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Protein

A lipid and high-water egg yolk technology, applied in the direction of peptide source, transferase, bacterial peptide, etc., can solve the problem of adding whey protein

Inactive Publication Date: 2010-10-27
DUPONT NUTRITION BIOSCIENCES APS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0036] However, there are many difficulties in adding whey protein to the production process such as cheese making

Method used

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Examples

Experimental program
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Embodiment 1

[0989] Example 1: Derived from Aeromonas salmonicida subsp. Cloning, sequencing and heterologous expression of the transferase of Salmonicida).

[0990] Strains used:

[0991] Aeromonas salmonicida subsp. salmonicida (ATCC 14174) was obtained from ATCC and grown overnight at 30°C in Luria-Bertani medium (LB). The cells were centrifuged and genomic DNA was isolated using the genomic DNA isolation procedure from Qiagen Ltd. Genomic DNA Buffer Set (Cat. No. 19060), Proteinase K (Cat. No. 19131) and RNAse A (Cat. No. 19101) were obtained from Qiagen. Ltd (Boundary court Gatwick Court, West Sussex, RH10 2AX).

[0992] The host strain BL21(DE3)pLysS (Novagen) was used to produce recombinant Aeromonas enzymes. Competent cells of BL21(DE3)pLysS were used as hosts transformed with the expression vector pet12-AsalGCAT-pSM. Transformants containing the appropriate plasmids were grown on LB agar medium containing 100 μg ampicillin / ml at 37°C.

[0993] Construction of expression ve...

Embodiment 2

[1007] Example 2: Cloning and expression of Aeromonas hydrophila transferase in E. coli

[1008] Aeromonas hydrophila (ATCC #7965) was obtained from ATCC and grown overnight at 30°C in Luria-Bertani medium (LB). Cells were centrifuged and genomic DNA was isolated using the genomic DNA isolation procedure from Qiagen. Ltd. Genomic DNA Buffer Set (Cat. No. 19060), Proteinase K (Cat. No. 19131) and RNAse A (Cat. No. 19101 ) were all from Qiagen Ltd. (Boundary court Gatwick Court, West Sussex, RH10 2AX).

[1009] The host bacterial strain BL21(DE3)pLysS (Novagen) was used to prepare recombinant Aeromonas enzymes. Competent cells of BL21(DE3)pLysS were used as hosts transformed with the expression vector pet12a-A.h.GCAT-pSMa. Transformants containing the appropriate plasmids were grown at 37°C in LB agar medium containing 100-μg ampicillin / ml.

[1010] Construction of expression vector pet12-AsalGCAT-pSMa:

[1011] For all DNA amplification of the transferase gene from Aeromo...

Embodiment 3

[1024] Example 3: Expression of Aeromonas transferase in Bacillus subtilis 163 up to

[1025] Plasmid construction

[1026] Two different B. subtilis expression vectors (pUB 110 & pBE5) were used for heterologous expression of Aeromonas genes in B. subtilis. The pUB 110 vector contains the alpha amylase promoter and the pBE vector contains the P32 promoter as a regulatory region for expression of the fused Aeromonas genes. In pUB 110, the first amino acid of the mature GCAT gene of Aeromonas is fused in frame with the last amino acid of the xylanase signal peptide of Bacillus subtilis, by in-frame fusion at the restriction site Nhe1, before the mature protein is generated two extra amino acids. pBE5 contains a cgtase signal sequence fused at the Nco 1 site, allowing the recombinant protein to be secreted into the culture filter.

[1027] PCR reactions were performed to obtain the Aeromonas gene fused in-frame with the signal sequences of the pUB 110 and pBE5 vectors. PCR...

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Abstract

A lipid acyltransferase is described. The lipid acyltransferase comprises the amino acid sequence shown as SEQ ID No. 90, or an amino acid sequence which has 95% or more identity with SEQ ID No. 90.

Description

[0001] references [0002] Various documents are cited herein ("documents cited herein"). Each document cited herein, and each document cited or referenced in the documents cited herein, is incorporated herein by reference. technical field [0003] The present invention relates to lipid acyltransferases. [0004] The present invention relates to a method for in situ production of emulsifiers in food products using lipid acyltransferases. [0005] The present invention also relates to a method of using a lipid acyltransferase to generate an emulsifier in situ in a food product, wherein the method allows the emulsifier to be produced without increasing or substantially increasing the free fatty acids in the food product. [0006] The present invention also relates to a method of producing at least two emulsifiers in situ in a food product using a lipid acyltransferase. [0007] The present invention also relates to the use of lipid acyltransferases to generate carbohydrate an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/195C12P7/64C12N9/10A21D8/04A23C19/032C12Q1/48
CPCA23C19/0684A23L1/3208C12N9/18C12N9/1029C12Y301/01C12N11/00A21D8/042A23L1/034A23C19/0328A23C9/1216A23L29/06A23L15/25
Inventor 阿尔诺·D·克赖杰苏珊·M·马德里德乔恩·D·米克凯尔森乔恩·B·索伊马克·特纳乔纳森·古德温斯
Owner DUPONT NUTRITION BIOSCIENCES APS
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