June 28, 2001 , the application number is 01823414.3 , the name of the invention is Contains living cells or Tissue Capsules divisional application technical field [0002] The present invention relates to capsules containing living cells or tissues and applications thereof. Specifically, the present invention relates to capsules filled with living cells or tissues of microorganisms, plants or animals, and the cells or tissues can reproduce, food containing the capsules, and good storage stability. Encapsulated artificial seeds of rapidly germinating redifferentiated plant tissue cells. Background technique [0003] Since ancient times, people have eaten fermented foods obtained by acting microorganisms on foods. A representative fermented food is yogurt. Yogurt is obtained by acting on milk with lactic acid bacteria, bifidobacteria, etc. (hereinafter referred to as lactic acid bacteria, etc.). effect. However, the active lac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 浅田雅宣波多野由美釜口良诚春原秀基
Owner MORISHITA JINTAN CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap