Artificial seed
An artificial seed, the innermost technology, applied in the direction of gardening tools/equipment, gardening, botanical equipment and methods, etc., can solve the problems that cannot become practical artificial seeds, do not germinate, and paraffin wax is not easy to decompose
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0070] Embodiment 1: the fruit juice beverage containing live bifidobacterium capsule
[0071] Take 2 platinum circles of Bifidobacterium longum JCM70502 colonies, implant them into 100 mL of liquid skim milk medium containing 15% skim milk, 0.4% yeast extract, 3% glucose, and pH6.5, and place them in an anaerobic box at 37°C. (Mitsubishi Gas Chemical Co., Ltd.) placed in a container in an anoxic state for 15 hours, and cultured with slow shaking at 60 rpm. Meanwhile, because the pH value dropped, the pH value was kept at 5.5 with a 5M-NaOH aqueous solution using an automatic pH regulator. The number of bacteria after cultivating for 15 hours was carried out in BL agar medium (manufactured by Nisshui Pharmaceutical Co., Ltd.) for 24 hours anoxic culture, and the colonies were counted, and the obtained value was 2 × 10 9 pieces / ml. The bacterial solution was treated with 0.01% trypsin F (manufactured by Amano Pharmaceutical Co., Ltd.) at 45° C. for 15 hours and then used afte...
Embodiment 2
[0078] Embodiment 2: the vegetable juice that contains live lactic acid bacteria capsule
[0079] Take 1 platinum circle of Lactococcus lactis JCM7638 colony cultured by CMG agar medium (yeast extract 0.5%, polypeptone 0.5%, NaCl 0.5%, glucose 1%, agar 2%), implant skim milk 12%, yeast Extract 0.4%, glucose 3%, pH 6.5 liquid skimmed milk culture medium 20ml, at the temperature of 37 ℃, carry out static culture for 15 hours. The number of bacteria after 15 hours of cultivation is 2×10 9 pieces / ml. Dilute the bacterial solution 500 times with the same skimmed milk medium as the capsule content solution.
[0080] Next, in the same manner as in Example 1, a three-layer structure capsule (average particle diameter: 1.8 mm) containing the content liquid was prepared.
[0081] 100 g of the prepared capsules, 12% aqueous skim milk solution were treated with 0.01% protease P (manufactured by Amano Pharmaceutical Co., Ltd.) at 40° C. for 15 hours, and then put into a skim milk cultur...
Embodiment 3
[0083] Example 3: Healthy drinks containing Korean ginseng cultured cell capsules
[0084] 5 g (wet weight) of Korean ginseng (Panax ginseng C.A.Meyer) cells subcultured in Murashige-Skoog liquid medium (pH 5.6) containing sucrose 3% and indole-3-acetic acid (IAA) 1 mg / l , put it into a 500ml conical beaker, add 100ml of culture medium, shake and culture at 25°C and 120rpm for 2 weeks to obtain free cell populations. Then, the free cell mass was filtered with a nylon mesh of 80 μm mesh, and the filtered fine cell mass was filtered again with a nylon mesh of 20 μm mesh, and the cell mass remaining on the mesh was collected. 5 g (wet weight) of this cell mass was placed in 100 ml of Murashige-Skoog liquid medium having the same composition as above to form a suspension, which was used as the capsule contents.
[0085] When producing the seamless soft capsule, it was carried out in the same manner as in Example 1, except that the cell suspension as the content liquid was shaken ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com