Method for stimulating gene exchange among sweet potato germplasm sources

A gene exchange and sweet potato technology, applied in the field of seed improvement, can solve problems such as incompatibility, incompatibility seed setting rate, self-incompatibility, etc., and achieve the effect of not being limited by seasons, enriching innovative methods, and convenient operation

Inactive Publication Date: 2011-01-05
JIANGSU XUZHOU SWEET POTATO RES CENT
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Problems solved by technology

At present, the innovation of sweet potato germplasm mainly adopts sexual hybridization, genetic transformation, somatic cell hybridization, mutagenesis, etc.; due to the self-incompatibility of sweet potato, some interspecific hybridization incompatibility, and even hybridization incompatibility between varieties in the same group The genetic improvement of sweet potato has been delayed to some extent due to low seed setting rate and genotype dependence.

Method used

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  • Method for stimulating gene exchange among sweet potato germplasm sources
  • Method for stimulating gene exchange among sweet potato germplasm sources
  • Method for stimulating gene exchange among sweet potato germplasm sources

Examples

Experimental program
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Effect test

Embodiment 1

[0014] In this experiment, firstly, Jishu 18 and Zhengshu 20 needed for grafting were regenerated by culturing the shoot apical meristem. The shoot tip meristem medium condition is 1LMS basic medium with NAA0.1mg / L and 6-BA2mg / L, sucrose 30g / L, agar 9g / L, and the pH value before sterilization is 5.8-6.0. The culture condition is light 2000Lux, temperature 25±2℃.

[0015] The shoot apex meristems of Jishu 18 and Zhengshu 20 were used to cultivate regenerated plants, the thickness of which was relatively similar or the rootstock was thicker than the scion. In the ultra-clean workbench (under sterile conditions), Zhengshu 20 was used as the rootstock, and Jishu 18 was used as the scion; 2-3 nodes of the scion plant were cut, and the incision was wedge-shaped; all leaves and germinated axillary buds were removed from the rootstock material, and the interface Split from the middle at a depth of about 0.5cm, then insert the scion, wrap the grafted wound with sterile Parafilm, and i...

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Abstract

The invention discloses a method for stimulating the gene exchange among sweet potato germplasm sources, belonging to the fields of obtaining donor and recipient plants through the tissue culture of sweet potatoes. The method comprises the following steps of: asexually engrafting donors and recipient plants under the in-vitro and sterile conditions, stimulating introgression among sweet potato breeds, creating sweet potato germplasm for the gene exchange among the sweet potato breeds and different incompatibility group breeds; and further creating and enriching the sweet potato germplasm sources. The invention has the advantages of breaking the crossing barrier among breeds and different incompatibility group breeds, providing a new technical method for creating the sweet potato germplasm and stimulating the genetic improvement of sweet potatoes. The method is convenient and rapid to operate without season restrictions.

Description

technical field [0001] The invention relates to a seed improvement technology, in particular to a method for promoting gene exchange among germplasm resources of sweet potato. Background technique [0002] As an important feed, processing, grain and bioenergy crop, sweet potato has the characteristics of high yield, stable yield, wide adaptability and strong disease resistance. It is still one of the main crops widely planted in my country. Since my country is not the origin center of sweet potato and sweet potato resources are relatively scarce, the breeding of fine new varieties requires abundant excellent germplasm resources, and the innovation and utilization of germplasm is the basis of breeding. At present, the innovation of sweet potato germplasm mainly adopts sexual hybridization, genetic transformation, somatic cell hybridization, mutagenesis, etc.; due to the self-incompatibility of sweet potato, some interspecific hybridization incompatibility, and even hybridizat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/06
Inventor 周志林唐君曹清河赵冬兰史新敏
Owner JIANGSU XUZHOU SWEET POTATO RES CENT
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