MUC13 gene determining susceptibility/resistance of piglet F4ac diarrhea and application thereof

A susceptibility and genetic technology, applied in the field of animal genetics and breeding, can solve the problems of no major breakthrough, no polymorphism, and inability to explain the genetic basis of ETCF4ac

Inactive Publication Date: 2010-10-20
JIANGXI AGRICULTURAL UNIVERSITY
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  • Abstract
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Problems solved by technology

The applicant conducted research on the effect of this site in the white Duroc × Erhualian resource family constructed by the applicant, and found that this marker has no polymorphism in the ancestor animals, and the F of the resource family 2 Individuals have a well-separated ETEC F4ac adhesion phenotype, which indicates that the SNP marker is only a molecular marker in linkage disequilibr...

Method used

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  • MUC13 gene determining susceptibility/resistance of piglet F4ac diarrhea and application thereof
  • MUC13 gene determining susceptibility/resistance of piglet F4ac diarrhea and application thereof
  • MUC13 gene determining susceptibility/resistance of piglet F4ac diarrhea and application thereof

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Embodiment

[0066] 144 purebred piglets including Duroc, Landrace and Large White pigs were used as experimental animals. These pigs were purchased from 5 large-scale commercial pig farms. Each pig breed selected at least 3 male lines, and each family selected 3-4 A 1-2 month old individual. After the 144 purebred piglets were slaughtered, the brush border ETEC F4ac adhesion phenotype test of porcine small intestinal epithelial cells was carried out in vitro, and the experimental methods and criteria were as described above.

[0067] The SNPA37617G, SNPA32813G, SNPA42784C, SNPA46531G, and SNP C53019T loci of the MUC13 gene were subjected to SNP genotyping in the above 144 purebred piglets using the above-mentioned SNaPshot method. The amplification primers are shown in Table 4. The correlation statistical analysis was carried out between the genotype of each SNP site and the phenotype of ETEC F4ac adhered to pig intestinal brush border in vitro (see Table 5).

[0068] Table 4 is used to ...

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Abstract

The invention discloses an MUC13 gene determining the susceptibility/resistance of piglet F4ac diarrhea and application thereof in boar genetic improvement, which adopts a large-scale white Dorec*Erhualian resource group and a wide cross group of 15 pig breeds at home and abroad, locks a target gene, i.e. the MUC13 gene coded with an ETEC F4ac receptor through the genome scanning chain positioning, high density SNP multi-point chain fine positioning in a destination field, breakpoint recombination analysis, wide cross group association analysis based on linkage disequilibrium, and separates out and clones the MUC13 gene. Five key mutational sites are identified in the MUC13 gene, and are coseparated with ETEC F4ac in vitro adherent phenotype in 144 purebred Duroc, Landrace and Yorkshire piglets from the core groups of five provinces, wherein the accuracy of judging the strongest relevant label of a resistance/ susceptibility individual can be up to 97% (P=1.59*10-21). The invention provides a novel method for breeding and disease resisting of the piglet ETEC F4ac diarrhea.

Description

technical field [0001] The invention relates to the field of animal genetics and breeding, in particular to a MUC13 gene determining susceptibility / resistance to piglet ETEC F4ac diarrhea and its application in genetic improvement of breeding pigs. Background technique [0002] Piglet diarrhea is a common infectious disease in pig production, which has caused huge economic losses to the world's pig industry. Enterotoxigenic Escherichia coli F4 (Enterotoxigenic Escherichia coli F4, ETEC F4) is the most important pathogen causing diarrheal disease in newborn and pre-weaned piglets. Taking my country and Denmark as examples, about 35% and 40% of piglet diarrhea are caused by ETEC F4 infection respectively. In my country, its fatality rate is between 10%-30%, and even higher than this ratio in some places, resulting in a serious decline in the slaughter rate of pigs, the quality of commercial pigs, and the loss of economic benefits. [0003] The pathogenicity of ETEC F4 depend...

Claims

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Application Information

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IPC IPC(8): C12N15/12C12N15/11C07K14/705C12Q1/68
Inventor 黄路生任军晏学明艾华水张志燕
Owner JIANGXI AGRICULTURAL UNIVERSITY
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