Method for stimulating gene exchange among sweet potato germplasm sources
A gene exchange and sweet potato technology, applied in the field of seed improvement, can solve problems such as incompatibility, incompatibility seed setting rate, self-incompatibility, etc., and achieve the effect of not being limited by seasons, enriching innovative methods, and convenient operation
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[0014] In this experiment, firstly, Jishu 18 and Zhengshu 20 needed for grafting were regenerated by culturing the shoot apical meristem. The shoot tip meristem medium condition is 1LMS basic medium with NAA0.1mg / L and 6-BA2mg / L, sucrose 30g / L, agar 9g / L, and the pH value before sterilization is 5.8-6.0. The culture condition is light 2000Lux, temperature 25±2℃.
[0015] The shoot apex meristems of Jishu 18 and Zhengshu 20 were used to cultivate regenerated plants, the thickness of which was relatively similar or the rootstock was thicker than the scion. In the ultra-clean workbench (under sterile conditions), Zhengshu 20 was used as the rootstock, and Jishu 18 was used as the scion; 2-3 nodes of the scion plant were cut, and the incision was wedge-shaped; all leaves and germinated axillary buds were removed from the rootstock material, and the interface Split from the middle at a depth of about 0.5cm, then insert the scion, wrap the grafted wound with sterile Parafilm, and i...
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