Method for extracting chondroitin, collagen and high-calcium powder from animal cartilage
A technology of collagen and chondroitin, applied in the field of chondroitin, collagen, and high-calcium powder extraction, can solve the problems of wasting protein resources, environmental pollution, etc., achieve good economic and social benefits, reduce acid-base consumption, and comprehensive pollution index Falling effect
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Embodiment 1
[0033] Simply wash 1 ton of fresh bovine cartilage (including costal cartilage and throat) and break it into pieces of 5-20mm, mix it with water at a ratio of 1:1, add it to the enamel reaction kettle, heat it to 100°C and keep it for 1-2 hour, ensure that the bones are cooked to a certain degree, then pass cold water into the jacket of the reaction kettle to cool down, and when the temperature is lowered to 40-50°C, add 0.01%--0.03% collagenase by weight of cartilage (dried) and add 0.1%- 0.3% papain, keep the temperature at 40-60°C, stir and hydrolyze for 8 hours, coarsely filter out the bone residue, after the bone residue is washed, dried and crushed, about 200 kg of high calcium powder is obtained. The coarsely filtered liquid is then finely filtered to obtain a clear fine filtrate, which is added with more than 90% alcohol to an alcohol mass percentage concentration of 60-70%, and the chondroitin crystals are precipitated. The liquid is a mixed solution of collagen and a...
Embodiment 2
[0035] Crush 1 kg of dried shark skulls into 5-20mm pieces, add water at a ratio of 1:4, heat to 100°C, keep for 1-2 hours, then cool down to 40-60°C, add cartilage (break dry) 0.01%-0.03% collagenase plus 0.1%-0.3% papain by weight, keep the temperature at 40-60°C, stir and hydrolyze for 8 hours, filter out the bone residue with 100 mesh, wash, dry and crush the bone residue Finally, about 300 grams of high-calcium powder were obtained; the liquid filtered out was finely filtered to obtain a clear fine filtrate, and more than 90% alcohol was added to the liquid until the concentration of alcohol mass percentage was 60-70%, the chondroitin crystals precipitated, and the liquid was collagen A mixed solution of protein and alcohol, the chondroitin crystals were washed with more than 90% alcohol, and dried to obtain 120 grams of chondroitin powder; the mixed solution was distilled to recover alcohol and then it was a collagen solution, which was concentrated to 70% concentration t...
Embodiment 3
[0037] The steps and methods are the same as in Examples 1 and 2, except that the dried pig nose bone is used as a raw material, and the pig nose bone contains about 40% chondroitin, which can be extracted with this technology, and the yield can reach 35% (the finished product has a purity of 90% as detected by the CPC method). %), proving that the present invention co-produces chondroitin while producing collagen, and its yield, product purity and appearance all reach or exceed the level of single production of chondroitin.
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