Riemerella anatipestifer detection kit and method based on loop-mediated isothermal amplification technology
A ring-mediated isothermal and Riemerella anatipestifer technology is applied in the direction of microorganism-based methods, biochemical equipment and methods, and microbial measurement/inspection. Achieve the effect of simple and fast operation, strong specificity and low detection cost
Inactive Publication Date: 2010-10-27
CHONGQING ACAD OF ANIMAL SCI +1
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The invention discloses riemerella anatipestifer detection kit and method based on a loop-mediated isothermal amplification technology. Based on six specific regions of a 16S rRNA conserved region of riemerella anatipestifer, two specific primers and two specific outer primers are designed in the kit and can specifically distinguish the riemerella anatipestifer and other serotype salmonellae so as to ensure the high specificity and the reliability of a detection result of loop-mediated isothermal amplification. The invention detects the riemerella anatipestifer on the basis of the loop-mediated isothermal amplification technology, can amplify a target sequence rapidly, efficiently and specifically under the isothermal condition, has simple and convenient operation and does not use expensive instruments and reagents; an amplification product can be developed directly by using a fluorescent dye and a result can be judged with naked eyes; the detection cost is low; and the invention is particularly suitable for small and medium size units and field tests.
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