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Method to recover bioactive compounds

A bioactive compound technology, applied in the field of regeneration of bioactive compounds, can solve problems such as incomplete separation, low purity, and difficult formulation

Inactive Publication Date: 2010-11-17
LANG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Although this method is capable of recovering valuable bioactive compounds from the adsorbent polymer, the bioactive compounds in the eluted fractions may mix, resulting in the interaction between different bioactive compounds, especially limonoid glucoside and Between flavanone glycosides, there is no complete separation
This results in lower purity and makes formulation difficult

Method used

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  • Method to recover bioactive compounds
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  • Method to recover bioactive compounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0148] Example 1 - Separation of Bioactive Compounds from Acrylic Polymer Adsorbents

[0149] A glass column with an internal diameter of 40mm and a height of 540mm, equipped with a PTFE piston, packed with a 350mm high nonionic ethylene glycol cross-linked polymethyl methacrylate adsorbent polymer resin (CAS 25777- 18-5) (Alimentech P495 inert adsorption polymer supplied by the company Bucher Foodtech). The resin has a bed volume of 400 ml with a column interstitial dead volume of 180 ml. Prior to use, the acrylic polymer sorbent is typically washed with 10 bed volumes of water.

[0150] Three 250ml aliquots of OPE (750ml total) were applied on top of the column containing the acrylic polymer sorbent and each aliquot was permeated down through the column at a rate of 4ml / min. At this speed, each aliquot takes approximately one hour to elute through the column. After applying OPE, the column was washed with two 250ml aliquots of water.

[0151] Then, the eluting solvent ...

Embodiment 2

[0180] Example 2 - Separation of biologically active compounds from an anion exchange resin column

[0181] A diameter is 2cm, the column that length is 20cm is filled with 200mm high weak base anion exchange resin (Diaion WA-30 resin provided by Supelco company, its average particle diameter is 0.47mm, and total exchange capacity is 1.5meq / mL). The bed volume of the resin was 50 mL. Before use, the anion exchange resin (WA-30) was successively washed with 0.5M sodium hydroxide solution of 2 times of bed volume (100mL), water of 5 times of bed volume (250mL), 0.5M hydrochloric acid solution of 2 times of bed volume and 5 times the bed volume of water to condition it. The final wash water has a pH of 4.2.

[0182] Fraction 8 and Fraction 9 obtained in Example 1 were processed to further purify the limonoid glucosides in these fractions. These fractions, with an alcohol content of 30% and 40% respectively, contain a higher proportion of limonoid glucosides.

[0183] Frac...

Embodiment 3

[0192] Example 3 - Method for recovery of bioactive compounds from orange peel extract (OPE)

[0193] A method for the isolation of bioactive compounds from orange peel extract (OPE) is described. The method uses the following 4 separation columns:

[0194] Column A: This column is a glass column with an internal diameter of 40 mm and a height of 540 mm fitted with a Teflon stopcock. It was partially filled with 200 mm high acrylic resin (Alimentech P495 inert absorbent polymer supplied by Bucher Foodtech). The resin has a bed volume of 250 mL, with a column interstitial dead volume of 110 mL.

[0195] Column B: This column is a glass column with an internal diameter of 23 mm and a height of 180 mm fitted with a Teflon plunger. It is partially filled with 180mm high polystyrene-divinylbenzene resin (AmberliteXAD-16 provided by Sigma-Aldrich, which has a surface area of ​​800m 2 / g, the average pore diameter is 100 Angstroms). The bed volume of the resin was 50 mL.

[0...

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Abstract

The invention relates to a process for separating bioactive compounds obtained from vegetable materials. The invention also relates to a process for extracting bioactive compounds from vegetable material.

Description

technical field [0001] The present invention relates to a method for isolating biologically active compounds obtained from vegetable and fruit materials. The present invention also relates to a method for extracting bioactive compounds from vegetable and fruit materials. Background technique [0002] Plant and vegetable matter contain compounds that are biologically active in the human body and can provide beneficial physiological effects, including a reduced risk of cancer, heart disease, and arthritis. [0003] Some bioactive compounds can be found in a large number of plant and vegetable raw materials. For example, citrus fruits contain bioactive compounds that can be included in two broad categories: limonoids and flavonoids. [0004] Limonoids are triterpenoids commonly found in citrus fruits. Limonoids can exist in the form of aglycones, or linked to a glucose molecule (glucoside). It was recently discovered that limonoid glucosides have potent anticancer propertie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D15/04B01D15/08
CPCB01J20/285B01D15/363B01D15/1864B01D15/361C07H1/06C07H17/04C07H17/07
Inventor 蒂姆·朗韦恩·辛普金斯
Owner LANG TECH