Application of rice OsWRKY45-1 gene in improving abiotic stress resistance of plants

A gene and plant technology, applied in plant genetic improvement, angiosperm/flowering plants, applications, etc., can solve problems such as limited good genetic resources

Inactive Publication Date: 2010-12-22
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are very limited good genetic resources available for the improvement of crop resistance to abiotic stress

Method used

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  • Application of rice OsWRKY45-1 gene in improving abiotic stress resistance of plants
  • Application of rice OsWRKY45-1 gene in improving abiotic stress resistance of plants
  • Application of rice OsWRKY45-1 gene in improving abiotic stress resistance of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Analysis of the expression pattern of OsWRKY45-1 gene under various stress responses

[0016] We selected the japonica rice variety IRAT109 (Oryza sativa L.subsp.japonica cv.) as the material for expression profile analysis. After the seeds are germinated, they are cultured to the four-leaf stage under normal growth conditions and treated with various adversities and hormones. In the drought treatment, the plants grown in sandy soil were deprived of water, and samples were taken before stress, 3 days, 4 days, and 6 days after stress. For high-salt stress, the seedlings were transferred from the hydroponic solution to the hydroponic solution containing 200mmol / L NaCl, and samples were taken before the stress, 3 hours, 6 hours, 12 hours and 24 hours after the stress. For low temperature stress, the rice seedlings were placed in an artificial climate chamber at 4°C, and samples were taken before the stress, 3 hours, 6 hours, 12 hours and 24 hours after the stre...

Embodiment 2

[0017] Example 2: Transgenic plants have enhanced tolerance to drought

[0018] In this example, a drought stress experiment was carried out on the line (2C-50229) that inhibits the expression of the OsWRKY45-1 gene and the corresponding negative line. After the above materials are germinated, they are broadcast live into small drums. The soil used in the test is a mixture of southern paddy soil and coarse sand at a volume ratio of 1:1. An equal amount of uniform sand and an equal volume of water are added to each drum, and the water will seep by itself to ensure The compactness of the soil was the same, and the experiment was repeated 3 times. When the plant grows to the 4-leaf stage, carry out water-cutting and drought stress for 3 to 5 days, then rewater, and investigate the survival rate of the plant after 3 to 7 days and take pictures. The results showed that, compared with the negative control, the line suppressing the expression of OsWRKY45-1 gene enhanced the toleranc...

Embodiment 3

[0019] Example 3: Transgenic plants have enhanced tolerance to low temperature

[0020] In this example, a low temperature stress experiment was carried out on the strain (2C-50229) that inhibits the expression of the OsWRKY45-1 gene and the corresponding negative strain. After the above materials were germinated, they were broadcast directly into small drums. The soil used in the test was common southern paddy soil, and the test was repeated 3 times. When the plants grow to the 4-leaf stage, carry out low temperature (4°C) stress for 4 to 6 days, then move the plants to normal room temperature conditions, and investigate the survival rate of the plants after recovering for 3 to 7 days and take pictures. The results showed that, compared with the negative control, the strains suppressing the expression of OsWRKY45-1 gene enhanced the tolerance to low temperature ( image 3). After recovering 7 days, the survival rate of the lines suppressing OsWRKY45-1 gene expression was 48...

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Abstract

The invention relates to the technical field of plant gene engineering, in particular to a functional verification of a rice OsWRKY45-1 gene in resisting abiotic stress. A T-DNA insertion technology is used to inhibit OsWRKY45-1 gene expression in the rice varieties. The genetic transformation rice with inhibited OsWRKY45-1 gene expression has obvious enhancement for drought and hypothermal endurance capacity, thereby proving that the OsWRKY45-1 gene is a negative control factor in resisting the abiotic stress for the rice. The invention cultivates plants with drought and hypothermy resistance by inhibiting the OsWRKY45-1 gene.

Description

technical field [0001] The invention relates to the technical field of genetic engineering. It specifically relates to the functional verification and application of rice OsWRKY45-1 gene in rice response to abiotic stress. The OsWRKY45-1 gene is a negative regulator in drought and cold responses in rice. After inhibiting the function of OsWRKY45-1 gene, the resistance ability of rice to cope with drought and low temperature was significantly improved. Background technique [0002] Crop production is affected by many environmental factors. Drought, high salinity, and low temperature are the most common abiotic stresses, which seriously affect the growth of crops, resulting in a decline in yield and quality, and are the bottleneck of agricultural development in many areas. Therefore, cultivating stress-resistant crops has always been one of the main goals of crop variety improvement. Long-term evolutionary selection has enabled plants to form a self-protection mechanism ag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29A01H5/00
Inventor 王石平陶增
Owner HUAZHONG AGRI UNIV
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