Tissue culture and rapid propagation technology for wine bamboo

Abstract

The invention provides tissue culture and rapid propagation technology for wine bamboo. The technology comprises the steps of choosing and processing of an explant, induction culture, enrichment culture, rooting culture, transplantation and the like. In the technology, auxiliaries including active carbon, 6-benzyladenine, 6-furfurylaminopurine and naphthylacetic acid are added into an MS culture medium serving as a basis for the tissue culture in two key stages of the induction of the growth of cluster buds and the rooting of strong seedlings of the wine bamboo. The technology solves problems at the important technical links of the rapid propagation and the stable rooting of the wine bamboo and fulfills the aim of high propagation coefficient, stable technology and industrial production.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to plant tissue culture and rapid propagation technology. Background technique [0002] Wine bamboo (Oxytenanthera braunii) is a bamboo species of the Bamboo subfamily Oxytenanthera braunii, which is native to the mid-alpine region of East Africa (Liese, 1992). The culms are clustered, 6-10m high, 4-9cm in diameter, the underground stems are biaxial, without whip; the culm is cylindrical, with no longitudinal groove on the side of the branch; the internode length is 25-45cm, and each node of the culm has 5-9 branches. One or more, with secondary branches; 1-2 main branches on the same node of the culm that are obviously different from other branches. During the formation of new bamboo (high growth of bamboo shoots), new bamboo stalks secrete wound fluid with high alcohol content in the first few days after cutting the shoots. As an important living and economic bamboo species, This na...

AI Technical Summary

Problems solved by technology

[0003] Although farmers in the place of origin work wine bamboo as a kind of fixed income, the bamboo species are distributed in the mountainous areas of eastern Africa, where the distribution is primitive and remote, the transportation is inconvenient, and the distribution area is small, so the production and operation are almost in the In the original state, therefore, the research, development and utilization of wine bamboo are still in the initial stage in the world

At the same time, due to years of indiscriminate digging by people and some restrictions on their reproductive mechanism and micro-habitat requirements, the distribution area and quantity of wine bamboo suitable for growth are not large, and it is difficult to meet the needs of the industrial field.