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Plant-origin drug for preventing or improving hyperuricemia

A hyperuricemia and improving agent technology, which is applied in the direction of plant raw materials, plant/algae/fungus/moss components, drug combinations, etc., can solve the problem of insufficient xanthine oxidase inhibitory effect and low safety problem, to achieve excellent xanthine oxidase inhibitory activity, high safety, effective prevention and improvement

Inactive Publication Date: 2010-12-22
KANEKA CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, many compounds with xanthine oxidase inhibitory activity have been reported, but most of them are chemically synthesized, and many of them are not safe
There are also reports of research and development of xanthine oxidase inhibitors derived from natural substances (Patent Documents 1 and 2), but these inhibitors cannot obtain a sufficiently satisfactory xanthine oxidase inhibitory effect

Method used

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  • Plant-origin drug for preventing or improving hyperuricemia
  • Plant-origin drug for preventing or improving hyperuricemia
  • Plant-origin drug for preventing or improving hyperuricemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Artemisia mugwort (whole plant), snow lotus (whole plant), sage sage (whole plant), Eurasian Huoxuedan (whole plant), peppermint (leaves), Millipede spathifolia (stem) (purchased from Shinwa Products Co., Ltd. chrysanthemum (flower), oregano (leaves and flowers) (purchased from Kaneka Sunspaisu Co., Ltd. above); violet (flower) (purchased from Xiaolingui Co., Ltd.); guava (leaf), peanut (inner bark ) (purchased from a general store above) each 1000g was immersed in 5L ethanol aqueous solution of 99.5% by volume, stirred and extracted at 45°C for 6 hours, and the residue was removed by filtration to obtain an extract. Furthermore, the extract was concentrated under reduced pressure to remove the solvent to obtain the respective extracts. In addition, Pycnogenol was a commercially available product as it was.

Embodiment 2

[0071] When evaluating the xanthine oxidase inhibitory activity of each sample, each extract prepared in Example 1 and Pycnogenol were dissolved in DMSO to make 100 mg / mL. Each DMSO solution was dissolved in 75 mM phosphate buffer (pH 7.5) to prepare a 400 µg / mL sample solution.

[0072] 50 μL of the sample solution and 50 μL of an enzyme solution in which buttermilk-derived xanthine oxidase was dissolved in phosphate buffer to a concentration of 0.2 units / mL were added to a 96-well plate, and treated at 25° C. for 15 minutes. Then, 100 μL of a 0.4 mM xanthine solution was added, and the reaction was performed at 25° C. for 15 minutes. At this time, the final concentration of each sample was 100 μg / mL. Then, 20 μL of 1N hydrochloric acid was added to stop the reaction. The absorbance at 295 nm was measured with a microplate reader. In addition, as a solvent control, 50 μL of a solution in which DMSO was dissolved in a phosphate buffer to a concentration of 0.4% was used ins...

Embodiment 3

[0081] When evaluating the xanthine oxidase inhibitory activity of each sample, occanin (from T. chinensis) and allopurinol (manufactured by Wako Pure Chemical Industries, Ltd.) were each dissolved in DMSO at 100 mg / mL. Each DMSO solution was dissolved in 75 mM phosphate buffer (pH 7.5) to prepare a 400 µM sample solution.

[0082] 50 μL of the above sample solution and 50 μL of an enzyme solution in which buttermilk-derived xanthine oxidase was dissolved in phosphate buffer to a concentration of 0.2 units / mL were added to a 96-well plate, and treated at 25° C. for 15 minutes. Then, 100 μL of a 0.4 mM xanthine solution was added, and the reaction was performed at 25° C. for 15 minutes. At this time, the final concentration of each sample was 100 μg / mL. Then, 20 μL of 1N hydrochloric acid was added to stop the reaction. The absorbance at 295 nm was measured with a microplate reader. In addition, as a solvent control, 50 μL of a solution in which DMSO was dissolved in a phosp...

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Abstract

Provided is a drug for preventing or improving hyperuricemia which comprises, as the active ingredients, an organic solvent extract or aqueous organic solvent solution extract of at least one plant material selected from the group consisting of artemisia, Saussurea involucrata, chrysanthemum, guava, cudweed (Gnaphalium affine), blue mallow, oregano, Glechoma hederaceae, mentha, Millettia reticulata and peanut, picnogenol and / or a chalcone derivative or glycoside.

Description

technical field [0001] The present invention relates to at least one plant selected from the group consisting of mugwort, snow lotus, chrysanthemum, guava, sagegrass, violet, oregano, Eurasian Huoxuedan, peppermint, milletus, peanut and pycnogenol A preventive or ameliorating agent for hyperuricemia and a xanthine oxidase inhibitor in which ingredients derived from materials or chalcone derivatives are used as active ingredients. Background technique [0002] In recent years, due to the sharp increase in intake of high-calorie, high-protein, and high-fat foods and the increase in stress, etc., the number of people suffering from hyperuricemia or gout symptoms caused by it has increased. At present, it is reported that about 20% of adult males are in the state of hyperuricemia. [0003] In general, a state in which the uric acid level in the blood is 7.0 mg / dL or more is called hyperuricemia. Usually, a simple hyperuricemia state has no subjective symptoms, but if this stat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A23L1/30A61K8/97A61K36/00A61P19/06A61P43/00C12N9/99
CPCA61K36/00A23L1/3002A23L33/105A61P19/06A61P43/00
Inventor 本田真一田中穗积岸田秀之北川雅康
Owner KANEKA CORP
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