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Phase measurement method of variable exposure time imaging phase shift

A technology of exposure time and phase shift measurement, which can be used in phase influence characteristic measurement, image communication, color TV parts, etc., and can solve the problem of large phase error

Inactive Publication Date: 2013-04-03
TSINGHUA UNIV
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Problems solved by technology

Therefore, the error of the calculated phase is relatively large

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  • Phase measurement method of variable exposure time imaging phase shift
  • Phase measurement method of variable exposure time imaging phase shift
  • Phase measurement method of variable exposure time imaging phase shift

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Embodiment Construction

[0090] The phase shift method for imaging with variable exposure time provided by the present invention and its application in SPR biomolecular interaction detection will be described below with reference to the accompanying drawings.

[0091]In the experiment, it is necessary to set 5 different exposure times for 5 interference images of a phase shift period, continuously collect images and calculate the phase. However, the existing commercial CCD camera does not have the function of periodically changing the five different exposure times during continuous acquisition, so it is not applicable. The CCD camera developed by the invention can realize the above functions and meet the requirements of SPR biomolecular interaction detection. The FPGA chip in the camera acts as a timing generator to generate CCD driving timing signals, which can set different exposure times according to different detection objects, and operate synchronously with the phase shift of the electro-optic cr...

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Abstract

The invention relates to a phase measurement method of a variable exposure time imaging phase shift, belonging to the technical field of optical phase measurement. the method is characterized in that image-collecting software based on a variable exposure time imaging phase shift is established in a computer in an SPR (Surface Plasmon Resonance) imaging detection system which is modulated by a time phase, exposure time parameters including various different parameters, such as exposure time, exposure start time and exposure end time are set, and a phase shift measurement period, a frame synchronization period, an electro-optical crystal synchronization period and an exposure signal period including the exposure start time and the exposure end time are controlled by an FPGA (Field programmable Gate Array) preset in a CCD (Charge Coupled Device) camera to drive a CCD driving circuit so that images are shot one by one by a CCD chip, and the standard deviation of the phase error of p lightoutput by a polarizer is reduced by reducing the standard deviation of a light intensity measurement error in an interferogram which is generated by every phase shift. The invention has the advantagethat the error accuracy of a detected phase is improved.

Description

Technical field: [0001] The invention belongs to the technical field of optical phase measurement, and in particular relates to the application of a variable exposure time imaging method in SPR biomolecular interaction label-free real-time array phase detection. Background technique: [0002] As one of the effective methods to quantitatively obtain the phase change information of the interference signal from the interference signal, the phase shift technology has been widely used in optical detection and metrology. The basic principle is: when two wave surfaces meet and interfere, the light intensity at a point in the interferogram can be expressed as: [0003] [0004] where I i is the light intensity at a point in the interferogram of the i-th phase shift, I a and I b are the light intensities of the two beams of coherent light; is the measured phase, that is, the phase difference between the two wavefronts at the measured point in the interferogram; δ i is a cont...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/45H04N5/232
Inventor 张玮余兴龙邓焱罗昭锋
Owner TSINGHUA UNIV
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