Method for quickly extracting genome DNA from blood clot

Abstract

The invention discloses a method for quickly extracting a genome DNA from a blood clot. The method comprises the following steps of: (1) filtering the blood clot at a 2 to 20 Newton pressure and collecting filtrate, wherein the filtering pore size is 40 to 130 meshes; (2) uniformly mixing the filtrate with erythrocyte lysate, standing the mixture for 4 to 6 minutes, centrifuging 9,000 to 13,000 grams of the mixture for 0.5 to 1.5 minutes, and taking a deposit; (3) uniformly mixing the deposit with cell lysate, performing water bath on the mixture for 8 to 12 minutes at the temperature of between 63 and 67 DEG C, centrifuging 9,000 to 13,000 grams of the mixture for 0.5 to 1.5 minutes, and taking supernatant; and (4) uniformly mixing the supernatant with isopropanol, and purifying the mixture by using a DNA combination column to obtain the genome DNA. The method has the following advantages that: the DNA extracting content and the purity are significantly improved; purification steps are greatly simplified, a plenty of time is saved; any toxic solvent is not used to ensure the health and the safety of a worker; and a low-cost disposable article is used to ensure that potential crossed contaminations are avoided while reducing the cost. The method is suitable for popularization and application.

Description

technical field [0001] The present invention relates to a method for rapidly extracting genomic DNA from blood clots. Background technique [0002] The availability of high-quality genomic DNA is very important in human disease diagnosis and genetic analysis research. Human genomic DNA is generally extracted from anticoagulation in the laboratory, while the large amount of blood clots left over after serological testing has not been utilized. [0003] The main reasons for extracting DNA from anticoagulant are as follows: the technology for DNA extraction from anticoagulant is very mature at present; anticoagulation does not require pretreatment, and the extraction can be completed within 1 hour. [0004] Correspondingly, DNA extraction from blood clots has the following problems: the method is cumbersome, time-consuming and inefficient; cumbersome pre-processing (such as breaking the blood clot with tweezers or a scalpel is not only dangerous, but also makes the operator di...

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Problems solved by technology

[0004] Correspondingly, there are the following problems in extracting DNA from blood clots: the method is loaded down with trivial details, time-consuming and inefficiency; Need loaded down with trivial details pretreatment (as using tweezers or scalpel to stir up blood clots, not only operation process is dangerous, also makes operator directly contact blood clot; Grinding blood clots with a grinder requires careful cleaning of the grinding objects, which can easily cause cross-contamination; digestion of blood clots with proteinase K generally takes more than 16 hours); DNA extraction from blood clots requires the use of organic solvents such as phenol and chloroform, which not only reduces extraction efficiency, but also endangers operations personnel health

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