Method for rapidly detecting activity of acetolactate synthase in rape leaves
A kind of technology of acetolactate and detection method, which is applied in the direction of measuring device, scattering characteristic measurement, color/spectral characteristic measurement, etc., can solve the problems of laborious, complicated operation and high cost, shorten the detection time, reduce environmental pollution and reduce detection cost effect
Inactive Publication Date: 2010-12-01
ZHEJIANG UNIV
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[0005] The invention provides a rapid detection method for the activity of acetolactate synthase in rapeseed leaves, which solves the problems of time-consuming, laborious, complicated operation, high cost and environmental pollution in the existing detection methods
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[0017] 248 rapeseed leaves were randomly collected. In order to make the collected rapeseed leaf samples more representative and diverse, the collected 248 leaves not only included rapeseed leaves under normal growth state (62 pieces in total), but also included sprayed rapeseed leaves. 186 pieces of rapeseed leaves with different concentrations (100, 500, 1000mg / L) of acetofen herbicide (62 pieces of rapeseed leaves were collected for each concentration gradient of 100, 500, 1000mg / L), and the spraying time was the five-leaf one-heart period. The method is foliar spraying. Propoxyfen herbicide is an ALS inhibitor, which can inhibit ALS in rapeseed leaves, and can make the ALS content in rapeseed leaves have a wider range, so that the model has better adaptability and robustness.
[0018] 248 rapeseed leaves were placed in an oven at 105°C for 30 minutes and then dried at 80°C to constant weight. The dried rapeseed leaf sample was ground into powder with a mortar, and passed ...
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The invention discloses a method for rapidly detecting the activity of acetolactate synthase in rape leaves. The method comprises the following steps of: fixing, drying, crushing rape leaves to obtain leaf powder; respectively acquiring the reflectivity of the leaf powder which corresponds to light waves with the wavelengths of 2,366 nm, 1,494 nm, 1,776 nm, 1,730 nm, 2,292 nm, 1,424 nm, 1,528 nm, 2,432 nm, 2,266 nm, 1,676 nm, 1,190 nm, 1,346 nm, 1,400 nm, 2,106 nm and 2,052 nm; and respectively substituting the reflectivity into a multiple linear regression, and calculating the activity of the acetolactate synthase in the rape leaves. In the method, a few characteristic wavelengths are subjected to multiple linear regression analysis, so the activity of the acetolactate synthetase in the rape leaves is rapidly and accurately detected, the detection time is greatly reduced, the environmental pollution is reduced, and the detection cost is reduced.
Description
technical field [0001] The invention relates to the field of rapeseed detection, in particular to a rapid detection method for the activity of acetolactate synthase in rapeseed leaves. Background technique [0002] Rapeseed is one of the main oil crops and nectar crops in my country. The planting area of rapeseed accounts for more than 40% of the total area of oil crops in the country, and its output accounts for more than 60% of the total output of edible vegetable oil in the country, ranking first in the world. The growth status of rapeseed in different periods affects the yield and quality of rapeseed. The activity of acetolactate synthase (ALS) in rapeseed leaves is a very important physiological indicator in the growth process of rapeseed. ALS can reflect the growth status of rapeseed leaves, so the growth status of rapeseed can be obtained by detecting ALS in rapeseed leaves. In recent years, due to the reduction of rural labor force, a large amount of herbicides...
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IPC IPC(8): G01N21/49G01N21/35G01N21/3563G01N21/359
Inventor 刘飞何勇孔汶汶
Owner ZHEJIANG UNIV