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Artificial propagation method by hybridizing female channa maculata and male channa argus

A technology of artificial breeding and snakehead, applied in fish farming, application, climate change adaptation, etc., can solve problems that have not been reported before, and achieve the effect of reducing the impact of bad eggs, reducing damage, and reducing costs

Active Publication Date: 2010-11-24
HANGZHOU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method of carrying out large-scale crossbreeding of snakehead (female fish) and black snakehead (male fish) has not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) On April 30, 2008, 498 sexually mature and healthy individual channa females and 498 male channa were selected as parents among 561 female channa and 570 males. Sexually mature female channa The selection criteria are that the abdomen is obviously swollen and soft, the outline of the ovary is obvious, the genital pores are slightly enlarged, slightly concave, and triangular in shape, and mature eggs can be dug out.

[0025] 2) The artificial oxytocin hormone is given twice, the first injection dose is HCG (chorionic gonadotropin) 300IU+LRH-A per 1000 grams of fish body of female channa 2 (Luteinizing hormone releasing hormone analogue) 2μg, male snakehead HCG (chorionic gonadotropin) 150IU+LRH-A per kg fish body 2 (Luteinizing hormone-releasing hormone analogue) 1 μg, the second artificial oxytocin injection is given 12 hours after the first artificial oxytocin injection, and the injection dose is HCG (chorionic gonadotropin) per 1000 grams of fish body of the female...

Embodiment 2

[0030] 1) On May 15, 2009, 480 female snakeheads of sexually mature individuals were selected from 504 female snakehead snakeheads and 493 male snakehead snakeheads as female parents, and 480 male snakehead snakeheads were selected as male parents;

[0031] 2) The artificial oxytocin hormone is given twice, the first injection dose is HCG (chorionic gonadotropin) 100IU+LRH-A per 1000 grams of fish body of the female snakehead 2(Luteinizing hormone-releasing hormone analogue) 1 μg, HCG (chorionic gonadotropin) 50IU+LRH-A per 1000 grams of fish body of male snakehead 2 (luteinizing hormone-releasing hormone analogue) 0.5 μg, 10 hours later, the second injection of artificial oxytocin, the injection dose is HCG (chorionic gonadotropin) 1000IU+LRH per 1000 grams of fish body of the female snakehead. -A 2 (Luteinizing hormone-releasing hormone analogue) 5μg+DOM (diomone maleate) 5mg, male snakehead snakehead injection dosage is HCG (chorionic gonadotropin) 500IU+LRH-A per 1000g fi...

Embodiment 3

[0036] 1) On May 19, 2009, 500 female snakehead fish and 500 male snakehead fish that were sexually mature and healthy were selected as parents among 567 snakehead females and 546 snakehead males;

[0037] 2) The artificial oxytocin hormone is administered twice, the first injection dose is HCG (chorionic gonadotropin) 500IU+LRH-A per 1000 grams of fish body of the female snakehead 2 (Luteinizing hormone releasing hormone analogue) 5μg, male snakehead HCG (chorionic gonadotropin) 300IU+LRH-A per kg fish body 2 (Luteinizing hormone-releasing hormone analogue) 3 μg, the second injection of artificial oxytocin 15 hours after the first artificial induction of labor, the injection dose is HCG (chorionic gonadotropin) per 1000 grams of fish body 2000IU+LRH-A 2 (Luteinizing hormone releasing hormone analogue) 15μg+DOM (diomone maleate) 15mg, male snakehead injection dose is HCG (chorionic gonadotropin) 1000IU+LRH-A per kg fish body 2 (Luteinizing hormone releasing hormone analogue)...

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PUM

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Abstract

The invention discloses an artificial propagation method by hybridizing female channa maculata and male channa argus, which belongs to the technical field of crossbreeding of fishes. The method comprises the following steps of: picking parents; performing artificial hormone expediting for the first time; performing the artificial hormone expediting for the second time; allowing the parents to perform self-copulation and lay eggs; hatching the fertilized eggs; and hatching the fertilized eggs to break the membranes. The method has the following advantages that: simple and convenient operation and low cost are achieved by using a bubble chamber and the bubble chamber is suitable for large-scale seedling production, has a good heat preservation effect and a good shielding effect and is favorable for oestrus and spawning of the parents; through the two expediting processes, the oestrus of the male and female fishes has high synchronism, the expediting rate reaches over 96 percent and the fertilization rate reaches over 85 percent; pairing is performed after the second expediting, so that the harm caused by the male fish to the female fish is reduced and the death rate of the female fishes is ensured to be below 5 percent; the fish eggs laid by each pair of parents are individually hatched in one bubble chamber, so that the influence of bag eggs is reduced and the hatching rate is improved; and the hatching water temperature is controlled within the range from 28 to 30 DEG C, so the hatching rate is effectively increased and can reach over 85 percent.

Description

technical field [0001] The invention belongs to the technical field of fish hybrid reproduction, and in particular relates to a hybrid artificial breeding method of female snakehead and male snakehead. Background technique [0002] Crossbreeding is one of the most classic breeding methods. Obtaining hybrid F1 with advantages in growth and disease resistance through hybridization is the most widely used and effective hybrid breeding method. According to the relationship between hybrid parents, hybridization can be divided into distant hybridization and close hybridization. Distant hybridization refers to the hybridization between species, genera or even more distant biological types, while close hybridization refers to the hybridization between different strains or different geographical groups within the same species. Fish are the vertebrates most prone to distant hybridization. [0003] Since Genner obtained the world's first recorded hybrid by crossing carp and goldfish...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 冯晓宇王宇希谢楠郭水荣刘新轶姚桂桂马柏林
Owner HANGZHOU ACAD OF AGRI SCI
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