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Achromobacter xylosoxidans and application thereof for degrading o-aminobenzoic acid

A technology of anthranilic acid and achromobacter, which is applied in the direction of microorganism-based methods, bacteria, chemical instruments and methods, etc., can solve the problems of increased BOD, failure to meet emission standards, and failure to control the decolorized product anthranilic acid, etc. , to achieve the effect of high degradation efficiency and low cost

Inactive Publication Date: 2010-12-15
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Application of laccase to decolorize printing and dyeing wastewater containing indigo, BOD increased significantly after decolorization, COD remained basically unchanged, still high, and could not be discharged up to the standard
The decolorized product contains anthranilic acid. At present, the decolorization of indigo with laccase is still in the research stage, and there is no report on the method of treating the decolorized product anthranilic acid.

Method used

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  • Achromobacter xylosoxidans and application thereof for degrading o-aminobenzoic acid
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  • Achromobacter xylosoxidans and application thereof for degrading o-aminobenzoic acid

Examples

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Effect test

Embodiment 1

[0016] Example 1. Isolation, purification and identification of Achromobacter xylosoxidans N4 CGMCC No. 3632.

[0017] In May 2009, the activated sludge from the denim printing and dyeing wastewater treatment tank (collected from a denim printing and dyeing factory in Haining City, Zhejiang Province) was diluted with sterile water, and coated with solid medium (1L solid medium contains 3g of beef extract, Peptone 10g, NaCl 5g, agar 15g, and the rest are water) plate, 18 bacterial strains were isolated, and the bacterial strain was purified by streaking on the plate. The 18 isolates and purified strains were respectively inserted into the liquid medium (1L medium contains 3g of beef extract, 10g of peptone, 5g of NaCl, and the rest is water) with an inoculation loop for liquid culture. , was added to anthranilic acid aqueous solution with 5% (volume percentage) inoculum. The results of liquid chromatography showed that the N4 strain could degrade anthranilic acid quickly and ef...

Embodiment 2

[0022] Example 2. Validation test of the effect of Achromobacter xylosoxidans N4 CGMCC №.3632 on the degradation of anthranilic acid

[0023] Medium for Achromobacter xylosoxidans N4 CGMCC №.3632:

[0024] Solid medium (1L): 3g of beef extract, 10g of peptone, 5g of NaCl, 15g of agar, and the rest is water.

[0025] Liquid medium (1L): 3g of beef extract, 10g of peptone, 5g of NaCl, and the rest is water.

[0026] 1. Degradation of 100 mg / L anthranilic acid solution by Achromobacter xylosoxidans N4 CGMCC №.3632

[0027] Inoculate Achromobacter xylosoxidans (Achromobacter xylosoxidans) N4CGMCC №.3632 into the liquid medium, and cultivate it at 28°C and 180rpm for 24-48h until the OD of the culture medium is reached. 600 0.6, then in a non-sterile environment, the culture solution was inoculated into the aqueous solution of 100 mg / L anthranilic acid according to the volume percentage of 5%, and the control group was added with 5% distilled water according to the volume percent...

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Abstract

The invention discloses an achromobacter xylosoxidans bacterial strain and application thereof for degrading o-aminobenzoic acid. The bacterial strain is (Achromobacter xylosoxidans) N4, the preservation registration number thereof is CGMCC No. 3632. The (Achromobacter xylosoxidans) N4 CGMCC No. 3632 can rapidly and efficiently degrade o-aminobenzoic acid and can be applied to biological treatment on indigo printing and dyeing waste water.

Description

technical field [0001] The invention relates to a strain of Achromobacter xylosoxidans and its application in degrading anthranilic acid. Background technique [0002] Denim printing and dyeing wastewater contains a large amount of indigo, resulting in high chroma, and the biodegradability of indigo is poor, so it is difficult to directly treat the printing and dyeing wastewater containing indigo with microorganisms. The use of laccase to decolorize indigo to treat printing and dyeing wastewater has the advantages of no secondary pollution, reusability, and low cost compared with the current physical photooxidation method or the method of depositing indigo with chemical reagents, and the current denim printing and dyeing factory Wastewater contains not only indigo, but also a large amount of sulfur black dye. If laccase is used to decolorize indigo, sulfur black can be easily precipitated and recovered. Otherwise, the two dyes can be precipitated together by precipitating pr...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/025C02F101/38C02F103/30
Inventor 王雁萍董湘熔秦广雍谈重芳李宗伟常胜和李月艳
Owner ZHENGZHOU UNIV
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