Achromobacter xylosoxidans and application thereof for degrading o-aminobenzoic acid

A technology of anthranilic acid and achromobacter, which is applied in the direction of microorganism-based methods, bacteria, chemical instruments and methods, etc., can solve the problems of increased BOD, failure to meet emission standards, and failure to control the decolorized product anthranilic acid, etc. , to achieve the effect of high degradation efficiency and low cost

Inactive Publication Date: 2010-12-15
ZHENGZHOU UNIV
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  • Abstract
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Problems solved by technology

[0003] Application of laccase to decolorize printing and dyeing wastewater containing indigo, BOD increased significantly after decolorization, COD remained basically unchanged, still high, and could not be discharged up to the sta

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  • Achromobacter xylosoxidans and application thereof for degrading o-aminobenzoic acid

Examples

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Example Embodiment

[0016] Example 1. Isolation, purification and identification of Achromobacter xylosoxidans N4 CGMCC No. 3632.

[0017] In May 2009, the activated sludge in the denim printing and dyeing wastewater treatment tank (collected from a denim printing and dyeing factory in Haining, Zhejiang Province) was diluted with sterile water and coated with solid medium (1L solid medium containing 3g beef extract, Peptone 10g, NaCl 5g, agar 15g, the rest is water) plate, isolated 18 strains, using the method of streaking the plate to purify the strains. The 18 strains isolated and purified were connected to the liquid culture medium (1L medium containing beef extract 3g, peptone 10g, NaCl 5g, and the rest are water) with an inoculating loop for liquid culture. The culture temperature was 28℃ and 180rpm cultured for 24h. , Adding 5% (volume percentage) inoculum to the anthranilic acid aqueous solution, the liquid chromatography measurement results show that the N4 strain can quickly and efficiently...

Example Embodiment

[0022] Example 2. Verification test of the effect of Achromobacter xylosoxidans N4 CGMCC №.3632 on the degradation of anthranilic acid

[0023] The culture medium of Achromobacter xylosoxidans N4 CGMCC №.3632:

[0024] Solid medium (1L): beef extract 3g, peptone 10g, NaCl 5g, agar 15g, the rest is water.

[0025] Liquid medium (1L): 3g beef extract, 10g peptone, 5g NaCl, and the rest is water.

[0026] 1. Degradation of 100mg / L anthranilic acid solution by Achromobacter xylosoxidans N4 CGMCC №.3632

[0027] Inoculate Achromobacter xylosoxidans N4CGMCC №.3632 into liquid culture medium, culture it at 28℃, 180rpm for 24-48h, to the OD of culture medium 600 Then in a non-sterile environment, the culture solution was inoculated into a 100mg / L anthranilic acid aqueous solution at 5% by volume. The control group was added with 5% by volume distilled water at 28°C at 180rpm Shake or stand still, take samples and centrifuge at different times, filter the supernatant with 0.22um microporous mem...

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Abstract

The invention discloses an achromobacter xylosoxidans bacterial strain and application thereof for degrading o-aminobenzoic acid. The bacterial strain is (Achromobacter xylosoxidans) N4, the preservation registration number thereof is CGMCC No. 3632. The (Achromobacter xylosoxidans) N4 CGMCC No. 3632 can rapidly and efficiently degrade o-aminobenzoic acid and can be applied to biological treatment on indigo printing and dyeing waste water.

Description

technical field [0001] The invention relates to a strain of Achromobacter xylosoxidans and its application in degrading anthranilic acid. Background technique [0002] Denim printing and dyeing wastewater contains a large amount of indigo, resulting in high chroma, and the biodegradability of indigo is poor, so it is difficult to directly treat the printing and dyeing wastewater containing indigo with microorganisms. The use of laccase to decolorize indigo to treat printing and dyeing wastewater has the advantages of no secondary pollution, reusability, and low cost compared with the current physical photooxidation method or the method of depositing indigo with chemical reagents, and the current denim printing and dyeing factory Wastewater contains not only indigo, but also a large amount of sulfur black dye. If laccase is used to decolorize indigo, sulfur black can be easily precipitated and recovered. Otherwise, the two dyes can be precipitated together by precipitating pr...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/025C02F101/38C02F103/30
Inventor 王雁萍董湘熔秦广雍谈重芳李宗伟常胜和李月艳
Owner ZHENGZHOU UNIV
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