Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting chlamydia pneumoniae by loop-mediated isothermal amplification and detection kit

A Chlamydia pneumoniae, ring-mediated constant temperature technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., to achieve accurate detection, improve detection efficiency, and improve cost performance

Inactive Publication Date: 2012-10-17
ZHUHAI ENCODE MEDICAL ENG
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art, and provide a simple, rapid, and highly efficient loop-mediated constant temperature amplification method for detecting Chlamydia pneumoniae and a detection kit. This method improves the diagnostic efficiency of Chlamydia pneumoniae , to solve the traditional detection method can not meet the development requirements of clinical diagnosis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting chlamydia pneumoniae by loop-mediated isothermal amplification and detection kit
  • Method for detecting chlamydia pneumoniae by loop-mediated isothermal amplification and detection kit
  • Method for detecting chlamydia pneumoniae by loop-mediated isothermal amplification and detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0040] The present invention will be further elaborated below with regard to specific embodiment:

[0041] Using the loop-mediated constant temperature amplification rapid detection kit of the present invention to detect clinical samples, the detection procedure includes the following steps:

[0042] 1. DNA extraction of samples to be tested:

[0043] (1) Take 200 μL of the sample to be tested or 100 μL of bacterial culture solution in a 1.5 mL sterile centrifuge tube, add 600 μL of suspension (15% Chelex100 resin, 100 mmol / L Tris-HCl pH8.0, 0.1 mmol / L EDTA, 0.1 % sodium azide), fully mixed on the shaker for 30 seconds;

[0044] (3) Boil in a boiling water bath for 10 minutes, then cool rapidly on ice;

[0045] (4) Centrifuge at 10,000 rpm for 5 to 10 minutes in a centrifuge, and take the supernatant into a sterile centrifuge tube, which is the template DNA to be tested.

[0046] 2. The ring-mediated constant temperature amplification reaction of Chlamydia pneumoniae:

[0...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting chlamydia pneumoniae by loop-mediated isothermal amplification and a detection kit, having the advantages of simplicity, rapidness and high detection efficiency. The main scheme of the invention comprises the following steps of: designing a primer set sequence; and carrying out a loop-mediated isothermal amplification reaction on a sample to be detected by utilizing the detection kit, wherein detection methods of a loop-mediated isothermal amplification product comprise the following steps that: 10,000g of the product is centrifuged for 5 minutesat room temperature, and if a result is a positive result, white precipitates can be seen at the bottom of a test tube; a color-developing agent is added, and the positive result becomes green; and a1.5 percent agarose gel electrophoresis method is used, and the result shows a ladder electrophoretic band. Any one of the three loop-mediated isothermal amplification product detection methods can be selected for detection. The methods have the characteristics of accurate detection, high sensitivity, strong specificity, simplicity, convenience and high speed, has the detection sensitivity of 100ccu / ml, and can quickly and accurately identify specific target fungi, thereby changing the current situation that a traditional detection method cannot meet the requirement of the clinical detection development.

Description

technical field [0001] The invention relates to a rapid detection technology of bacteria, in particular to a method and a detection kit for loop-mediated constant temperature amplification detection of chlamydia pneumoniae. Background technique [0002] Chlamydia pneumoniae (chlamydia pneumoniae, CPn) is the third type of chlamydia newly named and classified in 1989. It is an important pathogen that causes acute respiratory infections in humans. Inflammation and pharyngitis), and higher antibody levels were also found in patients with cardiovascular disease, asthma, and sarcoidosis. In recent years, it has been found that Cpn is closely related to AS. [0003] At present, the etiological examination of Chlamydia pneumoniae mainly has the following methods. One is to do cell culture from trachea or nasopharynx aspirated. This culture method, because Chlamydia pneumoniae can only grow and reproduce in living cells, the isolation and culture method is complicated, and the time ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/04
Inventor 孙宜峰于丹曾冰冰
Owner ZHUHAI ENCODE MEDICAL ENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com