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Method for catalyzing and synthesizing 10-deacetylbaccatin (DAB) III by utilizing biocatalysts

A biocatalyst and deacetylation technology are applied in the field of catalyzing and synthesizing 10-deacetyl baccatine III by using biocatalysts, and can solve the problems of simultaneous production of 10-DABIII and the like, and achieve the effects of improving economic benefits of enterprises and reducing production costs.

Inactive Publication Date: 2010-11-24
HEFEI MEIFU BIOLOGICAL SCI
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  • Claims
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Problems solved by technology

But so far, there are only two companies producing 10-DABIII in the country, with an output of 200g / month and a maximum purity of 98.35%, and no domestic manufacturer has systematically produced 10-DABIII at the same time

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  • Method for catalyzing and synthesizing 10-deacetylbaccatin (DAB) III by utilizing biocatalysts
  • Method for catalyzing and synthesizing 10-deacetylbaccatin (DAB) III by utilizing biocatalysts

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Embodiment Construction

[0014] The present invention will be further described in detail below in conjunction with the drawings and specific embodiments.

[0015] The invention relates to a method for catalytically synthesizing 10-deacetyl baccatin III by using a biocatalyst. The waste residue after paclitaxel extraction is collected as a substrate, which mainly includes: rich in baccatin III, paclitaxel, paclitaxel C, and three tips Taxane, 7-β-xylosyl baccatine III, 7-β-xylosyl paclitaxel, 7-β-xylosyl paclitaxel C, 7-β-xylosyl cephalomannine, etc. Alkane components, because microorganisms have the ability to convert the effective components in the above waste residues into 10-deacetylbaccatine III (10-DABIII), a chemical intermediate for the synthesis of paclitaxel, so the present invention considers adding Fusarium mellifera to the waste residue And xylase is used as a biocatalyst to catalyze the waste residue to generate 10-deacetylbaccatine III (10-DABIII).

[0016] In this example, first mix the bi...

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Abstract

The invention relates to a method for catalyzing and synthesizing 10-deacetylbaccatin (DAB) III by utilizing biocatalysts. The method comprises the following step of: adding fusarium mairei and xylosidase as biocatalysts to a substrate to carry out a catalytic reaction to generate the 10-DAB, wherein the substrate is waste residues from taxol extraction. In the method, the waste residues which are obtained from the taxol extraction and contain a large quantity of taxane compounds are subjected to secondary catalysis and conversion so that the taxane compounds are converted into the 10-DAB III used as a chemical intermediate for synthesizing the taxol, thereby the requirement on raw materials of semi-synthesizing the taxol or docetaxel is greatly met and the purposes of comprehensively utilizing the waste residues from extraction, reducing the production cost and improving the economic benefits of enterprises are achieved.

Description

Technical field [0001] The invention relates to a method for catalytically synthesizing 10-deacetyl baccatin III, in particular to a method for catalytically synthesizing 10-deacetyl baccatin III by using a biocatalyst. Background technique [0002] 10-Deacetylbaccatine III (10-DABIII) is a precursor product for the synthesis of paclitaxel and docetaxel. Taxol (taxol) is the most popular broad-spectrum cancer drug substance with a unique anti-cancer mechanism after adriamycin and cisplatin. It has a significant effect on drug-resistant ovarian cancer, metastatic breast cancer, and non-small cell lung cancer. At the same time, it has been used to develop diabetes, rheumatism, nephropathy, sclerosis, Kaposi’s sarcoma and other diseases caused by AIDS. Its anti-oncogene is to promote tubulin polymerization and inhibit its depolymerization, thereby inhibiting the rapid growth of cancer cells. Split. Paclitaxel is mainly found in the bark of Taxus chinensis plants, with a content of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/02C12R1/77
Inventor 段周明吴海林
Owner HEFEI MEIFU BIOLOGICAL SCI
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