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Transgenic plants with increased stress tolerance and yield

A technology of transgenic plants and high yield, applied in plant peptides, plant products, genetic engineering, etc., can solve problems such as crop yield limitations

Inactive Publication Date: 2010-11-17
BASF PLANT SCI GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] Crop yields are limited when soil water is depleted or no water is available during dry periods

Method used

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  • Transgenic plants with increased stress tolerance and yield
  • Transgenic plants with increased stress tolerance and yield
  • Transgenic plants with increased stress tolerance and yield

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0512] Characterization of cDNA

[0513] cDNAs are isolated from proprietary libraries of the corresponding plant species using known methods. Sequences were processed and annotated using bioinformatics analysis. The degrees of amino acid identity and similarity of the isolated sequences to their respective closest known public sequences are shown in Tables 2A to 11A, Tables 2B to 19B, Tables 2C to 16C, Tables 2D to 24D and Tables 2E to 4E Medium (using pairwise comparisons: gap penalty: 10; gap extension penalty: 0.1; scoring matrix: blosum62).

[0514] Table 2A

[0515] GM47143343 (SEQ ID NO: 2) compared to known mitogen-activated protein kinases

[0516] public database accession number

species

Sequence identity (%)

AAD32204

Apricot (Prunus armeniaca)

88.60％

NP_179409

Arabidopsis

85.90％

BAA04870

Arabidopsis

85.60％

CAN70944

Grapes (Vitis vinifera)

82.90％

ABO84371

Medicago truncatul...

Embodiment 2

[1009] gene characterization

[1010] Using standard recombination techniques, the lead genes (Lead gene) b1805 (SEQ ID NO: 287), YER015W (SEQ ID NO: 289), b1091 (SEQ ID NO: 317), b0185 (SEQ ID NO: 319), b3256 ( SEQ ID NO:321), b3255 (SEQ ID NO:329), b1095 (SEQ ID NO:335), b1093 (SEQ ID NO:343), slr0886 (SEQ ID NO:345) and slr1364 (SEQ ID NO:397 ). The functionality of each lead gene was predicted by comparing the amino acid sequence of each lead gene to other genes of known function. Homologue cDNAs were isolated from proprietary libraries of the corresponding species using known methods. Analyze, process and annotate sequences using bioinformatics. The degree of amino acid identity of the isolated sequences to their respective closest known public sequences (using pairwise comparisons: gap penalty: 10; gap extension penalty: 0.1; scoring matrix: blosum62) was used for the selection of homologous sequences, as shown in Tables 2F to 11F.

[1011] Form 2F

[1012] b1805 (...

Embodiment 3

[1054] gene characterization

[1055] The sterol pathway genes B0421 (SEQ ID NO:413), YJL167W (SEQ ID NO:415), SQS1 (SEQ ID NO:435) and YGR175C (SEQ ID NO:443) were cloned using standard recombinant techniques. The functionality of each sterol pathway gene was predicted by comparing the amino acid sequence of the gene with other genes of known function. Homologue cDNAs are isolated from proprietary libraries of the corresponding species using known methods. Analyze, process and annotate sequences using bioinformatics. The degree of amino acid identity of the isolated sequences to their respective closest known public sequences is shown in Tables 2G to 5G (using pairwise comparisons: gap penalty: 11; gap extension penalty: 1; scoring matrix: blosum62). The amino acid identity and degree of similarity of the isolated sequences to the respective closest known public sequences was used for the selection of homologous sequences as described below.

[1056] Form 2G

[1057] Comp...

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Abstract

Polynucleotides are disclosed which are capable of enhancing a growth, yield under water-limited conditions, and / or increased tolerance to an environmental stress of a plant transformed to contain such polynucleotides. Also provided are methods of using such polynucleotides and transgenic plants and agricultural products, including seeds, containing such polynucleotides as transgenes.

Description

[0001] This application claims priority to the following U.S. provisional applications: U.S.S.N. 60 / 990,326, filed November 27, 2007; U.S.S.N. 61 / 018,711, filed January 3, 2008; U.S.S.N. 61 / 018,732, filed January 3, 2008; U.S.S.N. 61 / 043,422, filed April 9, 2008; U.S.S.N. 61 / 044,069, filed April 11, 2008; U.S.S.N. 61 / 059,984, filed June 9, 2008; and U.S.S.N. , the entire contents of each of which are incorporated herein by reference. field of invention [0002] The present invention generally relates to transgenic plants overexpressing nucleic acid sequences encoding polypeptides capable of conferring increased stress tolerance and thus increased plant growth and crop yield (under normal or abiotic stress conditions). Furthermore, the present invention relates to novel isolated nucleic acid sequences encoding compounds that confer increased tolerance to plants under abiotic stress conditions and / or increased plant growth and / or increased yield under normal or abiotic stress co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00C07K14/00
CPCC07K14/415C12N15/8273
Inventor A·舍利D·艾伦B·麦克尔西N·许P·普齐奥R·特雷泽维R·萨里亚-米兰A·麦卡斯基尔L·威尔森L·达尔尼勒R·库尔卡尼
Owner BASF PLANT SCI GMBH
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